Invitrogen

Nanog Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

9 Published Figures

22 References

View all (45) Nanog antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Nanog Antibody in Immunocytochemistry (ICC/IF)

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Nanog Antibody (PA1-097) in ICC/IF

Immunofluorescent analysis of Nanog using anti-Nanog polyclonal antibody (Product # PA1-097) shows specific expression in human embryonal carcinoma NTERA-2 cells (shown in green) but not in negative control HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Nanog (Product # PA1-097), at a dilution of 1:200 for at least ... View More

Nanog Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nanog Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Nanog Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Product Image Gallery

Nanog Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Nanog Polyclonal antibody (Product # PA1-097X) showed expression of Nanog in NTERA-2 but not in HeLa which is reported to be negative for Nanog expression. {RE}

Product Details

PA1-097

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

5 µg/mL

View 10 publications 10 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ChIP assay (ChIP)

1-3 µL

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full-length human recombinant protein expressed in bacteria

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539867

Product Specific Information

This antibody is not recommended for mouse testes tissue or human ovarian carcinoma in IHC applications.

Target Information

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Correction of DMD in human iPSC-derived cardiomyocytes by base-editing-induced exon skipping.

Molecular therapy. Methods & clinical development

Wang P,Li H,Zhu M,Han RY,Guo S,Han R

PA1-097 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to explore the feasibility of base-editing-mediated exon skipping as a therapeutic strategy for DMD using cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs).

Thu Mar 09 00:00:00 EST 2023

Correction of DMD in human iPSC-derived cardiomyocytes by base-editing-induced exon skipping.

Molecular therapy. Methods & clinical development

Wang P,Li H,Zhu M,Han RY,Guo S,Han R

Thu Mar 09 00:00:00 EST 2023

Glioblastoma Cells Counteract PARP Inhibition through Pro-Survival Induction of Lipid Droplets Synthesis and Utilization.

Cancers

Majuelos-Melguizo J,Rodríguez-Vargas JM,Martínez-López N,Delgado-Bellido D,García-Díaz Á,Yuste VJ,García-Macía M,López LM,Singh R,Oliver FJ

Published figure using Nanog polyclonal antibody (Product # PA1-097) in Western Blot

Sun Jan 30 00:00:00 EST 2022

Autophagy Enhances Longevity of Induced Pluripotent Stem Cell-Derived Endothelium via mTOR-Independent ULK1 Kinase.

Stem cells translational medicine

Hekman KE,Koss KM,Ivancic DZ,He C,Wertheim JA

PA1-097 was used in Immunocytochemistry to find that autophagy activity was stimulated during differentiation but stagnated in mature iPSC-ECs.

Fri Nov 18 00:00:00 EST 2022

Autophagy Enhances Longevity of Induced Pluripotent Stem Cell-Derived Endothelium via mTOR-Independent ULK1 Kinase.

Stem cells translational medicine

Hekman KE,Koss KM,Ivancic DZ,He C,Wertheim JA

PA1-097 was used in Immunocytochemistry to find that autophagy activity was stimulated during differentiation but stagnated in mature iPSC-ECs.

Fri Nov 18 00:00:00 EST 2022

Generation of two induced pluripotent stem cell (iPSC) lines (BBANTWi006-A, BBANTWi007-A) from Brugada syndrome patients carrying an SCN5A mutation.

Stem cell research

Simons E,Nijak A,Loeys B,Alaerts M

PA1-097 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to report two iPSC lines (BBANTWi006-A, BBANTWi007-A) of a brother and a sister carrying an SCN5A mutation (c.4813 + 3_4813 + 6dupGGGT) causing BrS.

Fri Apr 01 00:00:00 EDT 2022

Generation and validation of an iPSC line (BBANTWi008-A) from a Loeys-Dietz Syndrome type 3 patient.

Stem cell research

Velchev JD,Verstraeten A,Meester J,Ponsaerts P,Richer J,Alaerts M,Loeys B

PA1-097 was used in Immunocytochemistry-immunofluorescence to show that this iPSC line can be used to study and/or therapeutically target the cellular pathomechanisms of SMAD3-related LDS.

