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FIGURE: 1 / 2
NSA2 Antibody (PA5-100860) in ICC/IF
Immunocytochemistry analysis of NSA2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NSA2 polyclonal antibody (Product # PA5-100860) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100860
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NSA2(Accession O95478), corresponding to amino acid residues Q53-S103.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850358
Product Specific Information
Antibody detects endogenous levels of total TINP1.
Target Information
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ94393; Hairy cell leukemia protein 1; L-name related protein; L-name-related protein 42; LNR42; NSA2 ribosome biogenesis homolog; Ribosome biogenesis protein NSA2 homolog; TGF beta-inducible nuclear protein; TGF beta-inducible nuclear protein 1; TGF-beta inducible nuclear protein; TGF-beta-inducible nuclear protein 1; YR-29
Gene Aliases: 5730427N09Rik; CDK105; HCL-G1; HCLG1; HUSSY-29; HUSSY29; LNR42; NSA2; Nsa2p; TINP1; Yr29
UniProt ID: (Human) O95478, (Mouse) Q9CR47
Entrez Gene ID: (Human) 10412, (Rat) 171456, (Mouse) 59050
Performance Guarantee
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