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NR5A1 Antibody (PA5-95802) in WB
Western blot analysis of NR5A1 in extracts of various cell lines. Samples were incubated with NR5A1 Polyclonal antibody (Product # PA5-95802) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-95802
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Steroidogenic factor-1 (SF-1/Steroidogenic factor-1 (SF-1/NR5A1)) (NP_0049502)
Conjugate
Form
Liquid
Concentration
0.51 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2807604
Product Specific Information
Immunogen sequence: MDYSYDEDLD ELCPVCGDKV SGYHYGLLTC ESCKGFFKRT VQNNKHYTCT ESQSCKIDKT QRKRCPFCRF QKCLTVGMRL EAVRADRMRG GRNKFGPMYK RDRALKQQKK AQIRANGFKL; Positive Samples: Mouse testis, Rat testis, Rat ovary; Cellular Location: Nucleus
Target Information
Steroidogenic factor-1 (SF-1) is an orphan nuclear receptor involved in organ development and differentiation of the endocrine system. SF-1 has been shown to be essential for fetal development of the hypothalamus, pituitary gland, gonads, and the adrenal gland. SF-1 has been shown to promote transcriptional activity of many steroidogenic enzymes from the cytochrome P450 family. SF-1 contains two activation function domains, designated AF1 and AF2, common to many nuclear steroid receptors which have been shown to be necessary for interaction with other nuclear receptors as well as coactivators and corepressors including steroid receptor coactivator-1 (SRC-1), glucocorticoid receptor interacting protein 1 (GRIP1), cAMP response element binding protein (CREB) binding protein (CBP) and silencing mediator of retinoid acid and thyroid hormone receptor (SMRT).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adrenal 4 binding protein; Adrenal 4 binding protein (AD4BP); Adrenal 4-binding protein; ELP; Embryonal long terminal repeat-binding protein; Embryonal LTR-binding protein; fushi tarazu 1 factor homolog; Fushi tarazu factor homolog 1; Fushi tarazu factor homolog 1 (FTZ1); nuclear receptor AdBP4; Nuclear receptor subfamily 5 group A member 1; Nuclear receptor subfamily 5 group A member 1 (NR5A1); nuclear receptor subfamily 5, group A, member 1; SF-1; Steroid hormone receptor Ad4BP; Steroid hydroxylase positive regulator; Steroidogenic factor 1; Steroidogenic factor 1 (SF1); steroidogenic factor 1 nuclear receptor; steroidogenic factor-1; STF-1; STF1
Gene Aliases: AD4BP; Ad4BP/SF-1; ELP; ELP-3; Ftz-F1; FTZ1; FTZF1; hSF-1; NR5A1; POF7; SF-1; SF1; SPGF8; SRXY3
UniProt ID: (Human) Q13285, (Mouse) P33242, (Rat) P50569
Entrez Gene ID: (Human) 2516, (Mouse) 26423, (Rat) 83826
transcription regulatory region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
DNA binding
chromatin binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
steroid hormone receptor activity
transcription coactivator activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
phospholipid binding
zinc ion binding
enzyme binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
lipid binding
sequence-specific DNA binding
metal ion binding
C4 zinc finger nuclear receptor
luteinization
transcription initiation from RNA polymerase II promoter
cell-cell signaling
primary sex determination
male gonad development
hormone-mediated signaling pathway
tissue development
multicellular organism aging
cell differentiation
adrenal gland development
intracellular receptor signaling pathway
hormone metabolic process
steroid hormone mediated signaling pathway
positive regulation of transcription from RNA polymerase II promoter
regulation of steroid biosynthetic process
maintenance of protein location in nucleus
positive regulation of male gonad development
negative regulation of female gonad development
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
reproductive process
positive regulation of transcription, DNA-templated
female gonad development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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