Proteintech

NUSAP1 Polyclonal Antibody

NUSAP1 Antibody in Immunocytochemistry (ICC/IF)

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NUSAP1 Antibody (12024-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUSAP1 antibody (Product # 12024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

NUSAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NUSAP1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

12024-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NUSAP1 Fusion Protein Ag2654 (1-440 aa encoded by BC001308)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MIIPSLEEL DSLKYSDLQN LAKSLGLRAN LRATKLLKAL KGYIKHEARK GNENQDESQT SASSCDETEI QISNQEEAER QPLGHVTKTR RRCKTVRVDP DSQQNHSEIK ISNPTEFQNH EKQESQDLRA TAKVPSPPDE HQEAENAVSS GNRDSKVPSE GKKSLYTDES SKPGKNKRTA ITTPNFKKLH EAHFKEMESI DQYIERKKKH FEEHNSMNEL KQPINKGGVR TPVPPRGRLS VASTPISQRR SQGRSCGPAS QSTLGLKGSL KRSAISAAKT GVRFSAATKD NEHKRSLTKT PARKSAHVTV SGGTPKGEAV LGTHKLKTIT GNSAAVITPF KLTTEATQTP VSNKKPVFDL KASLSRPLNY EPHKGKLKPW GQSKENNYLN QHVNRINFYK KTYKQPHLQT KEEQRKKREQ ERKEKKAKVL GMRRGLILAE D (1-440 aa encoded by BC001308)

Target Information

Ubiquitin: exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. 60S ribosomal protein L40: component of the 60S subunit of the ribosome. Ribosomal protein L40 is essential for translation of a subset of cellular transcripts, and especially for cap-dependent translation of vesicular stomatitis virus mRNAs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LINC01393, a Novel Long Non-Coding RNA, Promotes the Cell Proliferation, Migration and Invasion through MiR-128-3p/NUSAP1 Axis in Glioblastoma.

International journal of molecular sciences

Li D,Hu J,Li S,Zhou C,Feng M,Li L,Gao Y,Chen X,Wu X,Cao Y,Hao B,Chen L

Mon Mar 20 00:00:00 EDT 2023

Knockdown of NUSAP1 inhibits cell proliferation and invasion through downregulation of TOP2A in human glioblastoma.

Cell cycle (Georgetown, Tex.)

Hu Y,Xue Z,Qiu C,Feng Z,Qi Q,Wang J,Jin W,Zhong Z,Liu X,Li W,Zhang Q,Huang B,Chen A,Wang J,Yang N,Zhou W

Thu Sep 01 00:00:00 EDT 2022

Knockdown of NUSAP1 inhibits cell proliferation and invasion through downregulation of TOP2A in human glioblastoma.

Cell cycle (Georgetown, Tex.)

Hu Y,Xue Z,Qiu C,Feng Z,Qi Q,Wang J,Jin W,Zhong Z,Liu X,Li W,Zhang Q,Huang B,Chen A,Wang J,Yang N,Zhou W

Thu Sep 01 00:00:00 EDT 2022

The mechanism of acentrosomal spindle assembly in human oocytes.

Science (New York, N.Y.)

Wu T,Dong J,Fu J,Kuang Y,Chen B,Gu H,Luo Y,Gu R,Zhang M,Li W,Dong X,Sun X,Sang Q,Wang L

Fri Nov 18 00:00:00 EST 2022

NUSAP1 Could be a Potential Target for Preventing NAFLD Progression to Liver Cancer.

Frontiers in pharmacology

Zeng T,Chen G,Qiao X,Chen H,Sun L,Ma Q,Li N,Wang J,Dai C,Xu F

Sat Jul 16 00:00:00 EDT 2022

NUSAP1 Could be a Potential Target for Preventing NAFLD Progression to Liver Cancer.

Frontiers in pharmacology

Zeng T,Chen G,Qiao X,Chen H,Sun L,Ma Q,Li N,Wang J,Dai C,Xu F

Sat Jul 16 00:00:00 EDT 2022

NUSAP1 Could be a Potential Target for Preventing NAFLD Progression to Liver Cancer.

