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FIGURE: 1 / 1
NUP98 Antibody (PA5-95227) in WB
Western blot analysis of NUP98 in Lane 1: rat liver tissue lysate (50 µg), Lane 2: mouse liver tissue lysate (50 µg), Lane 3: mouse lung tissue lysate (50 µg), Lane 4: A549 whole cell lysate (40 µg), Lane 5: SMMC whole cell lysate (40 µg). Samples were incubated with NUP98 polyclonal antibody (Product # PA5-95227).
Product Details
PA5-95227
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human NUP98 recombinant protein (Position: H549-F880).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.01mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807031
Product Specific Information
Human NUP98 shares 95% and 94% amino acid (aa) sequence identity with mouse and rat NUP98, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kDa nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kDa precursor protein. This cleavage results in the 98 kDa nucleoporin as well as a 96 kDa nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kDa nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromosome translocations in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15. 5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 98 kDa nucleoporin; GLFG-repeat containing nucleoporin; nuclear pore complex protein Nup98; Nuclear pore complex protein Nup98-Nup96; nucleoporin 98kD; nucleoporin 98kDa; nucleoporin Nup98; Nup98-Nup96; NUP98/PHF23 fusion 2 protein; OTTHUMP00000013954
Gene Aliases: 4732457F17; ADAR2; ADIR2; AI849286; NUP196; NUP96; NUP98
UniProt ID: (Human) P52948, (Rat) P49793, (Mouse) Q6PFD9
Entrez Gene ID: (Human) 4928, (Rat) 81738, (Mouse) 269966
protein import into nucleus, docking
posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery
DNA replication
RNA export from nucleus
mRNA export from nucleus
tRNA export from nucleus
nucleocytoplasmic transport
nuclear pore organization
sister chromatid cohesion
mitotic nuclear envelope disassembly
regulation of glucose transport
viral process
protein sumoylation
viral transcription
gene silencing by RNA
telomere tethering at nuclear periphery
nuclear pore complex assembly
intracellular transport of virus
regulation of cellular response to heat
transport
mRNA transport
protein transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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