Invitrogen

Nanog Monoclonal Antibody (eBioMLC-51), Alexa Fluor™ 488, eBioscience™

FIGURE: 1 / 80

Nanog Antibody (53-5761-80) in Flow

F9 cells were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with 0.06 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 488 (Product # 53-4321-80) (blue histogram) or 0.06 µg of Nanog Monoclonal Antibody, Alexa Fluor 488 (purple histogram). Total cells were used for analysis.

Nanog Antibody in Immunohistochemistry (IHC)

Nanog Antibody in Immunocytochemistry (ICC/IF)

Nanog Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

Nanog Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Nanog Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Nanog Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

Product Details

53-5761-80

Applications

Tested Dilution

Publications

Western Blot (WB)

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

10 µg/mL

View 31 publications 31 publications

Flow Cytometry (Flow)

0.06 µg/test

View 8 publications 8 publications

ChIP assay (ChIP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioMLC-51

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_763611

Product Specific Information

Description: The eBioMLC51 monoclonal antibody recognizes mouse Nanog. Nanog is a multidomain homeobox transcription factor that has been shown to maintain pluripotency of embryonic stem cells, independent of LIF/Stat3. Expression of Nanog in the mouse is specific to early embryos, the ICM of the blastocyst, embryonic stem (ES) cells, and embryonic germ (EG) cells. Nanog expression often overlaps, but is not identical to, that of Oct4. Nanog is downregulated upon cellular differentiation and loss of pluripotency, making it a suitable marker in determining the undifferentiated state of stem cells.

Nanog acts as a transcriptional activator and has two activation domains in the C-terminus, called CD2 and WR, and one activation domain in the N terminus. The CD2 domain is unique to Nanog, whereas the NK2 DNA binding domain is well conserved.

Immunoblotting using eBioMLC51 reveals a band at ~ 45 kDa in F9 (an embryonal carcinoma cell line) lysate, but not in lysate from the NIH3T3 cell line or mouse spleen.

Preliminary data using fluorochrome-conjugated eBioMLC51 suggests that it is essential to use the eBioscience Foxp3 Staining Buffer Set, Product # 00-5523-00, for intracellular staining of mouse Nanog.

Applications Reported: This eBioMLC-51 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunocytochemistry.

Applications Tested: This eBioMLC-51 antibody has been tested by intracellular staining followed by flow cytometric analysis of the F9 cell line using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One-step protocol for intracellular (nuclear) proteins. This antibody can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This eBioMLC-51 antibody has been tested by immunocytochemistry on fixed and permeablized F9 cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Klf4 methylated by Prmt1 restrains the commitment of primitive endoderm.

Nucleic acids research

Zuo ZY,Yang GH,Wang HY,Liu SY,Zhang YJ,Cai Y,Chen F,Dai H,Xiao Y,Cheng MB,Huang Y,Zhang Y

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Mon Feb 28 00:00:00 EST 2022

Pramef12 enhances reprogramming into naïve iPS cells.

Biochemistry and biophysics reports

Haraguchi D,Nakamura T

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Western Blot

Fri Jul 01 00:00:00 EDT 2022

STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity.

Nature communications

Ohishi H,Shimada S,Uchino S,Li J,Sato Y,Shintani M,Owada H,Ohkawa Y,Pertsinidis A,Yamamoto T,Kimura H,Ochiai H

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Western Blot

Tue Dec 20 00:00:00 EST 2022

A balanced Oct4 interactome is crucial for maintaining pluripotency.

Science advances

Han D,Wu G,Chen R,Drexler HCA,MacCarthy CM,Kim KP,Adachi K,Gerovska D,Mavrommatis L,Bedzhov I,Araúzo-Bravo MJ,Schöler HR

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Fri Feb 18 00:00:00 EST 2022

Transplanted organoids empower human preclinical assessment of drug candidate for the clinic.

Science advances

Westerling-Bui AD,Fast EM,Soare TW,Venkatachalan S,DeRan M,Fanelli AB,Kyrychenko S,Hoang H,Corriea GM,Zhang W,Yu M,Daniels M,Malojcic G,Pan-Zhou XR,Ledeboer MW,Harmange JC,Emani M,Tibbitts TT,Reilly JF,Mundel P

53-5761-80 was used in Sample Preparation (FC/FACS) to develop a novel approach for PD studies using transplanted, perfused human kidney organoids.