Frontiers in pharmacology

Zeng T,Chen G,Qiao X,Chen H,Sun L,Ma Q,Li N,Wang J,Dai C,Xu F

Sat Jul 16 00:00:00 EDT 2022

NUSAP1 Could be a Potential Target for Preventing NAFLD Progression to Liver Cancer.

Frontiers in pharmacology

Zeng T,Chen G,Qiao X,Chen H,Sun L,Ma Q,Li N,Wang J,Dai C,Xu F

Sat Jul 16 00:00:00 EDT 2022

Identification and clinical validation of NUSAP1 as a novel prognostic biomarker in ovarian cancer.

BMC cancer

Gou R,Zheng M,Hu Y,Gao L,Wang S,Liu O,Li X,Zhu L,Liu J,Lin B

Thu Jun 23 00:00:00 EDT 2022

AMD3465 (hexahydrobromide) rescues the MG63 osteoblasts against the apoptosis induced by high glucose.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zhang Z,Wu H,Huang S,Chen L,Chen X,Zhang W,Liao L,Zhang X

Tue Jun 01 00:00:00 EDT 2021

Nucleolar and spindle‑associated protein 1 promotes non‑small cell lung cancer progression and serves as an effector of myocyte enhancer factor 2D.

Oncology reports

Ling B,Wei P,Xiao J,Cen B,Wei H,Feng X,Ye G,Li S,Zhang Z,Liang W,Huang S,Huang W

Mon Mar 01 00:00:00 EST 2021

Novel gene-specific translation mechanism of dysregulated, chronic inflammation reveals promising, multifaceted COVID-19 therapeutics.

bioRxiv : the preprint server for biology

Wang L,Muneer A,Xie L,Zhang F,Wu B,Mei L,Lenarcic EM,Feng EH,Song J,Xiong Y,Yu X,Wang C,Gheorghe C,Torralba K,Cook JG,Wan YY,Moorman NJ,Song H,Jin J,Chen X

Mon Nov 16 00:00:00 EST 2020

NUSAP1 Promotes Gastric Cancer Tumorigenesis and Progression by Stabilizing the YAP1 Protein.

Frontiers in oncology

Guo H,Zou J,Zhou L,Zhong M,He Y,Huang S,Chen J,Li J,Xiong J,Fang Z,Xiang X

Fri Apr 09 00:00:00 EDT 2021

NUSAP1 Promotes Gastric Cancer Tumorigenesis and Progression by Stabilizing the YAP1 Protein.

Frontiers in oncology

Guo H,Zou J,Zhou L,Zhong M,He Y,Huang S,Chen J,Li J,Xiong J,Fang Z,Xiang X

Fri Apr 09 00:00:00 EDT 2021

NUSAP1 Promotes Gastric Cancer Tumorigenesis and Progression by Stabilizing the YAP1 Protein.

Frontiers in oncology

Guo H,Zou J,Zhou L,Zhong M,He Y,Huang S,Chen J,Li J,Xiong J,Fang Z,Xiang X

Fri Apr 09 00:00:00 EDT 2021

Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.

The Journal of experimental medicine

Wang Y,Song W,Wang J,Wang T,Xiong X,Qi Z,Fu W,Yang X,Chen YG

Mon Feb 03 00:00:00 EST 2020

Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.

The Journal of experimental medicine

Wang Y,Song W,Wang J,Wang T,Xiong X,Qi Z,Fu W,Yang X,Chen YG

Mon Feb 03 00:00:00 EST 2020

Nucleolar and Spindle Associated Protein 1 (NUSAP1) Promotes Bladder Cancer Progression Through the TGF-β Signaling Pathway.

OncoTargets and therapy

Gao S,Yin H,Tong H,Zhan K,Yang G,Hossain MA,Li T,Gou X,He W

Wed Apr 13 00:00:00 EDT 2022

RepoMan stimulates the chromosome-dependent pathway of microtubule assembly.

Cell cycle (Georgetown, Tex.)

De Munter S,Van der Hoeven G,Bollen M

Sun Nov 01 00:00:00 EDT 2020

Downregulation of nucleolar and spindle-associated protein 1 expression suppresses liver cancer cell function.

Experimental and therapeutic medicine

Wang Y,Ju L,Xiao F,Liu H,Luo X,Chen L,Lu Z,Bian Z

Mon Apr 01 00:00:00 EDT 2019

Downregulation of nucleolar and spindle-associated protein 1 expression suppresses liver cancer cell function.

