Invitrogen

NPR1 Polyclonal Antibody

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

7 Published Figures

8 References

View all (18) NPR1 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 11

NPR1 Antibody (PA5-29049) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1) and tissue extracts of Rat Adipose (Lane 2). The blot was probed with Anti-NPR1 Polyclonal Antibody (Product # PA5-29049, 1:1000 dilution) and detected by chemiluminescence using Goat anti Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 118 kDa band corresponding to NPR1 was detected in the cell line and tissue tested. An uncharacterized band was also observed at ~20kDa in th... View More

NPR1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

NPR1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

NPR1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with NPR1 siRNA and reduction of signal was observed in Western Blot using NPR1 Polyclonal Antibody (Product # PA5-29049). {KD}

Product Details

PA5-29049

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Rabbit, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 640 and 916 of Human NPR-A

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2546525

Product Specific Information

Recommended positive controls: HL-60 membrane extracts.

Predicted reactivity: Mouse (95%), Rat (95%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms. The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A and type B. Also see NPR3, which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides and BNP, respectively).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Combination of an Angiotensin II Receptor and a Neprilysin Inhibitor Attenuates Liver Fibrosis by Preventing Hepatic Stellate Cell Activation.

Biomedicines

Suzuki J,Kaji K,Nishimura N,Kubo T,Tomooka F,Shibamoto A,Iwai S,Tsuji Y,Fujinaga Y,Kitagawa K,Namisaki T,Akahane T,Yoshiji H

PA5-29049 was used in Western Blotting to assess the effects of SAC/VAL in carbon tetrachloride (CCl4)-induced murine liver fibrosis as well as the in vitro phenotypes of hepatic stellate cell (HSC).

Thu Apr 27 00:00:00 EDT 2023

VDR Regulates BNP Promoting Neurite Growth and Survival of Cochlear Spiral Ganglion Neurons through cGMP-PKG Signaling Pathway.

Cells

Zhang X,Zhou K,Tian K,Zhu Q,Liu W,Liu Z,An X,Tian C,Li Y,Lu F,Sun F,Zha D

Published figure using NPR1 polyclonal antibody (Product # PA5-29049) in Immunohistochemistry

Wed Nov 23 00:00:00 EST 2022

VDR Regulates BNP Promoting Neurite Growth and Survival of Cochlear Spiral Ganglion Neurons through cGMP-PKG Signaling Pathway.

Cells

Zhang X,Zhou K,Tian K,Zhu Q,Liu W,Liu Z,An X,Tian C,Li Y,Lu F,Sun F,Zha D

Published figure using NPR1 polyclonal antibody (Product # PA5-29049) in Immunohistochemistry

Wed Nov 23 00:00:00 EST 2022

Atrial Natriuretic Peptide Promotes Neurite Outgrowth and Survival of Cochlear Spiral Ganglion Neurons in vitro Through NPR-A/cGMP/PKG Signaling.

Frontiers in cell and developmental biology

Sun F,Zhou K,Tian KY,Zhang XY,Liu W,Wang J,Zhong CP,Qiu JH,Zha DJ

Published figure using NPR1 polyclonal antibody (Product # PA5-29049) in Immunohistochemistry

Sat Jul 17 00:00:00 EDT 2021

Natriuretic Peptides Regulate Prostate Cells Inflammatory Behavior: Potential Novel Anticancer Agents for Prostate Cancer.

Biomolecules

Mezzasoma L,Talesa VN,Costanzi E,Bellezza I

Published figure using NPR1 polyclonal antibody (Product # PA5-29049) in Western Blot

Tue May 25 00:00:00 EDT 2021

Natriuretic Peptides Regulate Prostate Cells Inflammatory Behavior: Potential Novel Anticancer Agents for Prostate Cancer.

Biomolecules

Mezzasoma L,Talesa VN,Costanzi E,Bellezza I

Published figure using NPR1 polyclonal antibody (Product # PA5-29049) in Western Blot

Tue May 25 00:00:00 EDT 2021

An approach using Caenorhabditis elegans screening novel targets to suppress tumour cell proliferation.

