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Product Details
MA5-32679
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JA30-03
Immunogen
Synthetic peptide within Human Noxa aa 5-54
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809956
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified, which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9. Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53. Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adult T cell leukemia-derived PMA-responsive; Immediate-early-response protein APR; Phorbol-12-myristate-13-acetate-induced protein 1; PMA-ind; PMA-induced protein 1; Protein Noxa
Gene Aliases: APR; NOXA; PMAIP1
UniProt ID: (Human) Q13794
Entrez Gene ID: (Human) 5366
release of cytochrome c from mitochondria
protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
response to UV
response to X-ray
proteasomal protein catabolic process
positive regulation of glucose metabolic process
negative regulation of mitochondrial membrane potential
positive regulation of protein oligomerization
cellular response to glucose starvation
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
regulation of apoptotic process
T cell homeostasis
positive regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
response to dsRNA
positive regulation of DNA damage response, signal transduction by p53 class mediator
positive regulation of neuron apoptotic process
regulation of mitochondrial membrane permeability
negative regulation of fibroblast proliferation
defense response to virus
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hypoxia
intrinsic apoptotic signaling pathway by p53 class mediator
reactive oxygen species metabolic process
positive regulation of release of cytochrome c from mitochondria
intrinsic apoptotic signaling pathway
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
positive regulation of extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway
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