Proteintech

NPHS2 Polyclonal Antibody

NPHS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NPHS2 Antibody (20384-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 20384-1-AP (NPHS2 antibody) at dilution of 1:500 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NPHS2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

20384-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:250-1:1,000

Immunocytochemistry (ICC/IF)

1:20-1:200

View 18 publications 18 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NPHS2 Fusion Protein Ag14213 (124-315 aa encoded by BC029141)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: CVKVVQEYE RVIIFRLGHL LPGRAKGPGL FFFLPCLDTY HKVDLRLQTL EIPFHEVALD SVTCIWGIKV ERIEIKDVRL PAGLQHSLAV EAEAQRQAKV RMIAAEAEKA ASESLRMAAE ILSGTPAAVQ LRYLHTLQSL STEKPSTVVL PLPFDLLNCL SSPSNRTQGS LPFPSPSKPV EPLNPKKKDS PML (124-315 aa encoded by BC029141)

Target Information

The pathological conditions of SRN1 correlate well with mutations at the NPHS2 gene, where expression of a protein known as Podocin occurs. Abnormal or inefficient signaling through Podocin protein-dependent networks contributes to the development of podocyte dysfunction and proteinuria. The human NPHS2 gene maps to chromosome 1q25-q31 and encodes a 383 amino acid protein. Podocin is an integral membrane protein that appears to fold into a hairpin-like structure with intracellular amino- and carboxy-termini. Transmembrane and cytoplasmic portions of Podocin share homology to the corresponding regions of the stomatin family proteins. Expression of high-order oligomers of Podocin in glomerular podocytes may reflect a scaffolding function that influences proper function of the glomerular filtration barrier, which is necessary for renal stability.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Minnelide Markedly Reduces Proteinuria in Mice with Adriamycin Nephropathy by Protecting Against Podocyte Injury.

Applied biochemistry and biotechnology

Ji B,Liu J,Ma Y,Yin Y,Xu H,Shen Q,Yu J

Fri Dec 01 00:00:00 EST 2023

mTOR/ULK1 signaling axis mediated ultrashort wave regulation of autophagy to alleviate diabetic kidney disease.

Advances in clinical and experimental medicine : official organ Wroclaw Medical University

Yan Y,Yu S,Fan Y

Wed Nov 01 00:00:00 EDT 2023

Minnelide combined with Angptl3 knockout completely protects mice with adriamycin nephropathy via suppression of TGF-β1-Smad2 and p53 pathways.

International immunopharmacology

Ji B,Liu J,Ma Y,Yin Y,Xu H,Shen Q,Yu J

Wed Feb 01 00:00:00 EST 2023

Minnelide combined with Angptl3 knockout completely protects mice with adriamycin nephropathy via suppression of TGF-β1-Smad2 and p53 pathways.

International immunopharmacology

Ji B,Liu J,Ma Y,Yin Y,Xu H,Shen Q,Yu J

Wed Feb 01 00:00:00 EST 2023

Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Song D,Zhang A,Hu X,Zeng M,Zhou H

Tue Jul 25 00:00:00 EDT 2023

Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Song D,Zhang A,Hu X,Zeng M,Zhou H

Tue Jul 25 00:00:00 EDT 2023

Qian Yang Yu Yin granule improves hypertensive renal damage: A potential role for TRPC6-CaMKKβ-AMPK-mTOR-mediated autophagy.

Journal of ethnopharmacology

Ma S,Xu J,Zheng Y,Li Y,Wang Y,Li H,Fang Z,Li J

Fri Feb 10 00:00:00 EST 2023

Placental Mesenchymal Stem Cells Alleviate Podocyte Injury in Diabetic Kidney Disease by Modulating Mitophagy via the SIRT1-PGC-1alpha-TFAM Pathway.

International journal of molecular sciences

Han X,Wang J,Li R,Huang M,Yue G,Guan L,Deng Y,Cai W,Xu J

Tue Feb 28 00:00:00 EST 2023

Placental Mesenchymal Stem Cells Alleviate Podocyte Injury in Diabetic Kidney Disease by Modulating Mitophagy via the SIRT1-PGC-1alpha-TFAM Pathway.

International journal of molecular sciences

Han X,Wang J,Li R,Huang M,Yue G,Guan L,Deng Y,Cai W,Xu J

Tue Feb 28 00:00:00 EST 2023

Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Ai Z,Wang M,Zhou Y,Yuan D,Jian Q,Wu S,Liu B,Yang Y

Mon May 01 00:00:00 EDT 2023

Mapping of the podocin proximity-dependent proteome reveals novel components of the kidney podocyte foot process.