Cell proliferation

Mao YQ,Han SF,Zhang SL,Zhang ZY,Kong CY,Chen HL,Li ZM,Cai PR,Han B,Wang LS

Published figure using NPR1 polyclonal antibody (Product # PA5-29049) in Immunohistochemistry

Mon Jun 01 00:00:00 EDT 2020

An approach using Caenorhabditis elegans screening novel targets to suppress tumour cell proliferation.

Cell proliferation

Mao YQ,Han SF,Zhang SL,Zhang ZY,Kong CY,Chen HL,Li ZM,Cai PR,Han B,Wang LS

Published figure using NPR1 polyclonal antibody (Product # PA5-29049) in Immunohistochemistry

Mon Jun 01 00:00:00 EDT 2020

Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent Manner.

Neural plasticity

Sun F,Zhou K,Tian KY,Wang J,Qiu JH,Zha DJ

Published figure using NPR1 polyclonal antibody (Product # PA5-29049) in Immunohistochemistry

Wed Sep 29 00:00:00 EDT 2021

Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent Manner.

Neural plasticity

Sun F,Zhou K,Tian KY,Wang J,Qiu JH,Zha DJ

Published figure using NPR1 polyclonal antibody (Product # PA5-29049) in Immunohistochemistry

Wed Sep 29 00:00:00 EDT 2021

ANP and BNP Exert Anti-Inflammatory Action via NPR-1/cGMP Axis by Interfering with Canonical, Non-Canonical, and Alternative Routes of Inflammasome Activation in Human THP1 Cells.

International journal of molecular sciences

Mezzasoma L,Talesa VN,Romani R,Bellezza I

Published figure using NPR1 polyclonal antibody (Product # PA5-29049) in Western Blot

Tue Dec 22 00:00:00 EST 2020

ANP and BNP Exert Anti-Inflammatory Action via NPR-1/cGMP Axis by Interfering with Canonical, Non-Canonical, and Alternative Routes of Inflammasome Activation in Human THP1 Cells.

International journal of molecular sciences

Mezzasoma L,Talesa VN,Romani R,Bellezza I

Published figure using NPR1 polyclonal antibody (Product # PA5-29049) in Western Blot

Tue Dec 22 00:00:00 EST 2020

Bioinformatics

Protein Aliases: ANP-A; ANPR-A; atrial natriuretic peptide A-type receptor; Atrial natriuretic peptide receptor 1; atrial natriuretic peptide receptor A; Atrial natriuretic peptide receptor type A; atrionatriuretic peptide receptor A; GC-A; Guanylate cyclase A; natriuretic peptide A type receptor; natriuretic peptide receptor A; natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A); NPR-A; testicular tissue protein Li 20

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Gene Aliases: ANPa; ANPRA; Gca; GUC2A; GUCY2A; NPR-A; NPR1; NPRA

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UniProt ID: (Human) P16066, (Rat) P18910

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Entrez Gene ID: (Rabbit) 100343663, (Human) 4881, (Rat) 24603

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Function(s)

adenylate cyclase activity guanylate cyclase activity protein kinase activity ATP binding GTP binding G-protein coupled peptide receptor activity natriuretic peptide receptor activity peptide hormone binding protein kinase binding hormone binding protein binding guanylate cyclase

Process(es)

cGMP biosynthetic process protein phosphorylation cell surface receptor signaling pathway receptor guanylyl cyclase signaling pathway G-protein coupled receptor signaling pathway body fluid secretion regulation of blood pressure negative regulation of angiogenesis negative regulation of cell growth positive regulation of cGMP biosynthetic process intracellular signal transduction positive regulation of urine volume positive regulation of renal sodium excretion regulation of vasodilation dopamine metabolic process regulation of vascular permeability negative regulation of smooth muscle cell proliferation regulation of cardiac conduction regulation of blood vessel size

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NPR1 Polyclonal Antibody

NPR1 Antibody (PA5-29049) in WB

Product Details

PA5-29049

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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