Invitrogen

Nanog Polyclonal Antibody, PeproTech®

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Antibody Testing Data (3)

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Nanog Antibody (500-P236-1MG) in WB

Western Blot: To detect Human Nanog by Western Blot analysis Nanog Polyclonal Antibody (Product # 500-P236-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Nanog is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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Product Details

500-P236-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

View 13 publications 13 publications

ELISA (ELISA)

0.5-2.0 µg/mL

In vitro Assay (IV)

View 2 publications 2 publications

Immunostaining (IS)

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human

Published species

Amphibian, Bovine, Dog, Human, Mouse, Pig

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human Nanog

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929967

Product Specific Information

AA Sequence of recombinant protein: SVDPACPQSL PCFEASDCKE SSPMPVICGP EENYPSLQMS SAEMPHTETV SPLPSSMDLL IQDSPDSSTS PKGKQPTSAE NSVAKKEDKV PVKKQKTRTV FSSTQLCVLN DRFQRQKYLS LQQMQELSNI LNLSYKQVKT WFQNQRMKSK RWQKNNWPKN SNGVTQKASA PTYPSLYSSY HQGCLVNPTG NLPMWSNQTW NNSTWSNQTQ NIQSWSNHSW NTQTWCTQSW NNQAWNSPFY NCGEESLQSC MQFQPNSPAS DLEAALEAAG EGLNVIQQTT RYFSTPQTMD LFLNYSMNMQ PEDV.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Nanog. Anti-Human Nanog-specific antibody was purified by affinity chromatography employing an immobilized Human Nanog matrix.

Sandwich ELISA: To detect Human Nanog by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Nanog (500-P236Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Nanog.

Western Blot: To detect Human Nanog by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Nanog is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

APOE4 Affects Basal and NMDAR-Mediated Protein Synthesis in Neurons by Perturbing Calcium Homeostasis.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Ramakrishna S,Jhaveri V,Konings SC,Nawalpuri B,Chakraborty S,Holst B,Schmid B,Gouras GK,Freude KK,Muddashetty RS

500-P236 was used in Immunocytochemistry-immunoflourescence to show that the defective protein synthesis mediated by APOE4 is closely linked to the perturbation of calcium homeostasis caused by APOE4 in neurons.

Wed Oct 20 00:00:00 EDT 2021

p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation.

Stem cells international

Li N,Du Z,Li Y,Xu W,Yang Y,Peng H,Song T,Qin Q,Lei H,Hua J

Tue Jan 04 00:00:00 EST 2022

Derivation of porcine extraembryonic endoderm-like cells from blastocysts.

Cell proliferation

Li Y,Wu S,Yu Y,Zhang H,Wei R,Lv J,Cai M,Yang X,Zhang Y,Liu Z

500-P236 was used in Immunocytochemistry-immunofluorescence to represent promising cell sources for human regenerative medicine.

Wed Apr 01 00:00:00 EDT 2020

Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis.

Nature communications

Ramos-Ibeas P,Sang F,Zhu Q,Tang WWC,Withey S,Klisch D,Wood L,Loose M,Surani MA,Alberio R

Wed Jan 30 00:00:00 EST 2019

A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos.

BMC developmental biology

Canizo JR,Ynsaurralde Rivolta AE,Vazquez Echegaray C,Suvá M,Alberio V,Aller JF,Guberman AS,Salamone DF,Alberio RH,Alberio R

500-P236 was used in Immunofluorescence to show that SOX17 can be used as a reliable early marker of hypoblast in the bovine, and based on its expression profile we show that the hypoblast segregates in day 7 blastocysts.

Thu Jul 04 00:00:00 EDT 2019

Untargeted histone profiling during naive conversion uncovers conserved modification markers between mouse and human.

Scientific reports

De Clerck L,Taelman J,Popovic M,Willems S,Van der Jeught M,Heindryckx B,De Sutter P,Marks H,Deforce D,Dhaenens M

Thu Nov 21 00:00:00 EST 2019

OCT4/POU5F1 is required for NANOG expression in bovine blastocysts.

