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Invitrogen
Nephrin Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 20
Nephrin Antibody (PA5-20330) in IHC (P)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Nephrin Polyclonal Antibody (Product # PA5-20330) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary... View More
Product Details
PA5-20330
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 14 amino acid peptide from near the carboxy terminus of human Nephrin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11153994
Product Specific Information
Despite its predicted molecular weight, Nephrin often migrates at a lower molecular weight in SDS-PAGE. A suggested positive control is mouse kidney tissue lysate.
PA5-20330 can be used with blocking peptide PEP-0450.
Target Information
Primary steroid resistant nephrotic syndrome (SRNS) is characterized by childhood onset of proteinuria and progression to end stage renal disease. Kidney podocytes and their slit diaphragms form the final barrier to urinary protein loss. Congenital nephrotic syndrome (CNS) is caused by mutations in NPHS1 (nephrin) or NPHS2. Nephrin, a recently identified protein is a member of a group of podocyte proteins that constitute major component of the slit diaphragm especially in the foot process. Nephrin, a cell adhesion molecule, may play a crucial role in maintaining the glomerular filtration barrier. Recent studies have suggested that mutations in the gene for Nephrin reportedly lead to congenital nephrosis. Three novel podocyte proteins, Podocin, Nephrin and alpha Actinin 4 have been identified in congenital and experimental models of proteinuria. The role of Nephrin in anti apoptotic activity in podocyte slit diaphragm is believed to be associated with vascular endothelial derived growth factors VEGF signaling. Nephrin seems to play a role in the development or function of the kidney glomerular filtration barrier and it may anchor the podocyte slit diaphragm to the actin cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Nephrin; nephrin 1; nephrosis 1 homolog, nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHS1 nephrin; Renal glomerulus-specific cell adhesion receptor
Gene Aliases: CNF; nephrin; NephrinB; NPHN; NPHS1
UniProt ID: (Human) O60500, (Rat) Q9R044, (Mouse) Q9QZS7
Entrez Gene ID: (Human) 4868, (Rat) 64563, (Mouse) 54631
MAPK cascade
glomerular filtration
cell adhesion
JNK cascade
skeletal muscle tissue development
myoblast fusion
positive regulation of actin filament polymerization
glomerular basement membrane development
regulation of excretion
glomerular visceral epithelial cell development
multicellular organism development
muscle organ development
excretion
protein localization to synapse
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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