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NUP43 Antibody (CF807558) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUP43 (RC209014, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NUP43. Positive lysates LY404768 (100 µg) and LC404768 (20 µg) can be purchased separately from OriGene.
Product Details
CF807558
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI4D3
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-253 of human NUP43 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: nucleoporin 43kDa; Nucleoporin Nup43; Nup107-160 subcomplex subunit Nup43; p42
Gene Aliases: bA350J20.1; NUP43; p42
UniProt ID: (Human) Q8NFH3
Entrez Gene ID: (Human) 348995
mRNA export from nucleus
tRNA export from nucleus
sister chromatid cohesion
mitotic nuclear division
mitotic nuclear envelope disassembly
regulation of glucose transport
protein transport
viral process
protein sumoylation
viral transcription
gene silencing by RNA
cell division
intracellular transport of virus
regulation of cellular response to heat
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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