Experimental and therapeutic medicine

Wang Y,Ju L,Xiao F,Liu H,Luo X,Chen L,Lu Z,Bian Z

Mon Apr 01 00:00:00 EDT 2019

Nucleolar spindle-associated protein 1 promotes tumorigenesis and predicts poor prognosis in human esophageal squamous cell carcinoma.

Journal of cellular biochemistry

Guan C,Liu Z,Lu C,Xiao M,Shi H,Ni R,Bian Z

Mon Jul 01 00:00:00 EDT 2019

Nucleolar spindle-associated protein 1 promotes tumorigenesis and predicts poor prognosis in human esophageal squamous cell carcinoma.

Journal of cellular biochemistry

Guan C,Liu Z,Lu C,Xiao M,Shi H,Ni R,Bian Z

Mon Jul 01 00:00:00 EDT 2019

Hepatitis B virus X protein enhances hepatocarcinogenesis by depressing the targeting of NUSAP1 mRNA by miR-18b.

Cancer biology & medicine

Yang Z,Li J,Feng G,Wang Y,Yang G,Liu Y,Zhang S,Feng J,Zhang X

Wed May 01 00:00:00 EDT 2019

Knockdown of SP1/Syncytin1 axis inhibits the proliferation and metastasis through the AKT and ERK1/2 signaling pathways in non-small cell lung cancer.

Cancer medicine

Li X,Fu Y,Xia X,Zhang X,Xiao K,Zhuang X,Zhang Y

Sun Sep 01 00:00:00 EDT 2019

microRNA 193a-5p Regulates Levels of Nucleolar- and Spindle-Associated Protein 1 to Suppress Hepatocarcinogenesis.

Gastroenterology

Roy S,Hooiveld GJ,Seehawer M,Caruso S,Heinzmann F,Schneider AT,Frank AK,Cardenas DV,Sonntag R,Luedde M,Trautwein C,Stein I,Pikarsky E,Loosen S,Tacke F,Ringelhan M,Avsaroglu SK,Goga A,Buendia MA,Vucur M,Heikenwalder M,Zucman-Rossi J,Zender L,Roderburg C,Luedde T

Sat Dec 01 00:00:00 EST 2018

Nucleolar and spindle-associated protein 1 is a tumor grade correlated prognosis marker for glioma patients.

CNS neuroscience & therapeutics

Zhu T,Xie P,Gao YF,Huang MS,Li X,Zhang W,Zhou HH,Liu ZQ

Thu Mar 01 00:00:00 EST 2018

Nucleolar and spindle-associated protein 1 is a tumor grade correlated prognosis marker for glioma patients.

CNS neuroscience & therapeutics

Zhu T,Xie P,Gao YF,Huang MS,Li X,Zhang W,Zhou HH,Liu ZQ

Thu Mar 01 00:00:00 EST 2018

High NUSAP1 expression predicts poor prognosis in colon cancer.

Pathology, research and practice

Liu Z,Guan C,Lu C,Liu Y,Ni R,Xiao M,Bian Z

Sun Jul 01 00:00:00 EDT 2018

APLP2, RRM2, and PRC1: New Putative Markers for the Differential Diagnosis of Thyroid Follicular Lesions.

Thyroid : official journal of the American Thyroid Association

Castelblanco E,Zafon C,Maravall J,Gallel P,Martinez M,Capel I,Bella MR,Halperin I,Temprana J,Iglesias C,Puig-Domingo M,Robledo M,Matias-Guiu X,Mauricio D

Sun Jan 01 00:00:00 EST 2017

APLP2, RRM2, and PRC1: New Putative Markers for the Differential Diagnosis of Thyroid Follicular Lesions.

Thyroid : official journal of the American Thyroid Association

Castelblanco E,Zafon C,Maravall J,Gallel P,Martinez M,Capel I,Bella MR,Halperin I,Temprana J,Iglesias C,Puig-Domingo M,Robledo M,Matias-Guiu X,Mauricio D

Sun Jan 01 00:00:00 EST 2017

Targeting PI3K/mTOR signaling exerts potent antitumor activity in pheochromocytoma in vivo.