Proceedings of the National Academy of Sciences of the United States of America

Simmet K,Zakhartchenko V,Philippou-Massier J,Blum H,Klymiuk N,Wolf E

Tue Mar 13 00:00:00 EDT 2018

RAS Regulates the Transition from Naive to Primed Pluripotent Stem Cells.

Stem cell reports

Altshuler A,Verbuk M,Bhattacharya S,Abramovich I,Haklai R,Hanna JH,Kloog Y,Gottlieb E,Shalom-Feuerstein R

Tue Mar 13 00:00:00 EDT 2018

Activation of Hes1 and Msx1 in Transgenic Mouse Embryonic Stem Cells Increases Differentiation into Neural Crest Derivatives.

International journal of molecular sciences

Méndez-Maldonado K,Vega-López G,Caballero-Chacón S,Aybar MJ,Velasco I

500-P236 was used in Immunocytochemistry-immunoflourescence to study the role of Hes1 and Msx1 in mouse embryonic stem cells (mESCs) undergoing neural crest (NC) differentiation.

Thu Dec 13 00:00:00 EST 2018

Changes in chromosome territory position within the nucleus reflect alternations in gene expression related to embryonic lineage specification.

PloS one

Orsztynowicz M,Lechniak D,Pawlak P,Kociucka B,Kubickova S,Cernohorska H,Madeja ZE

500-P236 was used in Immunocytochemistry-immunoflourescence to study the role of chromosome organisation in gene expression regulation of lineage specific factors in mammalian embryos and describe interactions at key developmental stages.

Fri Sep 29 00:00:00 EDT 2017

Induced pluripotent stem cells (iPSCs) derived from a patient with frontotemporal dementia caused by a P301L mutation in microtubule-associated protein tau (MAPT).

Stem cell research

Rasmussen MA,Hjermind LE,Hasholt LF,Waldemar G,Nielsen JE,Clausen C,Hyttel P,Holst B

500-P236 was used in Immunocytochemistry-immunofluorescence to investigate mutations in frontotemporal dementia.

Fri Jan 01 00:00:00 EST 2016

Generation of an isogenic, gene-corrected control cell line of the spinocerebellar ataxia type 2 patient-derived iPSC line H266.

Stem cell research

Marthaler AG,Tubsuwan A,Schmid B,Poulsen UB,Engelbrecht AF,Mau-Holzmann UA,Hyttel P,Nielsen TT,Nielsen JE,Holst B

500-P236 was used in Immunocytochemistry to develop a gene corrected cell line that will provide an ideal control to model SCA2 by iPSC technology. Here bona fide induced pluripotent stem cell (iPSC) lines of SCA2 patients were generated to study a disease-specific phenotype and gene correction of the iPSC line H266 clone 10 was demonstrated where expanded CAG repeat of the ATXN2 gene was excahanged with with the normal length found in healthy alleles.

Fri Jan 01 00:00:00 EST 2016

Generation of a human induced pluripotent stem cell line via CRISPR-Cas9 mediated integration of a site-specific heterozygous mutation in CHMP2B.

Stem cell research

Zhang Y,Schmid B,Nielsen TT,Nielsen JE,Clausen C,Hyttel P,Holst B,Freude KK

500-P236 was used in Immunohistochemistry to investigate the effects of CHMP2B mutation on induced pluripotent stem cells in frontotemporal dementia models.

Fri Jul 01 00:00:00 EDT 2016

Generation of an isogenic, gene-corrected control cell line of the spinocerebellar ataxia type 2 patient-derived iPSC line H196.

Stem cell research

Marthaler AG,Schmid B,Tubsuwan A,Poulsen UB,Engelbrecht AF,Mau-Holzmann UA,Hyttel P,Nielsen JE,Nielsen TT,Holst B

500-P236 was used in Immunocytoochemistry to investigate modelling of spinocerebellar ataxia type 2 with an induced pluripotent stem cell model.

