Proteintech

NSDHL Polyclonal Antibody

NSDHL Antibody in Immunocytochemistry (ICC/IF)

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NSDHL Antibody (15111-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 15111-1-AP (NSDHL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NSDHL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

15111-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,200

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NSDHL Fusion Protein Ag7097 (1-303 aa encoded by BC000245)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEPAVSEPM RDQVARTHLT EDTPKVNADI EKVNQNQAKR CTVIGGSGFL GQHMVEQLLA RGYAVNVFDI QQGFDNPQVR FFLGDLCSRQ DLYPALKGVN TVFHCASPPP SSNNKELFYR VNYIGTKNVI ETCKEAGVQK LILTSSASVI FEGVDIKNGT EDLPYAMKPI DYYTETKILQ ERAVLGANDP EKNFLTTAIR PHGIFGPRDP QLVPILIEAA RNGKMKFVIG NGKNLVDFTF VENVVHGHIL AAEQLSRDST LGGKAFHITN DEPIPFWTFL SRILTGLNYE APKYHIPYWV AYYL (1-303 aa encoded by BC000245)

Target Information

The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cancer cell international

Zhou Z,Tang J,Lu Y,Jia J,Luo T,Su K,Dai X,Zhang H,Liu O

Sat Feb 25 00:00:00 EST 2023

CPSF1 positively regulates NSDHL by alternative polyadenylation and promotes gastric cancer progression.

American journal of cancer research

Kang W,Yang Y,Chen C,Yu C

Thu Nov 17 00:00:00 EST 2022

Insm2 deficiency results in female infertility by disturbing steroid pathway and decreasing ovarian reserve in mice.

Cell cycle (Georgetown, Tex.)

Li ZM,Li YY,Fei CF,Zhou LQ

Tue Nov 01 00:00:00 EDT 2022

Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro.

Stem cells international

Li JQ,Jiang HJ,Su XY,Feng L,Zhan NZ,Li SS,Chen ZJ,Chang BH,Cheng PZ,Yang L,Pei GX

Tue Jan 25 00:00:00 EST 2022

Gigantol Improves Cholesterol Metabolism and Progesterone Biosynthesis in MA-10 Leydig Cells.

Current issues in molecular biology

Basque A,Nguyen HT,Touaibia M,Martin LJ

Thu Dec 23 00:00:00 EST 2021

Statins affect cancer cell plasticity with distinct consequences for tumor progression and metastasis.

Cell reports

Dorsch M,Kowalczyk M,Planque M,Heilmann G,Urban S,Dujardin P,Forster J,Ueffing K,Nothdurft S,Oeck S,Paul A,Liffers ST,Kaschani F,Kaiser M,Schramm A,Siveke JT,Winslow MM,Fendt SM,Nalbant P,Grüner BM

Tue Nov 23 00:00:00 EST 2021

Cholesterol Pathway Inhibition Induces TGF-β Signaling to Promote Basal Differentiation in Pancreatic Cancer.

Cancer cell

Gabitova-Cornell L,Surumbayeva A,Peri S,Franco-Barraza J,Restifo D,Weitz N,Ogier C,Goldman AR,Hartman TR,Francescone R,Tan Y,Nicolas E,Shah N,Handorf EA,Cai KQ,O'Reilly AM,Sloma I,Chiaverelli R,Moffitt RA,Khazak V,Fang CY,Golemis EA,Cukierman E,Astsaturov I

Mon Oct 12 00:00:00 EDT 2020

Statins Synergize with Hedgehog Pathway Inhibitors for Treatment of Medulloblastoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Gordon RE,Zhang L,Peri S,Kuo YM,Du F,Egleston BL,Ng JMY,Andrews AJ,Astsaturov I,Curran T,Yang ZJ

Thu Mar 15 00:00:00 EDT 2018

Statins Synergize with Hedgehog Pathway Inhibitors for Treatment of Medulloblastoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Gordon RE,Zhang L,Peri S,Kuo YM,Du F,Egleston BL,Ng JMY,Andrews AJ,Astsaturov I,Curran T,Yang ZJ

Thu Mar 15 00:00:00 EDT 2018

Statins Synergize with Hedgehog Pathway Inhibitors for Treatment of Medulloblastoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Gordon RE,Zhang L,Peri S,Kuo YM,Du F,Egleston BL,Ng JMY,Andrews AJ,Astsaturov I,Curran T,Yang ZJ

Thu Mar 15 00:00:00 EDT 2018

Statins Synergize with Hedgehog Pathway Inhibitors for Treatment of Medulloblastoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Gordon RE,Zhang L,Peri S,Kuo YM,Du F,Egleston BL,Ng JMY,Andrews AJ,Astsaturov I,Curran T,Yang ZJ

Thu Mar 15 00:00:00 EDT 2018

EGFR and RB1 as Dual Biomarkers in HPV-Negative Head and Neck Cancer.