Frontiers in cell and developmental biology

Gerlach GF,Imseis ZH,Cooper SL,Santos AN,O'Brien LL

Wed Nov 08 00:00:00 EST 2023

Mapping of the podocin proximity-dependent proteome reveals novel components of the kidney podocyte foot process.

Frontiers in cell and developmental biology

Gerlach GF,Imseis ZH,Cooper SL,Santos AN,O'Brien LL

Wed Nov 08 00:00:00 EST 2023

Podocyte-specific deletion of ubiquitin carboxyl-terminal hydrolase L1 causes podocyte injury by inducing endoplasmic reticulum stress.

Cellular and molecular life sciences : CMLS

Hu Y,Qi C,Shi J,Tan W,Zhao Z,Xu Y,Wu H,Zhang Z

Thu Mar 23 00:00:00 EDT 2023

Podocyte-specific deletion of ubiquitin carboxyl-terminal hydrolase L1 causes podocyte injury by inducing endoplasmic reticulum stress.

Cellular and molecular life sciences : CMLS

Hu Y,Qi C,Shi J,Tan W,Zhao Z,Xu Y,Wu H,Zhang Z

Thu Mar 23 00:00:00 EDT 2023

Peroxisome Proliferator-Activated Receptor Gene Knockout Promotes Podocyte Injury in Diabetic Mice.

BioMed research international

Yan R,Zhang Y,Yang Y,Liu L,Liu L,Guo Z,Yu H,Wang Y,Guo B

Tue Jul 12 00:00:00 EDT 2022

Peroxisome Proliferator-Activated Receptor Gene Knockout Promotes Podocyte Injury in Diabetic Mice.

BioMed research international

Yan R,Zhang Y,Yang Y,Liu L,Liu L,Guo Z,Yu H,Wang Y,Guo B

Tue Jul 12 00:00:00 EDT 2022

Yi-Shen-Hua-Shi Granule Alleviates Adriamycin-Induced Glomerular Fibrosis by Suppressing the BMP2/Smad Signaling Pathway.

Frontiers in pharmacology

Tan Z,Si Y,Yu Y,Ding J,Huang L,Xu Y,Zhang H,Lu Y,Wang C,Yu B,Yuan L

Sat Jul 16 00:00:00 EDT 2022

Canagliflozin reverses Th1/Th2 imbalance and promotes podocyte autophagy in rats with membranous nephropathy.

Frontiers in immunology

Lv X,Wang J,Zhang L,Shao X,Lin Y,Liu H,Ma G,Li J,Zhou S,Yu P

Tue Dec 20 00:00:00 EST 2022

Canagliflozin reverses Th1/Th2 imbalance and promotes podocyte autophagy in rats with membranous nephropathy.

Frontiers in immunology

Lv X,Wang J,Zhang L,Shao X,Lin Y,Liu H,Ma G,Li J,Zhou S,Yu P

Tue Dec 20 00:00:00 EST 2022

Effects of Shenkang Pills on Early-Stage Diabetic Nephropathy in db/db Mice via Inhibiting AURKB/RacGAP1/RhoA Signaling Pathway.

Frontiers in pharmacology

Wang F,Fan J,Pei T,He Z,Zhang J,Ju L,Han Z,Wang M,Xiao W

Tue Mar 01 00:00:00 EST 2022

ManNAc protects against podocyte pyroptosis via inhibiting mitochondrial damage and ROS/NLRP3 signaling pathway in diabetic kidney injury model.

International immunopharmacology

Gao Y,Ma Y,Xie D,Jiang H

Wed Jun 01 00:00:00 EDT 2022

Experimental study on renoprotective effect of intermedin on diabetic nephropathy.

Molecular and cellular endocrinology

Wang Y,Tian J,Mi Y,Ren X,Lian S,Kang J,Wang J,Zang H,Wu Z,Yang J,Qiao X,Zhou X,Wang G,Zhou Y,Li R

Sat May 15 00:00:00 EDT 2021

Carnosine alleviates podocyte injury in diabetic nephropathy by targeting caspase-1-mediated pyroptosis.

International immunopharmacology

Zhu W,Li YY,Zeng HX,Liu XQ,Sun YT,Jiang L,Xia LL,Wu YG

Wed Dec 01 00:00:00 EST 2021

Carnosine alleviates podocyte injury in diabetic nephropathy by targeting caspase-1-mediated pyroptosis.

