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NR0B2 Antibody (CF806319) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR0B2 (RC206422, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NR0B2. Positive lysates LY402893 (100 µg) and LC402893 (20 µg) can be purchased separately from OriGene.
Product Details
CF806319
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI5F10
Immunogen
Full length human recombinant protein of human NR0B2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Nr0b2a; nuclear receptor SHP; Nuclear receptor subfamily 0 group B member 2; nuclear receptor subfamily 0, group B, member 2; Orphan nuclear receptor SHP; Small heterodimer partner
Gene Aliases: NR0B2; SHP; SHP1
UniProt ID: (Human) Q15466
Entrez Gene ID: (Human) 8431
DNA binding
steroid hormone receptor activity
transcription corepressor activity
protein binding
transcription factor binding
protein domain specific binding
protein complex binding
protein homodimerization activity
peroxisome proliferator activated receptor binding
retinoid X receptor binding
thyroid hormone receptor binding
C4 zinc finger nuclear receptor
DNA-binding transcription factor
zinc finger transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
Notch signaling pathway
cholesterol metabolic process
response to glucose
positive regulation of gene expression
negative regulation of gene expression
organ regeneration
positive regulation of insulin secretion
steroid hormone mediated signaling pathway
negative regulation of sequence-specific DNA binding transcription factor activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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