Invitrogen

NPM1 (mutant) Polyclonal Antibody

8 Published Figures

14 References

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (8)

NPM1 (mutant) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

NPM1 (mutant) Antibody (PA1-46356) in ICC/IF

Immunocytochemistry analysis of NPM1 (mutant) in HL-60 cells (negative control). Samples were incubated in NPM1 (mutant) polyclonal antibody (Product # PA1-46356). Nucleophosmin localization by immunofluorescence.

NPM1 (mutant) Antibody in Western Blot (WB)

NPM1 (mutant) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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Product Details

PA1-46356

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:50-1:100

View 2 publications 2 publications

Flow Cytometry (Flow)

1:100

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a C-terminal region (within residues 250-294) of human mutant nucleophosmin.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2267471

Product Specific Information

Suggested positive control: antigen standard for NPM1 (transient overexpression lysate), OCI-AML3 whole cell lysate.

HL-60 lysate can be used as a negative control for wildtype protein.

Target Information

NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cytoplasmic Expression of TP53INP2 Modulated by Demethylase FTO and Mutant NPM1 Promotes Autophagy in Leukemia Cells.

International journal of molecular sciences

Huang J,Sun M,Tao Y,Ren J,Peng M,Jing Y,Xiao Q,Yang J,Lin C,Lei L,Yang Z,Zhang L

PA1-46356 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to indicate that TP53INP2 plays an oncogenic role in maintaining the high autophagy activity of NPM1-mutated AML and provides further insight into autophagy-targeted therapy of this leukemia subtype.

Fri Jan 13 00:00:00 EST 2023

The clinicopathologic significance of NPM1 mutation and ability to detect mutated NPM1 by immunohistochemistry in non-AML myeloid neoplasms.

Genes, chromosomes & cancer

Kaseb H,Visconte V,Socha DS,Crane GM,Durkin L,Cook JR,Maciejewski JP,Hsi ED,Rogers HJ

PA1-46356 was used in Immunohistochemistry to demonstrated that IHC for NPM1 can be a useful supplementary tool to predict NPM1 mutation in some non-AML MN; however, genetic testing cannot be replaced by IHC assessment.

Sun Oct 01 00:00:00 EDT 2023

Mutant NPM1-Regulated FTO-Mediated m⁶A Demethylation Promotes Leukemic Cell Survival via PDGFRB/ERK Signaling Axis.

Frontiers in oncology

Xiao Q,Lei L,Ren J,Peng M,Jing Y,Jiang X,Huang J,Tao Y,Lin C,Yang J,Sun M,Tang L,Wei X,Yang Z,Zhang L

Published figure using NPM1 (mutant) polyclonal antibody (Product # PA1-46356) in Western Blot

Sat Feb 26 00:00:00 EST 2022

EAPB0503, an Imidazoquinoxaline Derivative Modulates SENP3/ARF Mediated SUMOylation, and Induces NPM1c Degradation in NPM1 Mutant AML.

International journal of molecular sciences

Skayneh H,Jishi B,Hleihel R,Hamie M,El Hajj R,Deleuze-Masquefa C,Bonnet PA,El Sabban M,El Hajj H

PA1-46356 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to provide a strong rationale for testing therapies modulating NPM1c post-translational modifications in the management of NPM1c AML.

Tue Mar 22 00:00:00 EDT 2022

EAPB0503, an Imidazoquinoxaline Derivative Modulates SENP3/ARF Mediated SUMOylation, and Induces NPM1c Degradation in NPM1 Mutant AML.

International journal of molecular sciences

Skayneh H,Jishi B,Hleihel R,Hamie M,El Hajj R,Deleuze-Masquefa C,Bonnet PA,El Sabban M,El Hajj H

Tue Mar 22 00:00:00 EDT 2022

Role of immunohistochemistry and flow cytometry in minimal residual disease detection in NPM1-mutated AML.

International journal of laboratory hematology

Aggarwal N,Alston ELJ,Pinkus GS,Weinberg OK

PA1-46356 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to study the role of immunohistochemistry and flow cytometry in minimal residual disease detection in NPM1-mutated AML.

Fri Apr 01 00:00:00 EDT 2022

Biological Effects of BET Inhibition by OTX015 (MK-8628) and JQ1 in NPM1-Mutated (NPM1c) Acute Myeloid Leukemia (AML).

Biomedicines

Djamai H,Berrou J,Dupont M,Coudé MM,Delord M,Clappier E,Marceau-Renaut A,Kaci A,Raffoux E,Itzykson R,Berthier C,Wu HC,Hleihel R,Bazarbachi A,de Thé H,Baruchel A,Gardin C,Dombret H,Braun T

Published figure using NPM1 (mutant) polyclonal antibody (Product # PA1-46356) in Western Blot

Wed Nov 17 00:00:00 EST 2021

Biological Effects of BET Inhibition by OTX015 (MK-8628) and JQ1 in NPM1-Mutated (NPM1c) Acute Myeloid Leukemia (AML).

Biomedicines

Djamai H,Berrou J,Dupont M,Coudé MM,Delord M,Clappier E,Marceau-Renaut A,Kaci A,Raffoux E,Itzykson R,Berthier C,Wu HC,Hleihel R,Bazarbachi A,de Thé H,Baruchel A,Gardin C,Dombret H,Braun T

Published figure using NPM1 (mutant) polyclonal antibody (Product # PA1-46356) in Western Blot

Wed Nov 17 00:00:00 EST 2021

Mutant NPM1-regulated lncRNA HOTAIRM1 promotes leukemia cell autophagy and proliferation by targeting EGR1 and ULK3.

