Invitrogen

NeuN Monoclonal Antibody (1B7)

NeuN Antibody in Immunocytochemistry (ICC/IF)

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NeuN Antibody (MA5-33103) in ICC/IF

Immunocytochemistry analysis of NeuN in cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with NeuN monoclonal antibody (Product # MA5-33103) using a dilution of 1:1,000 (red). The complete absence of astrocyte staining is shown using Anti-GFAP with a dilution of 1:1,000 (green) and nuclear staining was done with DAPI (blue).

NeuN Antibody in Immunohistochemistry (IHC)

NeuN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

MA5-33103

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunohistochemistry (IHC)

1:200-1:1,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:500

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1B7

Immunogen

Recombinant protein taken from the N-terminus of human FOX3 expressed in and purified from E. Coli

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 50% glycerol

Contains

5mM sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2802653

Target Information

Fox-3, also known as NEUN, HRNBP3, RBFOX3 (RNA binding protein, fox-1) or Fox-1 homolog C, is a 350 amino acid protein that contains one RRM (RNA recognition motif) domain. Localized to both the nucleus and cytoplasm, Fox-3 is suggested to regulate alternative splicing events. Fox-3 is encoded by a gene located on human chromosome 17, which is comprised over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and LiFraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting AAV vectors to the central nervous system by engineering capsid-receptor interactions that enable crossing of the blood-brain barrier.

PLoS biology

Huang Q,Chen AT,Chan KY,Sorensen H,Barry AJ,Azari B,Zheng Q,Beddow T,Zhao B,Tobey IG,Moncada-Reid C,Eid FE,Walkey CJ,Ljungberg MC,Lagor WR,Heaney JD,Chan YA,Deverman BE

MA5-33103 was used in Immunohistochemistry to demonstrate that AAV capsids can be directly targeted to specific proteins to generate potent gene delivery vectors with known mechanisms of action and predictable tropisms.

Sat Jul 01 00:00:00 EDT 2023

Icarisid II rescues cognitive dysfunction via activation of Wnt/β-catenin signaling pathway promoting hippocampal neurogenesis in APP/PS1 transgenic mice.

Phytotherapy research : PTR

Xiao HH,Chen JC,Li H,Li RH,Wang HB,Song HP,Li HY,Shan GS,Tian Y,Zhao YM,Tian JM,Yang JX

MA5-33103 was used in Immunohistochemistry-immunofluorescence to study whether icarisid II (ICS II), a natural product possessing powerful neuroprotection, could recover the neurogenesis dysfunction of APP/PS1 mice and the underlying mechanisms.

Sun May 01 00:00:00 EDT 2022

Icarisid II rescues cognitive dysfunction via activation of Wnt/β-catenin signaling pathway promoting hippocampal neurogenesis in APP/PS1 transgenic mice.

Phytotherapy research : PTR

Xiao HH,Chen JC,Li H,Li RH,Wang HB,Song HP,Li HY,Shan GS,Tian Y,Zhao YM,Tian JM,Yang JX

Sun May 01 00:00:00 EDT 2022

Dual-Specificity Protein Phosphatase 4 (DUSP4) Overexpression Improves Learning Behavior Selectively in Female 5xFAD Mice, and Reduces β-Amyloid Load in Males and Females.

Cells

Pan AL,Audrain M,Sakakibara E,Joshi R,Zhu X,Wang Q,Wang M,Beckmann ND,Schadt EE,Gandy S,Zhang B,Ehrlich ME,Salton SR

MA5-33103 was used in Immunohistochemistry-immunofluorescence to suggest that that gender-specific interventions may be indicated in the treatment of Alzheimers disease.

Thu Dec 01 00:00:00 EST 2022

Dual-Specificity Protein Phosphatase 4 (DUSP4) Overexpression Improves Learning Behavior Selectively in Female 5xFAD Mice, and Reduces β-Amyloid Load in Males and Females.

Cells

Pan AL,Audrain M,Sakakibara E,Joshi R,Zhu X,Wang Q,Wang M,Beckmann ND,Schadt EE,Gandy S,Zhang B,Ehrlich ME,Salton SR

MA5-33103 was used in Immunohistochemistry-immunofluorescence to suggest that that gender-specific interventions may be indicated in the treatment of Alzheimers disease.

Thu Dec 01 00:00:00 EST 2022

Overproduction of hydrogen sulfide, generated by cystathionine β-synthase, disrupts brain wave patterns and contributes to neurobehavioral dysfunction in a rat model of down syndrome.