Sat Oct 01 00:00:00 EDT 2022

Uncovering the Metabolic and Stress Responses of Human Embryonic Stem Cells to FTH1 Gene Silencing.

Cells

Scaramuzzino L,Lucchino V,Scalise S,Lo Conte M,Zannino C,Sacco A,Biamonte F,Parrotta EI,Costanzo FS,Cuda G

PA1-097 was used in Immunocytochemistry, Western Blot to shed new light on how hESCs perform under oxidative stress, dissecting the molecular mechanisms through which Nrf2, in combination with PPP, counteracts oxidative injury triggered by FTH1 knockdown.

Wed Sep 15 00:00:00 EDT 2021

Uncovering the Metabolic and Stress Responses of Human Embryonic Stem Cells to FTH1 Gene Silencing.

Cells

Scaramuzzino L,Lucchino V,Scalise S,Lo Conte M,Zannino C,Sacco A,Biamonte F,Parrotta EI,Costanzo FS,Cuda G

Wed Sep 15 00:00:00 EDT 2021

Uncovering the Metabolic and Stress Responses of Human Embryonic Stem Cells to FTH1 Gene Silencing.

Cells

Scaramuzzino L,Lucchino V,Scalise S,Lo Conte M,Zannino C,Sacco A,Biamonte F,Parrotta EI,Costanzo FS,Cuda G

Wed Sep 15 00:00:00 EDT 2021

Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment.

Nature protocols

Tekel SJ,Brookhouser N,Standage-Beier K,Wang X,Brafman DA

PA1-097 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot, Flow Cytometry to facilitate the highly efficient single-base editing of human cells at precise genomic loci.

Thu Jul 01 00:00:00 EDT 2021

Mesenchymal stem cells combined with liraglutide relieve acute lung injury through apoptotic signaling restrained by PKA/β-catenin.

Stem cell research & therapy

Yang X,Ma X,Don O,Song Y,Chen X,Liu J,Qu J,Feng Y

PA1-097 was used in Immunohistochemistry-immunofluorescence to demonstrate that LPS attenuates the proliferation of the three MSCs and the expression of GLP-1R.

Tue May 19 00:00:00 EDT 2020

Establishment and characterization of induced pluripotent stem cells (iPSCs) from central nervous system lupus erythematosus.

Journal of cellular and molecular medicine

De Angelis MT,Santamaria G,Parrotta EI,Scalise S,Lo Conte M,Gasparini S,Ferlazzo E,Aguglia U,Ciampi C,Sgura A,Cuda G

Published figure using Nanog polyclonal antibody (Product # PA1-097) in Immunofluorescence

Fri Nov 01 00:00:00 EDT 2019

FACT Sets a Barrier for Cell Fate Reprogramming in Caenorhabditis elegans and Human Cells.

Developmental cell

Kolundzic E,Ofenbauer A,Bulut SI,Uyar B,Baytek G,Sommermeier A,Seelk S,He M,Hirsekorn A,Vucicevic D,Akalin A,Diecke S,Lacadie SA,Tursun B

PA1-097 was used in Immunohistochemistry to identify FACT as a cellular reprogramming barrier in Caenorhabditis elegans and humans, revealing an evolutionarily conserved mechanism for cell fate protection.

Mon Sep 10 00:00:00 EDT 2018

Directed reprogramming of comprehensively characterized dental pulp stem cells extracted from natal tooth.

Scientific reports

Pisal RV,Suchanek J,Siller R,Soukup T,Hrebikova H,Bezrouk A,Kunke D,Micuda S,Filip S,Sullivan G,Mokry J

Published figure using Nanog polyclonal antibody (Product # PA1-097) in Immunofluorescence

Wed Apr 18 00:00:00 EDT 2018

Directed reprogramming of comprehensively characterized dental pulp stem cells extracted from natal tooth.

Scientific reports

Pisal RV,Suchanek J,Siller R,Soukup T,Hrebikova H,Bezrouk A,Kunke D,Micuda S,Filip S,Sullivan G,Mokry J

Published figure using Nanog polyclonal antibody (Product # PA1-097) in Immunofluorescence

Wed Apr 18 00:00:00 EDT 2018

Short-term retinoic acid treatment sustains pluripotency and suppresses differentiation of human induced pluripotent stem cells.