Endocrine-related cancer

Lee M,Minaskan N,Wiedemann T,Irmler M,Beckers J,Yousefi BH,Kaissis G,Braren R,Laitinen I,Pellegata NS

Sun Jan 01 00:00:00 EST 2017

Targeting PI3K/mTOR signaling exerts potent antitumor activity in pheochromocytoma in vivo.

Endocrine-related cancer

Lee M,Minaskan N,Wiedemann T,Irmler M,Beckers J,Yousefi BH,Kaissis G,Braren R,Laitinen I,Pellegata NS

Sun Jan 01 00:00:00 EST 2017

NUSAP1 promotes invasion and metastasis of prostate cancer.

Oncotarget

Gordon CA,Gong X,Ganesh D,Brooks JD

Tue May 02 00:00:00 EDT 2017

NUSAP1 promotes invasion and metastasis of prostate cancer.

Oncotarget

Gordon CA,Gong X,Ganesh D,Brooks JD

Tue May 02 00:00:00 EDT 2017

The selective inhibition of protein phosphatase-1 results in mitotic catastrophe and impaired tumor growth.

Journal of cell science

Winkler C,De Munter S,Van Dessel N,Lesage B,Heroes E,Boens S,Beullens M,Van Eynde A,Bollen M

Tue Dec 15 00:00:00 EST 2015

The selective inhibition of protein phosphatase-1 results in mitotic catastrophe and impaired tumor growth.

Journal of cell science

Winkler C,De Munter S,Van Dessel N,Lesage B,Heroes E,Boens S,Beullens M,Van Eynde A,Bollen M

Tue Dec 15 00:00:00 EST 2015

NUSAP1 expression is upregulated by loss of RB1 in prostate cancer cells.

The Prostate

Gordon CA,Gulzar ZG,Brooks JD

Wed Apr 01 00:00:00 EDT 2015

NUSAP1 expression is upregulated by loss of RB1 in prostate cancer cells.

The Prostate

Gordon CA,Gulzar ZG,Brooks JD

Wed Apr 01 00:00:00 EDT 2015

Down-Regulation of Nucleolar and Spindle-Associated Protein 1 (NUSAP1) Expression Suppresses Tumor and Cell Proliferation and Enhances Anti-Tumor Effect of Paclitaxel in Oral Squamous Cell Carcinoma.

PloS one

Okamoto A,Higo M,Shiiba M,Nakashima D,Koyama T,Miyamoto I,Kasama H,Kasamatsu A,Ogawara K,Yokoe H,Tanzawa H,Uzawa K

Tue Jun 14 00:00:00 EDT 2016

Down-Regulation of Nucleolar and Spindle-Associated Protein 1 (NUSAP1) Expression Suppresses Tumor and Cell Proliferation and Enhances Anti-Tumor Effect of Paclitaxel in Oral Squamous Cell Carcinoma.

PloS one

Okamoto A,Higo M,Shiiba M,Nakashima D,Koyama T,Miyamoto I,Kasama H,Kasamatsu A,Ogawara K,Yokoe H,Tanzawa H,Uzawa K

Tue Jun 14 00:00:00 EDT 2016

Down-Regulation of Nucleolar and Spindle-Associated Protein 1 (NUSAP1) Expression Suppresses Tumor and Cell Proliferation and Enhances Anti-Tumor Effect of Paclitaxel in Oral Squamous Cell Carcinoma.

PloS one

Okamoto A,Higo M,Shiiba M,Nakashima D,Koyama T,Miyamoto I,Kasama H,Kasamatsu A,Ogawara K,Yokoe H,Tanzawa H,Uzawa K

Tue Jun 14 00:00:00 EDT 2016

Down-Regulation of Nucleolar and Spindle-Associated Protein 1 (NUSAP1) Expression Suppresses Tumor and Cell Proliferation and Enhances Anti-Tumor Effect of Paclitaxel in Oral Squamous Cell Carcinoma.

PloS one

Okamoto A,Higo M,Shiiba M,Nakashima D,Koyama T,Miyamoto I,Kasama H,Kasamatsu A,Ogawara K,Yokoe H,Tanzawa H,Uzawa K

Tue Jun 14 00:00:00 EDT 2016

High Levels of Nucleolar Spindle-Associated Protein and Reduced Levels of BRCA1 Expression Predict Poor Prognosis in Triple-Negative Breast Cancer.

