Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 7
NUP62 Antibody (PA5-21435) in ICC/IF
Immunofluorescence analysis of NUP62 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NUP62 Polyclonal Antibody (Product # PA5-21435) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Product # A... View More
Product Details
PA5-21435
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 294 and 522 of nucleoporin p62 (Uniprot ID#P37198)
Conjugate
Form
Liquid
Concentration
0.19 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11153933
Product Specific Information
Recommended positive controls: A431, H1299, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2.
Predicted reactivity: Mouse (95%), Rat (95%), Xenopus laevis (89%), Rhesus Monkey (99%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SDS3 is a subunit of the histone deacetylase.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 62 kDa nucleoporin; DKFZp547L134; FLJ20822; FLJ43869; MGC841; nuclear pore complex 1; nuclear pore glycoprotein 62; Nuclear pore glycoprotein p62; nucleoporin 62kD; nucleoporin 62kDa; Nucleoporin Nup62; nucleoporin p62
Gene Aliases: AA589433; AI426861; AI790512; AU045898; D7Ertd649e; IBSN; Np62; NUP62; Nupc1; p62; SNDI
UniProt ID: (Human) P37198, (Rat) P17955, (Mouse) Q63850
Entrez Gene ID: (Human) 23636, (Rat) 65274, (Mouse) 18226
chromatin binding
nucleocytoplasmic transporter activity
protein binding
phospholipid binding
structural constituent of nuclear pore
kinesin binding
receptor signaling complex scaffold activity
Hsp70 protein binding
SH2 domain binding
ubiquitin binding
thyroid hormone receptor binding
PTB domain binding
Hsp90 protein binding
transporter
transcription, DNA-templated
RNA export from nucleus
mRNA export from nucleus
tRNA export from nucleus
protein import into nucleus
mitotic nuclear division
mitotic nuclear envelope disassembly
cell surface receptor signaling pathway
spermatogenesis
cell aging
cell death
negative regulation of cell proliferation
hormone-mediated signaling pathway
regulation of signal transduction
regulation of glucose transport
viral process
protein sumoylation
viral transcription
gene silencing by RNA
negative regulation of epidermal growth factor receptor signaling pathway
regulation of protein import into nucleus
negative regulation of apoptotic process
negative regulation of programmed cell death
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of MAP kinase activity
positive regulation of epidermal growth factor receptor signaling pathway
positive regulation of transcription, DNA-templated
regulation of Ras protein signal transduction
negative regulation of Ras protein signal transduction
protein heterotrimerization
ribonucleoprotein complex export from nucleus
intracellular transport of virus
regulation of cellular response to heat
protein transport
mRNA transport
transport
cell migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support