Fri Jul 08 00:00:00 EDT 2022

Cell Surface Mechanics Gate Embryonic Stem Cell Differentiation.

Cell stem cell

Bergert M,Lembo S,Sharma S,Russo L,Milovanović D,Gretarsson KH,Börmel M,Neveu PA,Hackett JA,Petsalaki E,Diz-Muñoz A

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2021

Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency.

Cell stem cell

Kinoshita M,Barber M,Mansfield W,Cui Y,Spindlow D,Stirparo GG,Dietmann S,Nichols J,Smith A

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Thu Mar 04 00:00:00 EST 2021

Cell-cell communication through FGF4 generates and maintains robust proportions of differentiated cell types in embryonic stem cells.

Development (Cambridge, England)

Raina D,Bahadori A,Stanoev A,Protzek M,Koseska A,Schröter C

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Mon Nov 01 00:00:00 EDT 2021

Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre.

Nature communications

Xu PF,Borges RM,Fillatre J,de Oliveira-Melo M,Cheng T,Thisse B,Thisse C

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunohistochemistry

Wed Jun 02 00:00:00 EDT 2021

The transcription factor E2A drives neural differentiation in pluripotent cells.

Development (Cambridge, England)

Rao C,Malaguti M,Mason JO,Lowell S

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Mon Jun 22 00:00:00 EDT 2020

Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells.

Science advances

Ochiai H,Hayashi T,Umeda M,Yoshimura M,Harada A,Shimizu Y,Nakano K,Saitoh N,Liu Z,Yamamoto T,Okamura T,Ohkawa Y,Kimura H,Nikaido I

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Mon Jun 01 00:00:00 EDT 2020

The transition from local to global patterns governs the differentiation of mouse blastocysts.

PloS one

Fischer SC,Corujo-Simon E,Lilao-Garzon J,Stelzer EHK,Muñoz-Descalzo S

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunohistochemistry

Fri Aug 07 00:00:00 EDT 2020

Distinct Molecular Trajectories Converge to Induce Naive Pluripotency.

Cell stem cell

Stuart HT,Stirparo GG,Lohoff T,Bates LE,Kinoshita M,Lim CY,Sousa EJ,Maskalenka K,Radzisheuskaya A,Malcolm AA,Alves MRP,Lloyd RL,Nestorowa S,Humphreys P,Mansfield W,Reik W,Bertone P,Nichols J,Göttgens B,Silva JCR

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Thu Sep 05 00:00:00 EDT 2019

Tox4 modulates cell fate reprogramming.

Journal of cell science

Vanheer L,Song J,De Geest N,Janiszewski A,Talon I,Provenzano C,Oh T,Chappell J,Pasque V

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Tue Oct 22 00:00:00 EDT 2019

Endogenous epitope-tagging of Tet1, Tet2 and Tet3 identifies TET2 as a naïve pluripotency marker.

Life science alliance

Pantier R,Tatar T,Colby D,Chambers I

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Tue Oct 01 00:00:00 EDT 2019

Glutamine independence is a selectable feature of pluripotent stem cells.

Nature metabolism

Vardhana SA,Arnold PK,Rosen BP,Chen Y,Carey BW,Huangfu D,Carmona Fontaine C,Thompson CB,Finley LWS

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Mon Jul 01 00:00:00 EDT 2019

Glutamine independence is a selectable feature of pluripotent stem cells.

Nature metabolism

Vardhana SA,Arnold PK,Rosen BP,Chen Y,Carey BW,Huangfu D,Carmona Fontaine C,Thompson CB,Finley LWS

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Mon Jul 01 00:00:00 EDT 2019

Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions.

Molecular systems biology

Yachie-Kinoshita A,Onishi K,Ostblom J,Langley MA,Posfai E,Rossant J,Zandstra PW

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Mon Jan 29 00:00:00 EST 2018

Four simple rules that are sufficient to generate the mammalian blastocyst.