PloS one

Chen L,Yang L,Qiao F,Hu X,Li S,Yao L,Yang XL,Shao ZM

Wed Jun 08 00:00:00 EDT 2016

High Levels of Nucleolar Spindle-Associated Protein and Reduced Levels of BRCA1 Expression Predict Poor Prognosis in Triple-Negative Breast Cancer.

PloS one

Chen L,Yang L,Qiao F,Hu X,Li S,Yao L,Yang XL,Shao ZM

Wed Jun 08 00:00:00 EDT 2016

Enhanced protein degradation by branched ubiquitin chains.

Cell

Meyer HJ,Rape M

Thu May 08 00:00:00 EDT 2014

Enhanced protein degradation by branched ubiquitin chains.

Cell

Meyer HJ,Rape M

Thu May 08 00:00:00 EDT 2014

Microtubule-dependent regulation of mitotic protein degradation.

Molecular cell

Song L,Craney A,Rape M

Thu Jan 23 00:00:00 EST 2014

Microtubule-dependent regulation of mitotic protein degradation.

Molecular cell

Song L,Craney A,Rape M

Thu Jan 23 00:00:00 EST 2014

Transcriptome analysis of MENX-associated rat pituitary adenomas identifies novel molecular mechanisms involved in the pathogenesis of human pituitary gonadotroph adenomas.

Acta neuropathologica

Lee M,Marinoni I,Irmler M,Psaras T,Honegger JB,Beschorner R,Anastasov N,Beckers J,Theodoropoulou M,Roncaroli F,Pellegata NS

Mon Jul 01 00:00:00 EDT 2013

Transcriptome analysis of MENX-associated rat pituitary adenomas identifies novel molecular mechanisms involved in the pathogenesis of human pituitary gonadotroph adenomas.

Acta neuropathologica

Lee M,Marinoni I,Irmler M,Psaras T,Honegger JB,Beschorner R,Anastasov N,Beckers J,Theodoropoulou M,Roncaroli F,Pellegata NS

Mon Jul 01 00:00:00 EDT 2013

Transcriptome analysis of MENX-associated rat pituitary adenomas identifies novel molecular mechanisms involved in the pathogenesis of human pituitary gonadotroph adenomas.

Acta neuropathologica

Lee M,Marinoni I,Irmler M,Psaras T,Honegger JB,Beschorner R,Anastasov N,Beckers J,Theodoropoulou M,Roncaroli F,Pellegata NS

Mon Jul 01 00:00:00 EDT 2013

Increased expression of NuSAP in recurrent prostate cancer is mediated by E2F1.

Oncogene

Gulzar ZG,McKenney JK,Brooks JD

Thu Jan 03 00:00:00 EST 2013

Increased expression of NuSAP in recurrent prostate cancer is mediated by E2F1.

Oncogene

Gulzar ZG,McKenney JK,Brooks JD

Thu Jan 03 00:00:00 EST 2013

Regulated degradation of spindle assembly factors by the anaphase-promoting complex.

Molecular cell

Song L,Rape M

Fri May 14 00:00:00 EDT 2010

Regulated degradation of spindle assembly factors by the anaphase-promoting complex.

Molecular cell

Song L,Rape M

Fri May 14 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Nucleolar and spindle-associated protein 1; nucleolar protein ANKT; NuSAP

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Gene Aliases: 2610201A12Rik; AI481307; ANKT; AW547774; BB165529; BM-037; BM037; LNP; NUSAP; NUSAP1; PRO0310; PRO0310p1; Q0310; SAPL

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UniProt ID: (Human) Q9BXS6, (Mouse) Q9ERH4

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Entrez Gene ID: (Human) 51203, (Rat) 311336, (Mouse) 108907

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Function(s)

DNA binding protein binding microtubule binding poly(A) RNA binding

Process(es)

mitotic sister chromatid segregation mitotic cytokinesis mitotic chromosome condensation establishment of mitotic spindle localization positive regulation of mitotic nuclear division microtubule cytoskeleton organization cell cycle mitotic nuclear division cell division

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NUSAP1 Polyclonal Antibody

NUSAP1 Antibody (12024-1-AP) in ICC/IF

Product Details

12024-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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