Fri Jan 01 00:00:00 EST 2016

Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells.

Scientific reports

Congras A,Barasc H,Canale-Tabet K,Plisson-Petit F,Delcros C,Feraud O,Oudrhiri N,Hadadi E,Griscelli F,Bennaceur-Griscelli A,Turhan A,Afanassieff M,Ferchaud S,Pinton A,Yerle-Bouissou M,Acloque H

Wed Jun 01 00:00:00 EDT 2016

Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells.

Scientific reports

Congras A,Barasc H,Canale-Tabet K,Plisson-Petit F,Delcros C,Feraud O,Oudrhiri N,Hadadi E,Griscelli F,Bennaceur-Griscelli A,Turhan A,Afanassieff M,Ferchaud S,Pinton A,Yerle-Bouissou M,Acloque H

Wed Jun 01 00:00:00 EDT 2016

RE1-silencing Transcription Factor (REST) Is Required for Nuclear Reprogramming by Inhibiting Transforming Growth Factor β Signaling Pathway.

The Journal of biological chemistry

Kong QR,Xie BT,Zhang H,Li JY,Huang TQ,Wei RY,Liu ZH

Fri Dec 30 00:00:00 EST 2016

RE1-silencing Transcription Factor (REST) Is Required for Nuclear Reprogramming by Inhibiting Transforming Growth Factor β Signaling Pathway.

The Journal of biological chemistry

Kong QR,Xie BT,Zhang H,Li JY,Huang TQ,Wei RY,Liu ZH

Fri Dec 30 00:00:00 EST 2016

Porcine Pluripotent Stem Cells Derived from IVF Embryos Contribute to Chimeric Development In Vivo.

PloS one

Xue B,Li Y,He Y,Wei R,Sun R,Yin Z,Bou G,Liu Z

500-P236 was used in Immunohistochemistry-immunofluorescence to developed a porcine pluripotent stem cell (pPSC) line which would be useful for porcine genetic engineering and investigating the molecular mechanism of pluripotency regulation in pigs.

Wed Aug 03 00:00:00 EDT 2016

Porcine Pluripotent Stem Cells Derived from IVF Embryos Contribute to Chimeric Development In Vivo.

PloS one

Xue B,Li Y,He Y,Wei R,Sun R,Yin Z,Bou G,Liu Z

Wed Aug 03 00:00:00 EDT 2016

A facile method to establish human induced pluripotent stem cells from adult blood cells under feeder-free and xeno-free culture conditions: a clinically compliant approach.

Stem cells translational medicine

Chou BK,Gu H,Gao Y,Dowey SN,Wang Y,Shi J,Li Y,Ye Z,Cheng T,Cheng L

500-P236 was used in Immunocytochemistry to derive human induced pluripotent stem cells from blood mononuclear cells under clinically compliant culture conditions.

Wed Apr 01 00:00:00 EDT 2015

Markers of Pluripotency in Human Amniotic Epithelial Cells and Their Differentiation to Progenitor of Cortical Neurons.

PloS one

García-Castro IL,García-López G,Ávila-González D,Flores-Herrera H,Molina-Hernández A,Portillo W,Ramón-Gallegos E,Díaz NF

500-P236 was used in Immunocytochemistry-immunoflourescence to demonstrate that human amniotic epithelial cells (hAEC) express naïve pluripotent markers (KLF4, REX1 and TFE3) as well as the cortical neuron phenotype after differentiation.

Tue Jun 28 00:00:00 EDT 2016

Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines.

PloS one

Cerbini T,Funahashi R,Luo Y,Liu C,Park K,Rao M,Malik N,Zou J

500-P236 was used in Immunocytochemistry-immunoflourescence to study how TALEN-mediated CLYBL engineering provides improved transgene expression and options for multiple genetic modification in human stem cells.