PLoS biology

Nissen SB,Perera M,Gonzalez JM,Morgani SM,Jensen MH,Sneppen K,Brickman JM,Trusina A

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Sat Jul 01 00:00:00 EDT 2017

Dynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states.

eLife

Jang S,Choubey S,Furchtgott L,Zou LN,Doyle A,Menon V,Loew EB,Krostag AR,Martinez RA,Madisen L,Levi BP,Ramanathan S

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Wed Mar 15 00:00:00 EDT 2017

Dynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states.

eLife

Jang S,Choubey S,Furchtgott L,Zou LN,Doyle A,Menon V,Loew EB,Krostag AR,Martinez RA,Madisen L,Levi BP,Ramanathan S

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Wed Mar 15 00:00:00 EDT 2017

A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation.

eLife

Li MA,Amaral PP,Cheung P,Bergmann JH,Kinoshita M,Kalkan T,Ralser M,Robson S,von Meyenn F,Paramor M,Yang F,Chen C,Nichols J,Spector DL,Kouzarides T,He L,Smith A

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Fri Aug 18 00:00:00 EDT 2017

NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.

Nature

Murakami K,Günesdogan U,Zylicz JJ,Tang WWC,Sengupta R,Kobayashi T,Kim S,Butler R,Dietmann S,Surani MA

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in ChIP assay

Thu Jan 21 00:00:00 EST 2016

NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers.

Nature

Murakami K,Günesdogan U,Zylicz JJ,Tang WWC,Sengupta R,Kobayashi T,Kim S,Butler R,Dietmann S,Surani MA

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in ChIP assay

Thu Jan 21 00:00:00 EST 2016

Selection and dynamics of embryonic stem cell integration into early mouse embryos.

Development (Cambridge, England)

Alexandrova S,Kalkan T,Humphreys P,Riddell A,Scognamiglio R,Trumpp A,Nichols J

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Fri Jan 01 00:00:00 EST 2016

Selection and dynamics of embryonic stem cell integration into early mouse embryos.

Development (Cambridge, England)

Alexandrova S,Kalkan T,Humphreys P,Riddell A,Scognamiglio R,Trumpp A,Nichols J

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Fri Jan 01 00:00:00 EST 2016

Identification of a Hematopoietic Cell Dedifferentiation-Inducing Factor.

Journal of cellular physiology

Li Y,Adomat H,Guns ET,Hojabrpour P,Duronio V,Curran TA,Jalili RB,Jia W,Delwar Z,Zhang Y,Elizei SS,Ghahary A

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Wed Jun 01 00:00:00 EDT 2016

Identification of a Hematopoietic Cell Dedifferentiation-Inducing Factor.

Journal of cellular physiology

Li Y,Adomat H,Guns ET,Hojabrpour P,Duronio V,Curran TA,Jalili RB,Jia W,Delwar Z,Zhang Y,Elizei SS,Ghahary A

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Wed Jun 01 00:00:00 EDT 2016

Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency.

Stem cell reports

Martin Gonzalez J,Morgani SM,Bone RA,Bonderup K,Abelchian S,Brakebusch C,Brickman JM

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Tue Aug 09 00:00:00 EDT 2016

p38 (Mapk14/11) occupies a regulatory node governing entry into primitive endoderm differentiation during preimplantation mouse embryo development.

Open biology

Thamodaran V,Bruce AW

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Thu Sep 01 00:00:00 EDT 2016

FGF/MAPK signaling sets the switching threshold of a bistable circuit controlling cell fate decisions in embryonic stem cells.

Development (Cambridge, England)

Schröter C,Rué P,Mackenzie JP,Martinez Arias A

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2015

FGF/MAPK signaling sets the switching threshold of a bistable circuit controlling cell fate decisions in embryonic stem cells.

Development (Cambridge, England)

Schröter C,Rué P,Mackenzie JP,Martinez Arias A

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2015

Snai1 promotes ESC exit from the pluripotency by direct repression of self-renewal genes.

Stem cells (Dayton, Ohio)

Galvagni F,Lentucci C,Neri F,Dettori D,De Clemente C,Orlandini M,Anselmi F,Rapelli S,Grillo M,Borghi S,Oliviero S

53-5761 was used in Immunocytochemistry-immunoflourescence to suggest that Snai1 is a key factor that triggers embryonic stem cells exit from the pluripotency state and initiate their differentiation processes.

Sun Mar 01 00:00:00 EST 2015

Reprogramming Roadblocks Are System Dependent.

Stem cell reports

Chantzoura E,Skylaki S,Menendez S,Kim SI,Johnsson A,Linnarsson S,Woltjen K,Chambers I,Kaji K

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Western Blot

Tue Sep 08 00:00:00 EDT 2015

Intrinsic factors and the embryonic environment influence the formation of extragonadal teratomas during gestation.

