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OriGene
NR3C1 Monoclonal Antibody (OTI6B5), TrueMAB™
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
Product Details
CF806259
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI6B5
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-265 of human NR3C1 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858
Gene Aliases: GCCR; GCR; GCRST; GR; GRL; NR3C1
UniProt ID: (Human) P04150
Entrez Gene ID: (Human) 2908
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
RNA binding
glucocorticoid receptor activity
steroid binding
protein binding
zinc ion binding
protein complex binding
glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity
steroid hormone binding
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
apoptotic process
chromosome segregation
mitotic nuclear division
signal transduction
covalent chromatin modification
glucocorticoid receptor signaling pathway
glucocorticoid mediated signaling pathway
positive regulation of transcription from RNA polymerase II promoter
cell division
cellular response to steroid hormone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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