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NUP214 Antibody (A300-716A-T) in WB
Detection of human and mouse NUP214 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), and 293T (50µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP214 antibody A300-716A-M (lot A300-716A-M-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A300-716A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Between 1250 and 1300
Conjugate
Form
Liquid
Concentration
200 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 214 kDa nucleoporin; CAN protein, putative oncogene; Nuclear pore complex protein Nup214; nucleoporin 214kDa; Nucleoporin Nup214; Protein CAN
Gene Aliases: CAIN; CAN; KIAA0023; NUP214
UniProt ID: (Human) P35658
Entrez Gene ID: (Human) 8021
RNA export from nucleus
mRNA export from nucleus
tRNA export from nucleus
protein import into nucleus
protein export from nucleus
mitotic nuclear envelope disassembly
regulation of glucose transport
viral process
protein sumoylation
viral transcription
gene silencing by RNA
regulation of mRNA stability
regulation of cell cycle
intracellular transport of virus
regulation of cellular response to heat
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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