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NOXA Antibody (PA5-100646) in ICC/IF
Immunocytochemistry analysis of NOXA in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NOXA polyclonal antibody (Product # PA5-100646) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100646
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PMAIP1(Accession Q13794), corresponding to amino acid residues M1-G33.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850151
Product Specific Information
Antibody detects endogenous levels of total NOXA.
Target Information
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified, which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9. Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53. Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adult T cell leukemia-derived PMA-responsive; Immediate-early-response protein APR; Phorbol-12-myristate-13-acetate-induced protein 1; PMA-ind; PMA-induced protein 1; Protein Noxa
Gene Aliases: APR; NOXA; PMAIP1
UniProt ID: (Human) Q13794
Entrez Gene ID: (Human) 5366
release of cytochrome c from mitochondria
protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
response to UV
response to X-ray
proteasomal protein catabolic process
positive regulation of glucose metabolic process
negative regulation of mitochondrial membrane potential
positive regulation of protein oligomerization
cellular response to glucose starvation
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
regulation of apoptotic process
T cell homeostasis
positive regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
response to dsRNA
positive regulation of DNA damage response, signal transduction by p53 class mediator
positive regulation of neuron apoptotic process
regulation of mitochondrial membrane permeability
negative regulation of fibroblast proliferation
defense response to virus
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hypoxia
intrinsic apoptotic signaling pathway by p53 class mediator
reactive oxygen species metabolic process
positive regulation of release of cytochrome c from mitochondria
intrinsic apoptotic signaling pathway
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
positive regulation of extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway
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