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Invitrogen
NQO1 Monoclonal Antibody (A180)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 13
NQO1 Antibody (MA1-16672) in ICC/IF
Immunofluorescence analysis of NQO1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NQO1 Mouse Monoclonal Antibody (A180) (Product # MA1-16672) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a ... View More
Product Details
MA1-16672
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Non-human primate,
Rat
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
A180
Immunogen
Full length recombinant NQO1 (from human lung).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2298735
Product Specific Information
This antibody reacts weakly with mouse tissue.
Target Information
NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Altered expression of the protein has been seen in many tumors and is also associated with Alzheimer's disease (AD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Azoreductase; Diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase 4; diaphorase 4 (NADH/NADPH); diaphorase-4; dioxin-inducible 1; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase (quinone); NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase, quinone 1; NAD(P)H menadione oxidoreductase 1, dioxin inducible; NAD(P)H:menadione oxidoreductase; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NQ01; Phylloquinone reductase; QR1; Quinone reductase 1
Gene Aliases: AV001255; DHQU; DIA4; DTD; Nmo-1; Nmo1; NMOR1; NMORI; NQO1; Ox-1; Ox1; QR1
UniProt ID: (Human) P15559, (Mouse) Q64669, (Rat) P05982
Entrez Gene ID: (Dog) 610935, (Human) 1728, (Mouse) 18104, (Rat) 24314
regulation of cellular amino acid metabolic process
xenobiotic metabolic process
nitric oxide biosynthetic process
synaptic transmission, cholinergic
aging
response to nutrient
response to toxic substance
removal of superoxide radicals
response to estradiol
negative regulation of catalytic activity
positive regulation of neuron apoptotic process
response to ethanol
oxidation-reduction process
superoxide metabolic process
response to oxidative stress
response to organic cyclic compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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