International immunopharmacology

Zhu W,Li YY,Zeng HX,Liu XQ,Sun YT,Jiang L,Xia LL,Wu YG

Wed Dec 01 00:00:00 EST 2021

An integrated transcriptomics and network pharmacology approach to exploring the mechanism of adriamycin-induced kidney injury.

Chemico-biological interactions

He S,Li A,Zhang W,Zhang L,Liu Y,Li K,Qin X

Wed Jul 01 00:00:00 EDT 2020

Prolonged podocyte depletion in larval zebrafish resembles mammalian focal and segmental glomerulosclerosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hansen KUI,Siegerist F,Daniel S,Schindler M,Iervolino A,Blumenthal A,Daniel C,Amann K,Zhou W,Endlich K,Endlich N

Tue Dec 01 00:00:00 EST 2020

Adriamycin does not damage podocytes of zebrafish larvae.

PloS one

Schindler M,Blumenthal A,Moeller MJ,Endlich K,Endlich N

Mon Jan 04 00:00:00 EST 2021

Paecilomyces cicadae-fermented Radix astragali activates podocyte autophagy by attenuating PI3K/AKT/mTOR pathways to protect against diabetic nephropathy in mice.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Yang F,Qu Q,Zhao C,Liu X,Yang P,Li Z,Han L,Shi X

Tue Sep 01 00:00:00 EDT 2020

Paecilomyces cicadae-fermented Radix astragali activates podocyte autophagy by attenuating PI3K/AKT/mTOR pathways to protect against diabetic nephropathy in mice.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Yang F,Qu Q,Zhao C,Liu X,Yang P,Li Z,Han L,Shi X

Tue Sep 01 00:00:00 EDT 2020

A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros.

Methods in molecular biology (Clifton, N.J.)

Kolatsi-Joannou M,Osborn D

Fri Dec 18 00:00:00 EST 2020

Exploration the active compounds of Astragali Radix in treatment of adriamycin nephropathy by network pharmacology combined with transcriptomic approach.

Journal of ethnopharmacology

Li AP,He SS,Zhang WN,Zhang LC,Liu YT,Li K,Qin XM

Mon Aug 10 00:00:00 EDT 2020

Plakoglobin is involved in cytoskeletal rearrangement of podocytes under the regulation of UCH-L1.

Biochemical and biophysical research communications

Fang Y,Li F,Qi C,Mao X,Xu Y,Zhao Z,Wu H,Zhang Z

Thu Aug 13 00:00:00 EDT 2020

Expression and distribution of PIEZO1 in the mouse urinary tract.

American journal of physiology. Renal physiology

Dalghi MG,Clayton DR,Ruiz WG,Al-Bataineh MM,Satlin LM,Kleyman TR,Ricke WA,Carattino MD,Apodaca G

Thu Aug 01 00:00:00 EDT 2019

MiR-320a induces diabetic nephropathy via inhibiting MafB.

Aging

He M,Wang J,Yin Z,Zhao Y,Hou H,Fan J,Li H,Wen Z,Tang J,Wang Y,Wang DW,Chen C

Fri May 17 00:00:00 EDT 2019

Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/β-Catenin Pathway in an Acute Kidney Injury Mouse Model.

Inflammation

Senouthai S,Wang J,Fu D,You Y

Thu Aug 01 00:00:00 EDT 2019

Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/β-Catenin Pathway in an Acute Kidney Injury Mouse Model.

Inflammation

Senouthai S,Wang J,Fu D,You Y

Thu Aug 01 00:00:00 EDT 2019

Critical roles of PI3K/Akt/NF‑κB survival axis in angiotensin II‑induced podocyte injury.

Molecular medicine reports

Wang J,Fu D,Senouthai S,You Y

Sun Dec 01 00:00:00 EST 2019

Constructing an Isogenic 3D Human Nephrogenic Progenitor Cell Model Composed of Endothelial, Mesenchymal, and SIX2-Positive Renal Progenitor Cells.

Stem cells international

Nguyen L,Spitzhorn LS,Adjaye J

Wed Sep 30 00:00:00 EDT 2020

Inhibiting 4E-BP1 re-activation represses podocyte cell cycle re-entry and apoptosis induced by adriamycin.

Cell death & disease

Li F,Mao X,Zhuang Q,Zhao Z,Zhang Z,Wu H

Mon Mar 11 00:00:00 EDT 2019

Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5.

