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NR3C2 Polyclonal Antibody

NR3C2 Antibody in Immunocytochemistry (ICC/IF)

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NR3C2 Antibody (21854-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using NR3C2 antibody 21854-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

NR3C2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NR3C2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

21854-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

ChIP assay (ChIP)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NR3C2 Fusion Protein Ag16410 (1-350 aa encoded by BC111758)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.53 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody, specifically reacts with the 107 kDa MCR protein.

Immunogen sequence: METKGYHSL PEGLDMERRW GQVSQAVERS SLGPTERTDE NNYMEIVNVS CVSGAIPNNS TQGSSKEKQE LLPCLQQDNN RPGILTSDIK TELESKELSA TVAESMGLYM DSVRDADYSY EQQNQQGSMS PAKIYQNVEQ LVKFYKGNGH RPSTLSCVNT PLRSFMSDSG SSVNGGVMRA IVKSPIMCHE KSPSVCSPLN MTSSVCSPAG INSVSSTTAS FGSFPVHSPI TQGTPLTCSP NVENRGSRSH SPAHASNVGS PLSSPLSSMK SSISSPPSHC SVKSPVSSPN NVTLRSSVSS PANINNSRCS VSSPSNTNNR STLSSPAAST VGSICSPVNN AFSYTASGTS A (1-350 aa encoded by BC111758)

Target Information

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Mineralocorticoids are a family of steroids, secreted by the adrenal cortex, necessary for the regulation of a number of metabolic processes including electrolyte regulation. These compounds exert their effect through their interaction with the mineralocorticoid receptor (MR) and that complex's subsequent association with DNA. Given the function of mineralocorticoids, it is not surprising to find that the kidney is a primary target organ for mineralocorticoids and that this organ has been shown to contain MR. The corresponding gene for the mineralocorticoid receptor is NR3C2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NR3C2 mediates oxidised low-density lipoprotein-induced human coronary endothelial cells dysfunction via modulation of NLRP3 inflammasome activation.

Autoimmunity

Chen X,Li W,Chang C

Fri Dec 01 00:00:00 EST 2023

NR3C2 mediates oxidised low-density lipoprotein-induced human coronary endothelial cells dysfunction via modulation of NLRP3 inflammasome activation.

Autoimmunity

Chen X,Li W,Chang C

Fri Dec 01 00:00:00 EST 2023

The mineralocorticoid receptor modulates timing and location of genomic binding by glucocorticoid receptor in response to synthetic glucocorticoids in keratinocytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Carceller-Zazo E,Sevilla LM,Pons-Alonso O,Chiner-Oms Á,Amazit L,An Vu T,Vitellius G,Viengchareun S,Comas I,Jaszczyszyn Y,Abella M,Alegre-Martí A,Estébanez-Perpiñá E,Lombès M,Pérez P

Sun Jan 01 00:00:00 EST 2023

Eplerenone Prevents Cardiac Fibrosis by Inhibiting Angiogenesis in Unilateral Urinary Obstruction Rats.

Journal of the renin-angiotensin-aldosterone system : JRAAS

Chang Y,Ben Y,Li H,Xiong Y,Chen G,Hao J,Ma X,Gao X,Qiang P,Shimosawa T,Wang X,Yang F,Xu Q

Tue Oct 04 00:00:00 EDT 2022

Eplerenone Prevents Cardiac Fibrosis by Inhibiting Angiogenesis in Unilateral Urinary Obstruction Rats.

Journal of the renin-angiotensin-aldosterone system : JRAAS

Chang Y,Ben Y,Li H,Xiong Y,Chen G,Hao J,Ma X,Gao X,Qiang P,Shimosawa T,Wang X,Yang F,Xu Q

Tue Oct 04 00:00:00 EDT 2022

High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction.