Journal of experimental & clinical cancer research : CR

Jing Y,Jiang X,Lei L,Peng M,Ren J,Xiao Q,Tao Y,Tao Y,Huang J,Wang L,Tang Y,Yang Z,Yang Z,Zhang L

PA1-46356 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to indicate that HOTAIRM1 may be a promising therapeutic target for this distinct leukemia subtype.

Wed Oct 06 00:00:00 EDT 2021

Mutant NPM1-regulated lncRNA HOTAIRM1 promotes leukemia cell autophagy and proliferation by targeting EGR1 and ULK3.

Journal of experimental & clinical cancer research : CR

Jing Y,Jiang X,Lei L,Peng M,Ren J,Xiao Q,Tao Y,Tao Y,Huang J,Wang L,Tang Y,Yang Z,Yang Z,Zhang L

Wed Oct 06 00:00:00 EDT 2021

Abstracts from USCAP 2021: Hematopathology (664-756).

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

No authors listed

PA1-46356 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to study issues in hematopathology.

Mon Mar 01 00:00:00 EST 2021

Comparison of Multiple Clinical Testing Modalities for Assessment of NPM1-Mutant AML.

Frontiers in oncology

Lopez A,Patel S,Geyer JT,Racchumi J,Chadburn A,Simonson P,Ouseph MM,Inghirami G,Mencia-Trinchant N,Guzman ML,Gomez-Arteaga A,Lee S,Desai P,Ritchie EK,Roboz GJ,Tam W,Kluk MJ

PA1-46356 was used in Immunohistochemistry to describe and compare the performance of RT-PCR compared to traditional NGS, immunohistochemical analysis, and flow cytometry for the detection of mutant NPM1 in clinical practice.

Tue Apr 26 00:00:00 EDT 2022

Multiparametric in situ imaging of NPM1-mutated acute myeloid leukemia reveals prognostically-relevant features of the marrow microenvironment.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Patel SS,Lipschitz M,Pinkus GS,Weirather JL,Pozdnyakova O,Mason EF,Inghirami G,Hasserjian RP,Rodig SJ,Weinberg OK

PA1-46356 was used in Immunohistochemistry to show that the quantitation of specific bone marrow populations in NPM1-AML by MIF in the bone marrow biopsy and by orthogonal methodologies (FC and NGS) performed on bone marrow aspirate material, is not absolutely concordant.

Wed Jul 01 00:00:00 EDT 2020

miRNA-mRNA Profiling Reveals Prognostic Impact of SMC1A Expression in Acute Myeloid Leukemia.

Oncology research

Gadewal N,Kumar R,Aher S,Gardane A,Gaur T,Varma AK,Khattry N,Hasan SK

PA1-46356 was used in Western Blotting to suggest that dynamic miR-215-SMC1A regulation is potentially modulated by NPM1 mutation, which might serve as an explanation for the better outcome in NPM1 mutated AML.

Fri May 29 00:00:00 EDT 2020

miRNA-mRNA Profiling Reveals Prognostic Impact of SMC1A Expression in Acute Myeloid Leukemia.

Oncology research

Gadewal N,Kumar R,Aher S,Gardane A,Gaur T,Varma AK,Khattry N,Hasan SK

Fri May 29 00:00:00 EDT 2020

Up-regulation of EMT-related gene VCAN by NPM1 mutant-driven TGF-β/cPML signalling promotes leukemia cell invasion.

Journal of Cancer

Yang L,Wang L,Yang Z,Jin H,Zou Q,Zhan Q,Tang Y,Tao Y,Lei L,Jing Y,Jiang X,Zhang L

Published figure using NPM1 (mutant) polyclonal antibody (Product # PA1-46356) in Western Blot

Thu Oct 01 00:00:00 EDT 2020

Role of cysteine 288 in nucleophosmin cytoplasmic mutations: sensitization to toxicity induced by arsenic trioxide and bortezomib.

Leukemia

Huang M,Thomas D,Li MX,Feng W,Chan SM,Majeti R,Mitchell BS

PA1-46356 was used in immunocytochemistry to study the increased sensitivity to arsenic trioxide and bortezomib of cells bearing the nucleophosmin cysteine 288 mutation

Tue Oct 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: B23 antibody; MGC104254; mNPM antibody; mNPM1 antibody; NO38 antibody; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin 1; nucleophosmin/nucleoplasmin family, member 1; Numatrin; numatrin antibody; testicular tissue protein Li 128

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Gene Aliases: B23; NO38; NPM; NPM1

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UniProt ID: (Human) P06748, (Mouse) Q61937

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Entrez Gene ID: (Human) 4869, (Mouse) 18148

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Function(s)

transcription coactivator activity RNA binding protein kinase inhibitor activity protein binding protein kinase binding Tat protein binding histone binding protein homodimerization activity ribosomal large subunit binding ribosomal small subunit binding poly(A) RNA binding protein heterodimerization activity NF-kappaB binding unfolded protein binding nucleic acid binding DNA binding phosphatidylinositol-3,4,5-trisphosphate binding rRNA binding enzyme binding protein N-terminus binding chaperone

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NPM1 (mutant) Polyclonal Antibody

NPM1 (mutant) Antibody (PA1-46356) in ICC/IF

Product Details

PA1-46356

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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