BMC developmental biology

Economou C,Tsakiridis A,Wymeersch FJ,Gordon-Keylock S,Dewhurst RE,Fisher D,Medvinsky A,Smith AJ,Wilson V

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Fri Oct 09 00:00:00 EDT 2015

X chromosome reactivation dynamics reveal stages of reprogramming to pluripotency.

Cell

Pasque V,Tchieu J,Karnik R,Uyeda M,Sadhu Dimashkie A,Case D,Papp B,Bonora G,Patel S,Ho R,Schmidt R,McKee R,Sado T,Tada T,Meissner A,Plath K

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Thu Dec 18 00:00:00 EST 2014

Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells.

EMBO molecular medicine

Huijbers IJ,Bin Ali R,Pritchard C,Cozijnsen M,Kwon MC,Proost N,Song JY,de Vries H,Badhai J,Sutherland K,Krimpenfort P,Michalak EM,Jonkers J,Berns A

53-5761 was used in Flow cytometry/Cell sorting to adapt and improve the use of genetically engineered mouse models for validating cancer genes and drug targets.

Sat Feb 01 00:00:00 EST 2014

In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity.

PLoS biology

Gouti M,Tsakiridis A,Wymeersch FJ,Huang Y,Kleinjung J,Wilson V,Briscoe J

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Fri Aug 01 00:00:00 EDT 2014

Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors.

Development (Cambridge, England)

Tsakiridis A,Huang Y,Blin G,Skylaki S,Wymeersch F,Osorno R,Economou C,Karagianni E,Zhao S,Lowell S,Wilson V

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Sat Mar 01 00:00:00 EST 2014

Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors.

Development (Cambridge, England)

Tsakiridis A,Huang Y,Blin G,Skylaki S,Wymeersch F,Osorno R,Economou C,Karagianni E,Zhao S,Lowell S,Wilson V

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Sat Mar 01 00:00:00 EST 2014

Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst.

Development (Cambridge, England)

Le Bin GC,Muñoz-Descalzo S,Kurowski A,Leitch H,Lou X,Mansfield W,Etienne-Dumeau C,Grabole N,Mulas C,Niwa H,Hadjantonakis AK,Nichols J

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Sat Mar 01 00:00:00 EST 2014

Formation of a polarised primitive endoderm layer in embryoid bodies requires fgfr/erk signalling.

PloS one

Doughton G,Wei J,Tapon N,Welham MJ,Chalmers AD

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Wed Jan 14 00:00:00 EST 2015

AID stabilizes stem-cell phenotype by removing epigenetic memory of pluripotency genes.

Nature

Kumar R,DiMenna L,Schrode N,Liu TC,Franck P,Muñoz-Descalzo S,Hadjantonakis AK,Zarrin AA,Chaudhuri J,Elemento O,Evans T

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Thu Aug 01 00:00:00 EDT 2013

A defined Oct4 level governs cell state transitions of pluripotency entry and differentiation into all embryonic lineages.

Nature cell biology

Radzisheuskaya A,Chia Gle B,dos Santos RL,Theunissen TW,Castro LF,Nichols J,Silva JC

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Western Blot

Sat Jun 01 00:00:00 EDT 2013

A defined Oct4 level governs cell state transitions of pluripotency entry and differentiation into all embryonic lineages.

Nature cell biology

Radzisheuskaya A,Chia Gle B,dos Santos RL,Theunissen TW,Castro LF,Nichols J,Silva JC

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Western Blot

Sat Jun 01 00:00:00 EDT 2013

Long-range chromatin contacts in embryonic stem cells reveal a role for pluripotency factors and polycomb proteins in genome organization.

Cell stem cell

Denholtz M,Bonora G,Chronis C,Splinter E,de Laat W,Ernst J,Pellegrini M,Plath K

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunocytochemistry

Thu Nov 07 00:00:00 EST 2013

Tsix RNA and the germline factor, PRDM14, link X reactivation and stem cell reprogramming.

Molecular cell

Payer B,Rosenberg M,Yamaji M,Yabuta Y,Koyanagi-Aoi M,Hayashi K,Yamanaka S,Saitou M,Lee JT

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunohistochemistry

Thu Dec 26 00:00:00 EST 2013

Tsix RNA and the germline factor, PRDM14, link X reactivation and stem cell reprogramming.