EMBO reports

Yuan Y,Miao Y,Ren T,Huang F,Qian L,Chen X,Zuo Y,Zhang HG,He J,Qiao C,Du Q,Wu Q,Zhang W,Zhu C,Xu Y,Wu D,Shi W,Jiang J,Xu G,Zheng H

Wed Jan 05 00:00:00 EST 2022

MicroRNA-155-5p Targets NR3C2 to Promote Malignant Progression of Clear Cell Renal Cell Carcinoma.

Kidney & blood pressure research

Yan C,Wang P,Zhao C,Yin G,Meng X,Li L,Cai S,Meng B

Tue May 17 00:00:00 EDT 2022

Melatonin prevents experimental central serous chorioretinopathy in rats.

Journal of pineal research

Yu S,Cui K,Wu P,Wu B,Lu X,Huang R,Tang X,Lin J,Yang B,Zhao J,He Q,Liang X,Xu Y

Mon Aug 01 00:00:00 EDT 2022

Esaxerenone inhibits the macrophage-to-myofibroblast transition through mineralocorticoid receptor/TGF-β1 pathway in mice induced with aldosterone.

Frontiers in immunology

Qiang P,Hao J,Yang F,Han Y,Chang Y,Xian Y,Xiong Y,Gao X,Liang L,Shimosawa T,Xu Q

Mon Sep 26 00:00:00 EDT 2022

Huoxue Jiedu Huayu Formula Alleviates Cell Pyroptosis in Contralateral Kidneys of 6-Month-Old UUO Rats through the NLRP3/Caspase-1/IL-1β Pathway.

Evidence-based complementary and alternative medicine : eCAM

Ma X,Qiang P,Chen G,Wang Z,Wang X,Xu Q

Mon Apr 25 00:00:00 EDT 2022

UUO induces lung fibrosis with macrophage-myofibroblast transition in rats.

International immunopharmacology

Yang F,Chang Y,Zhang C,Xiong Y,Wang X,Ma X,Wang Z,Li H,Shimosawa T,Pei L,Xu Q

Thu Apr 01 00:00:00 EDT 2021

UUO induces lung fibrosis with macrophage-myofibroblast transition in rats.

International immunopharmacology

Yang F,Chang Y,Zhang C,Xiong Y,Wang X,Ma X,Wang Z,Li H,Shimosawa T,Pei L,Xu Q

Thu Apr 01 00:00:00 EDT 2021

Long Non-Coding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT1) Promotes Hypertension by Modulating the Hsa-miR-124-3p/Nuclear Receptor Subfamily 3, Group C, Member 2 (NR3C2) and Hsa-miR-135a-5p/NR3C2 Axis.

Medical science monitor : international medical journal of experimental and clinical research

Luo L,Wang Y,Hu P,Wu J

Sun Mar 29 00:00:00 EDT 2020

Aldosterone from endometrial glands is benefit for human decidualization.

Cell death & disease

Li SY,Song Z,Yan YP,Li B,Song MJ,Liu YF,Yang ZS,Li MY,Liu AX,Quan S,Yang ZM

Thu Aug 13 00:00:00 EDT 2020

Aldosterone from endometrial glands is benefit for human decidualization.

Cell death & disease

Li SY,Song Z,Yan YP,Li B,Song MJ,Liu YF,Yang ZS,Li MY,Liu AX,Quan S,Yang ZM

Thu Aug 13 00:00:00 EDT 2020

Aldosterone from endometrial glands is benefit for human decidualization.

Cell death & disease

Li SY,Song Z,Yan YP,Li B,Song MJ,Liu YF,Yang ZS,Li MY,Liu AX,Quan S,Yang ZM

Thu Aug 13 00:00:00 EDT 2020

Molecular medicine reports

Hu D,Dong R,Zhang Y,Yang Y,Chen Z,Tang Y,Fu M,Xu X,Tu L

Tue Sep 01 00:00:00 EDT 2020

Molecular medicine reports

Hu D,Dong R,Zhang Y,Yang Y,Chen Z,Tang Y,Fu M,Xu X,Tu L

Tue Sep 01 00:00:00 EDT 2020

Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.

