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NTF3 Antibody (500-P82BT-25UG) in ELISA
Sandwich ELISA: To detect hNT-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of NTF3 Polyclonal Antibody, Biotin (Product # 500-P82BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech NTF3 Polyclonal Antibody (Product # 500-P82G-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hNT-3.
Product Details
500-P82BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 27.2 kDa Recombinant Human NT-3
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930789
Product Specific Information
AA Sequence of recombinant protein: MYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human NT-3. Anti-Human NT-3-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hNT-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NT-3 (500-P82G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hNT-3.
Western Blot: To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hNT-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P82BT-1MG will be provided as 2 x 500 µg
Target Information
The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HDNF; Nerve growth factor 2; Neurotrophic factor; Neurotrophin-3; NGF-2; NT-3
Gene Aliases: HDNF; NGF-2; NGF2; NT-3; NT3; NTF3
UniProt ID: (Human) P20783
Entrez Gene ID: (Human) 4908
activation of MAPK activity
positive regulation of receptor internalization
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
cell-cell signaling
nervous system development
positive regulation of cell proliferation
positive regulation of cell migration
activation of protein kinase B activity
positive regulation of peptidyl-serine phosphorylation
myelination
regulation of apoptotic process
negative regulation of neuron apoptotic process
regulation of neuron differentiation
positive regulation of glial cell differentiation
neuron projection morphogenesis
positive regulation of peptidyl-tyrosine phosphorylation
negative regulation of peptidyl-tyrosine phosphorylation
modulation of synaptic transmission
positive chemotaxis
induction of positive chemotaxis
activation of GTPase activity
positive regulation of actin cytoskeleton reorganization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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