Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
Nestin Antibody (PA5-47378) in ICC/IF
Immunocytochemistry analysis of Nestin in rat cortical stem cells. Samples were incubated in Nestin polyclonal antibody (Product # PA5-47378) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green). Cells were also incubated with 5 µg/mL neuron-specific Mouse β‚III Tubulin Monoclonal (clone TuJ‚1) Antibody and stained for beta‚III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red). Tissue was counterstained with DAP... View More
Product Details
PA5-47378
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant rat Nestin Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609217
Product Specific Information
In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ21841; nestin
Entrez Gene ID: (Rat) 25491
G2/M transition of mitotic cell cycle
nervous system development
brain development
response to ionizing radiation
neuron differentiation
positive regulation of intermediate filament depolymerization
embryonic camera-type eye development
response to nutrient levels
negative regulation of protein binding
response to drug
negative regulation of catalytic activity
negative regulation of neuron apoptotic process
cell projection morphogenesis
stem cell proliferation
positive regulation of neural precursor cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support