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Invitrogen
Nestin Monoclonal Antibody (Rat-401 (4D4))
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
Nestin Antibody (MA1-81819) in ICC/IF
Immunofluorescence analysis of Nestin was performed using 70% confluent log phase PC-12 cells differentiated to neurons. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Nestin Monoclonal Antibody (Rat-401 (4D4)) (Product # MA1-81819) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary An... View More
Product Details
MA1-81819
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
Rat-401 (4D4)
Immunogen
Nestin purified from embryonic rat spinal cord.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2151134
Product Specific Information
This antibody does not react with human.
In Western blot, this antibody detects a ~200-220 kDa band in newborn rat or mouse cell extracts. For immunohistochemistry, we recommend perfusing tissues with 4% paraformaldehyde (pH 7.4) for light microscopy and with either 4% paraformaldehyde (pH 10.0) or 4% paraformaldehyde with 0.1% glutaraldehyde (pH 7.4) for EM. For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50mM sodium borate (pH 9.5). For Western blot, it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested.
Mouse anti Rat Nestin antibody, clone Rat-401 recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glian cells (Hockfield and McKay 1985).
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ21841; intermediate filament protein; Nestin
Gene Aliases: AA166324; C78523; ESTM46; Nes
UniProt ID: (Mouse) Q6P5H2
Entrez Gene ID: (Rat) 25491, (Mouse) 18008
G2/M transition of mitotic cell cycle
nervous system development
brain development
response to ionizing radiation
neuron differentiation
positive regulation of intermediate filament depolymerization
embryonic camera-type eye development
response to nutrient levels
negative regulation of protein binding
response to drug
negative regulation of catalytic activity
negative regulation of neuron apoptotic process
cell projection morphogenesis
stem cell proliferation
positive regulation of neural precursor cell proliferation
multicellular organism development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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