Redox biology

Panagaki T,Lozano-Montes L,Janickova L,Zuhra K,Szabo MP,Majtan T,Rainer G,Maréchal D,Herault Y,Szabo C

MA5-33103 was used in Immunohistochemistry to invesitgate the functional role of the cystathionine-β-synthase (CBS)/hydrogen sulfide (H2S) pathway on the pathogenesis of brain wave pattern alterations and neurobehavioral dysfunction, using a novel rat model of Down syndrome (DS).

Sun May 01 00:00:00 EDT 2022

Use of high-content imaging to quantify transduction of AAV-PHP viruses in the brain following systemic delivery.

Brain communications

Smith EJ,Farshim PP,Flomen R,Jones ST,McAteer SJ,Deverman BE,Gradinaru V,Bates GP

MA5-33103 was used in Immunohistochemistry to allow the rapid comparison of transduction rates between brain regions producing comparable data to more time-consuming approaches.

Thu Mar 10 00:00:00 EST 2022

Use of high-content imaging to quantify transduction of AAV-PHP viruses in the brain following systemic delivery.

Brain communications

Smith EJ,Farshim PP,Flomen R,Jones ST,McAteer SJ,Deverman BE,Gradinaru V,Bates GP

MA5-33103 was used in Immunohistochemistry to allow the rapid comparison of transduction rates between brain regions producing comparable data to more time-consuming approaches.

Thu Mar 10 00:00:00 EST 2022

UNC-45A Is Highly Expressed in the Proliferative Cells of the Mouse Genital Tract and in the Microtubule-Rich Areas of the Mouse Nervous System.

Cells

Clemente V,Hoshino A,Meints J,Shetty M,Starr T,Lee M,Bazzaro M

MA5-33103 was used in Immunohistochemistry to suggest that UNC-45A may have a role in ovarian cancer initiation and development as well as in neurodegeneration.

Sat Jun 26 00:00:00 EDT 2021

UNC-45A Is Highly Expressed in the Proliferative Cells of the Mouse Genital Tract and in the Microtubule-Rich Areas of the Mouse Nervous System.

Cells

Clemente V,Hoshino A,Meints J,Shetty M,Starr T,Lee M,Bazzaro M

MA5-33103 was used in Immunohistochemistry to suggest that UNC-45A may have a role in ovarian cancer initiation and development as well as in neurodegeneration.

Sat Jun 26 00:00:00 EDT 2021

Active constituent of Polygala tenuifolia attenuates cognitive deficits by rescuing hippocampal neurogenesis in APP/PS1 transgenic mice.

BMC complementary medicine and therapies

Wang XF,Xiao HH,Wu YT,Kong L,Chen JC,Yang JX,Hu XL

Published figure using NeuN monoclonal antibody (Product # MA5-33103) in Immunocytochemistry

Mon Oct 25 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: FLJ56884; FLJ58356; fox 1 homolog (C. elegans) 3; Fox-1 homolog C; Fox-3; fox1 homolog C; FOX3NeuN; hexaribonucleotide binding protein 3; hexaribonucleotide binding protein 3 (HRNBP3); Hexaribonucleotide-binding protein 3; NeuN antigen; neuronal nuclear antigen A60; neuronal nuclei; Neuronal nuclei antigen; RFOX3; RNA binding protein; RNA binding protein fox-1 homolog 3; RNA binding protein, fox-1 homolog (C. elegans) 3; RNA binding protein, fox-1 homolog 3

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Gene Aliases: D11Bwg0517e; FOX-3; FOX3; HRNBP3; NEUN; Neuna60; RBFOX3; RGD1560070

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UniProt ID: (Bovine) Q0VD23, (Human) A6NFN3, (Mouse) Q8BIF2

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Entrez Gene ID: (Bovine) 511773, (Human) 146713, (Rat) 287847, (Mouse) 52897

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Function(s)

nucleotide binding DNA binding mRNA binding RNA binding nucleic acid binding protein binding RNA metabolism protein RNA processing factor RNA splicing factor nucleic acid metabolism protein

Process(es)

mRNA processing RNA splicing regulation of alternative mRNA splicing, via spliceosome regulation of RNA splicing

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NeuN Monoclonal Antibody (1B7)

NeuN Antibody (MA5-33103) in ICC/IF

Product Details

MA5-33103

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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