Molecular cell

Payer B,Rosenberg M,Yamaji M,Yabuta Y,Koyanagi-Aoi M,Hayashi K,Yamanaka S,Saitou M,Lee JT

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunohistochemistry

Thu Dec 26 00:00:00 EST 2013

Totipotent embryonic stem cells arise in ground-state culture conditions.

Cell reports

Morgani SM,Canham MA,Nichols J,Sharov AA,Migueles RP,Ko MS,Brickman JM

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Flow Cytometry

Thu Jun 27 00:00:00 EDT 2013

Totipotent embryonic stem cells arise in ground-state culture conditions.

Cell reports

Morgani SM,Canham MA,Nichols J,Sharov AA,Migueles RP,Ko MS,Brickman JM

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Flow Cytometry

Thu Jun 27 00:00:00 EDT 2013

The transcriptional and epigenomic foundations of ground state pluripotency.

Cell

Marks H,Kalkan T,Menafra R,Denissov S,Jones K,Hofemeister H,Nichols J,Kranz A,Stewart AF,Smith A,Stunnenberg HG

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Flow Cytometry

Fri Apr 27 00:00:00 EDT 2012

The transcriptional and epigenomic foundations of ground state pluripotency.

Cell

Marks H,Kalkan T,Menafra R,Denissov S,Jones K,Hofemeister H,Nichols J,Kranz A,Stewart AF,Smith A,Stunnenberg HG

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Flow Cytometry

Fri Apr 27 00:00:00 EDT 2012

Correlations between the levels of Oct4 and Nanog as a signature for naïve pluripotency in mouse embryonic stem cells.

Stem cells (Dayton, Ohio)

Muñoz Descalzo S,Rué P,Garcia-Ojalvo J,Martinez Arias A

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Sat Dec 01 00:00:00 EST 2012

Histone variant macroH2A marks embryonic differentiation in vivo and acts as an epigenetic barrier to induced pluripotency.

Journal of cell science

Pasque V,Radzisheuskaya A,Gillich A,Halley-Stott RP,Panamarova M,Zernicka-Goetz M,Surani MA,Silva JC

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Sat Dec 15 00:00:00 EST 2012

Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation.

Nature cell biology

Wray J,Kalkan T,Gomez-Lopez S,Eckardt D,Cook A,Kemler R,Smith A

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Immunofluorescence

Sun Jun 19 00:00:00 EDT 2011

A PiggyBac-based recessive screening method to identify pluripotency regulators.

PloS one

Guo G,Huang Y,Humphreys P,Wang X,Smith A

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Western Blot

Mon Apr 18 00:00:00 EDT 2011

A PiggyBac-based recessive screening method to identify pluripotency regulators.

PloS one

Guo G,Huang Y,Humphreys P,Wang X,Smith A

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Western Blot

Mon Apr 18 00:00:00 EDT 2011

Dioxin exposure disrupts the differentiation of mouse embryonic stem cells into cardiomyocytes.

Toxicological sciences : an official journal of the Society of Toxicology

Wang Y,Fan Y,Puga A

Published figure using Nanog monoclonal antibody (Product # 53-5761-80) in Western Blot

Sat May 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: early embryo specific expression NK family; Early embryo specific expression NK-type homeobox protein; ES cell-associated protein 4; FLJ12581; HGNC:20857; Homeobox protein NANOG; Homeobox transcription factor Nanog

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Gene Aliases: 2410002E02Rik; Ecat4; ENK; Nanog

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UniProt ID: (Mouse) Q80Z64

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Entrez Gene ID: (Mouse) 71950

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Function(s)

enhancer sequence-specific DNA binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding sequence-specific DNA binding transcription regulatory region DNA binding homeodomain transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter mesodermal cell fate commitment endodermal cell fate specification transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development cell proliferation positive regulation of cell proliferation embryo development response to organic substance negative regulation of cell fate commitment regulation of gene expression stem cell division stem cell population maintenance negative regulation of BMP signaling pathway response to retinoic acid somatic stem cell population maintenance negative regulation of endodermal cell fate specification regulation of cell differentiation negative regulation of cell differentiation positive regulation of transcription, DNA-templated positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter stem cell differentiation

Performance Guarantee

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Nanog Monoclonal Antibody (eBioMLC-51), Alexa Fluor™ 488, eBioscience™

Nanog Antibody (53-5761-80) in Flow

Product Details

53-5761-80

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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