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NSF Antibody (PA5-78095) in ICC/IF
NSF Polyclonal Antibody detects NSF protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: NSF protein stained by NSF Polyclonal Antibody (Product # PA5-78095) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta-3 Tubulin Polyclonal Antibody [GT1338]diluted at 1:500. Blue: Fluoroshield with DAPI .
Product Details
PA5-78095
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human NSF. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.27 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2736261
Product Specific Information
Positive Control: 293T, mouse brain, rat brain
Predicted Reactivity: Xenopus laevis (88%), Pig (97%), Chicken (91%), Bovine (99%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: N-ethylmaleimide sensitive factor; N-ethylmaleimide sensitive fusion protein; N-ethylmaleimide-sensitive factor; N-ethylmaleimide-sensitive factor-like protein; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; Protein SKD2; Suppressor of K(+) transport growth defect 2; Vesicle-fusing ATPase; Vesicular-fusion protein NSF
Gene Aliases: AI316878; AU020090; AU067812; Erg1; NSF; SKD2
UniProt ID: (Human) P46459, (Mouse) P46460, (Rat) Q9QUL6
Entrez Gene ID: (Human) 4905, (Mouse) 18195, (Rat) 60355
protein binding
ATP binding
ATPase activity
syntaxin-1 binding
Rab GTPase binding
protein kinase binding
PDZ domain binding
protein complex binding
ATPase activity, coupled
metal ion binding
nucleotide binding
protein C-terminus binding
hydrolase activity
syntaxin binding
D1 dopamine receptor binding
ATP-dependent protein binding
membrane traffic protein
positive regulation of receptor recycling
potassium ion transport
intracellular protein transport
exocytosis
ER to Golgi vesicle-mediated transport
retrograde vesicle-mediated transport, Golgi to ER
intra-Golgi vesicle-mediated transport
vesicle-mediated transport
regulation of exocytosis
Golgi to plasma membrane protein transport
plasma membrane fusion
positive regulation of protein catabolic process
COPII vesicle coating
Golgi vesicle docking
transport
metabolic process
protein transport
vesicle fusion
neurotransmitter secretion
membrane fusion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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