Kidney & blood pressure research

Yang X,Ding Y,Yang M,Yu L,Hu Y,Deng Y

Mon Oct 29 00:00:00 EDT 2018

Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5.

Kidney & blood pressure research

Yang X,Ding Y,Yang M,Yu L,Hu Y,Deng Y

Mon Oct 29 00:00:00 EDT 2018

Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5.

Kidney & blood pressure research

Yang X,Ding Y,Yang M,Yu L,Hu Y,Deng Y

Mon Oct 29 00:00:00 EDT 2018

Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5.

Kidney & blood pressure research

Yang X,Ding Y,Yang M,Yu L,Hu Y,Deng Y

Mon Oct 29 00:00:00 EDT 2018

Weighted Gene Correlation Network Analysis (WGCNA) Detected Loss of MAGI2 Promotes Chronic Kidney Disease (CKD) by Podocyte Damage.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Zuo Z,Shen JX,Pan Y,Pu J,Li YG,Shao XH,Wang WP

Tue Dec 11 00:00:00 EST 2018

Quercetin ameliorates podocyte injury via inhibition of oxidative stress and the TGF-β1/Smad pathway in DN rats.

RSC advances

Gao F,He X,Liang S,Liu S,Liu H,He Q,Chen L,Jiang H,Zhang Y

Mon Oct 15 00:00:00 EDT 2018

Quercetin ameliorates podocyte injury via inhibition of oxidative stress and the TGF-β1/Smad pathway in DN rats.

RSC advances

Gao F,He X,Liang S,Liu S,Liu H,He Q,Chen L,Jiang H,Zhang Y

Mon Oct 15 00:00:00 EDT 2018

Response of Renal Podocytes to Excessive Hydrostatic Pressure: a Pathophysiologic Cascade in a Malignant Hypertension Model.

Kidney & blood pressure research

Abu Hamad R,Berman S,Hachmo Y,Stark M,Hasan F,Doenyas-Barak K,Efrati S

Thu Aug 30 00:00:00 EDT 2018

Response of Renal Podocytes to Excessive Hydrostatic Pressure: a Pathophysiologic Cascade in a Malignant Hypertension Model.

Kidney & blood pressure research

Abu Hamad R,Berman S,Hachmo Y,Stark M,Hasan F,Doenyas-Barak K,Efrati S

Thu Aug 30 00:00:00 EDT 2018

Acute podocyte injury is not a stimulus for podocytes to migrate along the glomerular basement membrane in zebrafish larvae.

Scientific reports

Siegerist F,Blumenthal A,Zhou W,Endlich K,Endlich N

Thu Mar 02 00:00:00 EST 2017

Acute podocyte injury is not a stimulus for podocytes to migrate along the glomerular basement membrane in zebrafish larvae.

Scientific reports

Siegerist F,Blumenthal A,Zhou W,Endlich K,Endlich N

Thu Mar 02 00:00:00 EST 2017

Glomeruli or interstitium targeted by inter-renal injections supplemented by electroporation: Still a useful tool in renal research.

The journal of gene medicine

Mao X,Li F,Yang N,Qi C,Zhang SQ,Zhang Z,Wu H

Tue Nov 01 00:00:00 EDT 2016

Effects of asiaticoside on levels of podocyte cytoskeletal proteins and renal slit diaphragm proteins in adriamycin-induced rat nephropathy.

Life sciences

Wang Z,Liu J,Sun W

Tue Sep 03 00:00:00 EDT 2013

Effects of asiaticoside on levels of podocyte cytoskeletal proteins and renal slit diaphragm proteins in adriamycin-induced rat nephropathy.

Life sciences

Wang Z,Liu J,Sun W

Tue Sep 03 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: nephrosis 2 homolog, podocin; nephrosis 2 idiopathic steroid-resistant (podocin); nephrosis 2, idiopathic, steroid-resistant (podocin); Podocin

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Gene Aliases: AI790225; Nphs2; PDCN; podocin; SRN1

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UniProt ID: (Mouse) Q91X05, (Rat) Q8K4G9

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Entrez Gene ID: (Mouse) 170484, (Rat) 170672

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Function(s)

protein binding molecular_function cytoskeletal protein

Process(es)

excretion actin cytoskeleton reorganization biological_process metanephric glomerular visceral epithelial cell development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NPHS2 Polyclonal Antibody

NPHS2 Antibody (20384-1-AP) in IHC (P)

Product Details

20384-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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