Behavioural brain research

Lian S,Xu B,Wang D,Wang L,Li W,Yao R,Ji H,Wang J,Guo J,Li S,Yang H

Fri Feb 01 00:00:00 EST 2019

Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.

Behavioural brain research

Lian S,Xu B,Wang D,Wang L,Li W,Yao R,Ji H,Wang J,Guo J,Li S,Yang H

Fri Feb 01 00:00:00 EST 2019

Response of the maternal hypothalamus to cold stress during late pregnancy in rats.

Brain research

Wang D,Xu B,Wang J,Wang H,Guo J,Ji H,Li S,Wu R,Yang H,Lian S

Fri Nov 01 00:00:00 EDT 2019

Identification of mineralocorticoid receptor target genes in the mouse hippocampus.

Journal of neuroendocrinology

van Weert LTCM,Buurstede JC,Sips HCM,Vettorazzi S,Mol IM,Hartmann J,Prekovic S,Zwart W,Schmidt MV,Roozendaal B,Tuckermann JP,Sarabdjitsingh RA,Meijer OC

Thu Aug 01 00:00:00 EDT 2019

Inhibitory Effects of Oxymatrine on Transdifferentiation of Neonatal Rat Cardiac Fibroblasts to Myofibroblasts Induced by Aldosterone via Keap1/Nrf2 Signaling Pathways In Vitro.

Medical science monitor : international medical journal of experimental and clinical research

Yang Y,Chen S,Tao L,Gan S,Luo H,Xu Y,Shen X

Sat Jul 20 00:00:00 EDT 2019

Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.

Behavioural brain research

Lian S,Wang D,Xu B,Guo W,Wang L,Li W,Ji H,Wang J,Kong F,Zhen L,Li S,Zhang L,Guo J,Yang H

Mon Jul 02 00:00:00 EDT 2018

Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.

Behavioural brain research

Lian S,Wang D,Xu B,Guo W,Wang L,Li W,Ji H,Wang J,Kong F,Zhen L,Li S,Zhang L,Guo J,Yang H

Mon Jul 02 00:00:00 EDT 2018

Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats.

Oncotarget

Lian S,Guo J,Wang L,Li W,Wang J,Ji H,Kong F,Xu B,Li S,Yang H

Fri Dec 29 00:00:00 EST 2017

Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats.

Oncotarget

Lian S,Guo J,Wang L,Li W,Wang J,Ji H,Kong F,Xu B,Li S,Yang H

Fri Dec 29 00:00:00 EST 2017

Bioinformatics

Protein Aliases: aldosterone receptor; Mineralocorticoid receptor; Mineralocorticoid receptor (aldosterone receptor); mineralocorticoid receptor 1; mineralocorticoid receptor 2; mineralocorticoid receptor delta; MR; Nuclear receptor subfamily 3 group C member 2; nuclear receptor subfamily 3, group C, member 2 variant 3

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Gene Aliases: MCR; MLR; MR; NR3C2; NR3C2VIT

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UniProt ID: (Human) P08235, (Rat) P22199

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Entrez Gene ID: (Human) 4306, (Mouse) 110784, (Rat) 25672

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Function(s)

transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity steroid binding protein binding zinc ion binding sequence-specific DNA binding DNA binding double-stranded DNA binding lipid binding mineralocorticoid receptor activity hormone binding protein homodimerization activity metal ion binding protein heterodimerization activity

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter cellular sodium ion homeostasis excretion mineralocorticoid receptor signaling pathway regulation of cell proliferation steroid hormone mediated signaling pathway transcription initiation from RNA polymerase II promoter signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NR3C2 Polyclonal Antibody

NR3C2 Antibody (21854-1-AP) in ICC/IF

Product Details

21854-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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