Cell death & disease

De Angelis MT,Parrotta EI,Santamaria G,Cuda G

Published figure using Nanog polyclonal antibody (Product # PA1-097) in Immunofluorescence

Fri Jan 05 00:00:00 EST 2018

Short-term retinoic acid treatment sustains pluripotency and suppresses differentiation of human induced pluripotent stem cells.

Cell death & disease

De Angelis MT,Parrotta EI,Santamaria G,Cuda G

Published figure using Nanog polyclonal antibody (Product # PA1-097) in Immunofluorescence

Fri Jan 05 00:00:00 EST 2018

Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.

Stem cell research & therapy

Ventura Ferreira MS,Bienert M,Müller K,Rath B,Goecke T,Opländer C,Braunschweig T,Mela P,Brümmendorf TH,Beier F,Neuss S

PA1-097 was used in Immunocytochemistry-immunoflourescence to study how besides SOX2 expression, no other pluripotency features were observed in early and late passages of CV-MSC.

Mon Feb 05 00:00:00 EST 2018

Modeling Short QT Syndrome Using Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Journal of the American Heart Association

El-Battrawy I,Lan H,Cyganek L,Zhao Z,Li X,Buljubasic F,Lang S,Yücel G,Sattler K,Zimmermann WH,Utikal J,Wieland T,Ravens U,Borggrefe M,Zhou XB,Akin I

PA1-097 was used in Immunocytochemistry to establish a cellular model of short QT syndrome, using human-induced pluripotent stem cell-derived cardiomyocytes.

Sat Mar 24 00:00:00 EDT 2018

Mutations in Disordered Regions Can Cause Disease by Creating Dileucine Motifs.

Cell

Meyer K,Kirchner M,Uyar B,Cheng JY,Russo G,Hernandez-Miranda LR,Szymborska A,Zauber H,Rudolph IM,Willnow TE,Akalin A,Haucke V,Gerhardt H,Birchmeier C,Kühn R,Krauss M,Diecke S,Pascual JM,Selbach M

PA1-097 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to study how mutations in disordered cytosolic regions of three transmembrane proteins (GLUT1, ITPR1, and CACNA1H) lead to an increased clathrin binding.

Thu Sep 20 00:00:00 EDT 2018

Thymidylate synthase is functionally associated with ZEB1 and contributes to the epithelial-to-mesenchymal transition of cancer cells.

The Journal of pathology

Siddiqui A,Vazakidou ME,Schwab A,Napoli F,Fernandez-Molina C,Rapa I,Stemmler MP,Volante M,Brabletz T,Ceppi P

PA1-097 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to indicate that the TS enzyme is functionally linked with EMT and cancer differentiation, with several potential translational implications.

Thu Jun 01 00:00:00 EDT 2017

MicroRNAs-Proteomic Networks Characterizing Human Medulloblastoma-SLCs.

Stem cells international

Catanzaro G,Besharat ZM,Garg N,Ronci M,Pieroni L,Miele E,Mastronuzzi A,Carai A,Alfano V,Po A,Screpanti I,Locatelli F,Urbani A,Ferretti E

Published figure using Nanog polyclonal antibody (Product # PA1-097) in Western Blot

Tue Feb 16 00:00:00 EST 2016

Genome-wide analysis of HIF-2α chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions.

Scientific reports

Lee MC,Huang HJ,Chang TH,Huang HC,Hsieh SY,Chen YS,Chou WY,Chiang CH,Lai CH,Shiau CY

PA1097 was used in western blot to elucidate the role of HIF in pseudohypoxia

Mon Jul 04 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48

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Gene Aliases: NANOG

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UniProt ID: (Human) Q9H9S0

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Entrez Gene ID: (Human) 79923

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding sequence-specific DNA binding homeodomain transcription factor

Process(es)

endodermal cell fate specification transcription, DNA-templated regulation of transcription, DNA-templated cell proliferation regulation of gene expression stem cell population maintenance cell differentiation somatic stem cell population maintenance regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter negative regulation of nucleic acid-templated transcription

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Nanog Polyclonal Antibody

Nanog Antibody (PA1-097) in ICC/IF

Product Details

PA1-097

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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