Wed Dec 23 00:00:00 EST 2015

Changes in sub-cellular localisation of trophoblast and inner cell mass specific transcription factors during bovine preimplantation development.

BMC developmental biology

Madeja ZE,Sosnowski J,Hryniewicz K,Warzych E,Pawlak P,Rozwadowska N,Plusa B,Lechniak D

500-P236 was used in Immunohistochemistry-wholemount to study the mechanisms underlying trophoblast (TE)/inner cell mass (ICM) segregation in cattle and study protein and mRNA distribution during the crucial stages of bovine development.

Tue Aug 13 00:00:00 EDT 2013

Dual inhibition of Src and GSK3 maintains mouse embryonic stem cells, whose differentiation is mechanically regulated by Src signaling.

Stem cells (Dayton, Ohio)

Shimizu T,Ueda J,Ho JC,Iwasaki K,Poellinger L,Harada I,Sawada Y

Sun Jul 01 00:00:00 EDT 2012

Human embryonic stem cells exhibit increased propensity to differentiate during the G1 phase prior to phosphorylation of retinoblastoma protein.

Stem cells (Dayton, Ohio)

Sela Y,Molotski N,Golan S,Itskovitz-Eldor J,Soen Y

Fri Jun 01 00:00:00 EDT 2012

Modulation of pluripotency in the porcine embryo and iPS cells.

PloS one

Rodríguez A,Allegrucci C,Alberio R

500-P236 was used in Immunocytochemistry to study signaling pathways involved in the formation of the porcine ICM and establish whether their modulation can be used to increase the developmental potential of pluripotent cells and suggest that small molecule inhibitors could be used to increase the homogeneity of induced pluripotent stem cell cultures.

Thu May 23 00:00:00 EDT 2013

Modulation of pluripotency in the porcine embryo and iPS cells.

PloS one

Rodríguez A,Allegrucci C,Alberio R

Thu May 23 00:00:00 EDT 2013

Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.

BMC veterinary research

Takemitsu H,Zhao D,Yamamoto I,Harada Y,Michishita M,Arai T

Fri Aug 31 00:00:00 EDT 2012

Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.

BMC veterinary research

Takemitsu H,Zhao D,Yamamoto I,Harada Y,Michishita M,Arai T

Fri Aug 31 00:00:00 EDT 2012

Epigenetic reprogramming of breast cancer cells with oocyte extracts.

Molecular cancer

Allegrucci C,Rushton MD,Dixon JE,Sottile V,Shah M,Kumari R,Watson S,Alberio R,Johnson AD

Thu Jan 13 00:00:00 EST 2011

Epigenetic reprogramming of breast cancer cells with oocyte extracts.

Molecular cancer

Allegrucci C,Rushton MD,Dixon JE,Sottile V,Shah M,Kumari R,Watson S,Alberio R,Johnson AD

Thu Jan 13 00:00:00 EST 2011

Derivation and characterization of canine embryonic stem cell lines with in vitro and in vivo differentiation potential.

Stem cells (Dayton, Ohio)

Vaags AK,Rosic-Kablar S,Gartley CJ,Zheng YZ,Chesney A,Villagómez DA,Kruth SA,Hough MR

Sun Feb 01 00:00:00 EST 2009

Expression of pluripotency-related genes during bovine inner cell mass explant culture.

Cloning and stem cells

Pant D,Keefer CL

Tue Sep 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48

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Gene Aliases: NANOG

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UniProt ID: (Human) Q9H9S0

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Entrez Gene ID: (Human) 79923

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding sequence-specific DNA binding homeodomain transcription factor

Process(es)

endodermal cell fate specification transcription, DNA-templated regulation of transcription, DNA-templated cell proliferation regulation of gene expression stem cell population maintenance cell differentiation somatic stem cell population maintenance regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter negative regulation of nucleic acid-templated transcription

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Nanog Polyclonal Antibody, PeproTech®

Nanog Antibody (500-P236-1MG) in WB

Product Details

500-P236-1MG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

In vitro Assay (IV)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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