Invitrogen

NUP62 Monoclonal Antibody (RL31)

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NUP62 Antibody (MA1-073) in WB

Western blot analysis of Nucleoporin p62 was performed by loading whole cell lysates (20 µg) of the indicated samples and 5 µL of Thermo Scientific PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% milk in TBST for 1 hour at room temperature. Nucleoporin p62 was detected at ~62 kD in samples using a Nucleoporin p62 (RL31) monoclonal antibody (Product # MA1-073) at a d... View More

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Product Details

MA1-073

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

Immunocytochemistry (ICC/IF)

1:50

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rat

Published species

Not Applicable

Host/Isotype

Armenian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

RL31

Immunogen

O-linked glycoprotein-enriched fraction from rat liver nuclear envelopes raised in Armenian hamsters.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2-7.4, with 30% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325413

Product Specific Information

MA1-073 detects the nucleoporin p62 O-linked glycoprotein in the p62 complex from rat, human and non-human primate tissues.

MA1-073 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a single ~62 kDa protein representing nucleoporin p62 in rat liver nuclear envelope fractions. Immunofluorescence staining of p62 in rat liver with MA1-073 results in staining of both the nucleoplasmic and cytoplasmic surfaces of the nuclear pore complex (NPC).

The MA1-073 immunogen is O-linked glycoprotein-enriched fraction from rat liver nuclear envelopes raised in Armenian hamsters.

Target Information

SDS3 is a subunit of the histone deacetylase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Preferentially localized dynein and perinuclear dynactin associate with nuclear pore complex proteins to mediate genomic union during mammalian fertilization.

Journal of cell science

Payne C,Rawe V,Ramalho-Santos J,Simerly C,Schatten G

MA1-073 was used in western blot and immunocytochemistry to investigate the role of dynein and perinuclear dynactin in mammalian fertilization.

Mon Dec 01 00:00:00 EST 2003

Interaction of the transcription factor Sp1 with the nuclear pore protein p62 requires the C-terminal domain of p62.

Journal of cellular biochemistry

Han I,Roos MD,Kudlow JE

MA1-073 was used in immunoprecipitation and western blot to investigate the interaction between Sp1 and p62

Thu Jan 01 00:00:00 EST 1998

Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex.

Molecular biology of the cell

Guan T,Müller S,Klier G,Panté N,Blevitt JM,Haner M,Paschal B,Aebi U,Gerace L

MA1-073 was used in western blot and immunocytochemistry to identify the structural components of nuclear pore p62 complex.

Wed Nov 01 00:00:00 EST 1995

A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.

The Journal of cell biology

Featherstone C,Darby MK,Gerace L

MA1-073 was used in immunohistochemistry and western blot to investigate the important role of nuclear pore complex in transporting protein and RNA

Sat Oct 01 00:00:00 EDT 1988

Bioinformatics

Protein Aliases: 62 kDa nucleoporin; DKFZp547L134; FLJ20822; FLJ43869; MGC841; nuclear pore complex 1; nuclear pore glycoprotein 62; Nuclear pore glycoprotein p62; nucleoporin 62kD; nucleoporin 62kDa; Nucleoporin Nup62; nucleoporin p62

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Gene Aliases: AA589433; AI426861; AI790512; AU045898; D7Ertd649e; IBSN; Np62; NUP62; Nupc1; p62; SNDI

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UniProt ID: (Human) P37198, (Rat) P17955, (Mouse) Q63850

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Entrez Gene ID: (Human) 23636, (Dog) 484370, (Rat) 65274, (Mouse) 18226

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Function(s)

chromatin binding nucleocytoplasmic transporter activity protein binding phospholipid binding structural constituent of nuclear pore kinesin binding receptor signaling complex scaffold activity Hsp70 protein binding SH2 domain binding ubiquitin binding thyroid hormone receptor binding PTB domain binding Hsp90 protein binding transporter

Process(es)

transcription, DNA-templated RNA export from nucleus mRNA export from nucleus tRNA export from nucleus protein import into nucleus mitotic nuclear division mitotic nuclear envelope disassembly cell surface receptor signaling pathway spermatogenesis cell aging cell death negative regulation of cell proliferation hormone-mediated signaling pathway regulation of signal transduction regulation of glucose transport viral process protein sumoylation viral transcription gene silencing by RNA negative regulation of epidermal growth factor receptor signaling pathway regulation of protein import into nucleus negative regulation of apoptotic process negative regulation of programmed cell death positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of MAP kinase activity positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription, DNA-templated regulation of Ras protein signal transduction negative regulation of Ras protein signal transduction protein heterotrimerization ribonucleoprotein complex export from nucleus intracellular transport of virus regulation of cellular response to heat protein transport mRNA transport transport cell migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NUP62 Monoclonal Antibody (RL31)

NUP62 Antibody (MA1-073) in WB

Product Details

MA1-073

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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