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NeuN Antibody (MA5-50648) in IHC (P)
Immunohistochemistry of NeuN in paraffin-embedded mouse hippocampus tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with NeuN recombinant monoclonal antibody (Product # MA5-50648) with a dilution of 1:1,000 for 1 hour at ro... View More
Product Details
MA5-50648
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PD01-45
Immunogen
Synthetic peptide within human NeuN aa 20-60/312.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3093737
Product Specific Information
Positive control: SH-SY5Y cell lysate, SHG44 cell lysate, Human brain tissue, mouse hippocampus tissue, mouse brain tissue, mouse cerebellum tissue, rat hippocampus tissue, rat brain tissue, rat cerebellum tissue, mouse cerebellum tissue lysates, mouse cerebral cortex tissue.
Predicted band size: 34 kDa
Subcellular Location: Cytoplasm, Nucleus.
Target Information
Fox-3, also known as NEUN, HRNBP3, RBFOX3 (RNA binding protein, fox-1) or Fox-1 homolog C, is a 350 amino acid protein that contains one RRM (RNA recognition motif) domain. Localized to both the nucleus and cytoplasm, Fox-3 is suggested to regulate alternative splicing events. Fox-3 is encoded by a gene located on human chromosome 17, which is comprised over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and LiFraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ56884; FLJ58356; fox 1 homolog (C. elegans) 3; Fox-1 homolog C; Fox-3; fox1 homolog C; FOX3NeuN; hexaribonucleotide binding protein 3; hexaribonucleotide binding protein 3 (HRNBP3); Hexaribonucleotide-binding protein 3; NeuN antigen; neuronal nuclear antigen A60; neuronal nuclei; Neuronal nuclei antigen; RFOX3; RNA binding protein; RNA binding protein fox-1 homolog 3; RNA binding protein, fox-1 homolog (C. elegans) 3; RNA binding protein, fox-1 homolog 3
Gene Aliases: D11Bwg0517e; FOX-3; FOX3; HRNBP3; NEUN; Neuna60; RBFOX3; RGD1560070
UniProt ID: (Human) A6NFN3, (Mouse) Q8BIF2
Entrez Gene ID: (Human) 146713, (Rat) 287847, (Mouse) 52897
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