Molecular cancer therapeutics

Beck TN,Georgopoulos R,Shagisultanova EI,Sarcu D,Handorf EA,Dubyk C,Lango MN,Ridge JA,Astsaturov I,Serebriiskii IG,Burtness BA,Mehra R,Golemis EA

Sat Oct 01 00:00:00 EDT 2016

Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXR.

Cell reports

Gabitova L,Restifo D,Gorin A,Manocha K,Handorf E,Yang DH,Cai KQ,Klein-Szanto AJ,Cunningham D,Kratz LE,Herman GE,Golemis EA,Astsaturov I

Tue Sep 22 00:00:00 EDT 2015

Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXR.

Cell reports

Gabitova L,Restifo D,Gorin A,Manocha K,Handorf E,Yang DH,Cai KQ,Klein-Szanto AJ,Cunningham D,Kratz LE,Herman GE,Golemis EA,Astsaturov I

Tue Sep 22 00:00:00 EDT 2015

Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXR.

Cell reports

Gabitova L,Restifo D,Gorin A,Manocha K,Handorf E,Yang DH,Cai KQ,Klein-Szanto AJ,Cunningham D,Kratz LE,Herman GE,Golemis EA,Astsaturov I

Tue Sep 22 00:00:00 EDT 2015

Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXR.

Cell reports

Gabitova L,Restifo D,Gorin A,Manocha K,Handorf E,Yang DH,Cai KQ,Klein-Szanto AJ,Cunningham D,Kratz LE,Herman GE,Golemis EA,Astsaturov I

Tue Sep 22 00:00:00 EDT 2015

Hidden disease susceptibility and sexual dimorphism in the heterozygous knockout of Cyp51 from cholesterol synthesis.

PloS one

Lewinska M,Juvan P,Perse M,Jeruc J,Kos S,Lorbek G,Urlep Z,Keber R,Horvat S,Rozman D

Tue Dec 15 00:00:00 EST 2015

Hidden disease susceptibility and sexual dimorphism in the heterozygous knockout of Cyp51 from cholesterol synthesis.

PloS one

Lewinska M,Juvan P,Perse M,Jeruc J,Kos S,Lorbek G,Urlep Z,Keber R,Horvat S,Rozman D

Tue Dec 15 00:00:00 EST 2015

Targeting C4-demethylating genes in the cholesterol pathway sensitizes cancer cells to EGF receptor inhibitors via increased EGF receptor degradation.

Cancer discovery

Sukhanova A,Gorin A,Serebriiskii IG,Gabitova L,Zheng H,Restifo D,Egleston BL,Cunningham D,Bagnyukova T,Liu H,Nikonova A,Adams GP,Zhou Y,Yang DH,Mehra R,Burtness B,Cai KQ,Klein-Szanto A,Kratz LE,Kelley RI,Weiner LM,Herman GE,Golemis EA,Astsaturov I

Tue Jan 01 00:00:00 EST 2013

Targeting C4-demethylating genes in the cholesterol pathway sensitizes cancer cells to EGF receptor inhibitors via increased EGF receptor degradation.

Cancer discovery

Tue Jan 01 00:00:00 EST 2013

Bioinformatics

Protein Aliases: bare patches; NAD(P)-dependent steroid dehydrogenase-like protein; Protein H105e3; short chain dehydrogenase/reductase family 31E, member 1; Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating; striated

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Gene Aliases: AI747449; Bpa; H105E3; NSDHL; SDR31E1; Str; XAP104

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UniProt ID: (Human) Q15738, (Mouse) Q9R1J0, (Rat) Q5PPL3

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Entrez Gene ID: (Human) 50814, (Mouse) 18194, (Rat) 309262

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Function(s)

C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity 3-beta-hydroxy-delta5-steroid dehydrogenase activity sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor molecular_function dehydratase

Process(es)

hair follicle development cholesterol biosynthetic process smoothened signaling pathway oxidation-reduction process labyrinthine layer blood vessel development lipid metabolic process steroid biosynthetic process steroid metabolic process cholesterol metabolic process sterol biosynthetic process biological_process

Performance Guarantee

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NSDHL Polyclonal Antibody

NSDHL Antibody (15111-1-AP) in ICC/IF

Product Details

15111-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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