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Invitrogen
Neural/Glial Antigen 2 (NG2) Monoclonal Antibody (9.2.27), Alexa Fluor™ 488, eBioscience™
FIGURE: 1 / 7
Neural/Glial Antigen 2 (NG2) Antibody (53-6504-82) in ICC/IF
Immunocytochemistry on fixed A375 cells using 20 µg/mL of Mouse IgG2a K Isotype control Alexa Fluor® 488 (left) or 20 µg/mL Anti-Human Neural/Glial Antigen 2 (NG2) Alexa Fluor® 488 (right). Nuclei are counterstained with DAPI.
Product Details
53-6504-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
9.2.27
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10853964
Product Specific Information
Description: This 9.2.27 monoclonal antibody reacts with human neural/glial antigen 2, which is also known as melanoma chondroitin sulfate proteoglycan (MCSP). This antigen is composed of a 250 kDa N-linked glycoprotein and a >450 kDa proteoglycan. NG2 is present on the surface of >90% of malignant melanomas in addition to some non-melanomic tumors. Moreover, NG2 is expressed on glioma cells, as well as on developing and adult oligodendrocyte precursor cells. Studies have demonstrated the involvement of this protein in tumor cell proliferation, adhesion, migration, and invasion. NG2 also functions as a coreceptor for alpha4beta1 integrin. Finally, expression of NG2 can be used as a prognostic marker for disorders such as acral letiginous melanoma and infantile acute myeloid leukemia.
The 9.2.27 antibody has been reported to suppress melanoma tumor growth.
Applications Reported: This 9.2.27 has been reported for use in flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded tissue (IHC-P), and immunocytochemical staining of fixed cells (ICC).
Applications Tested: This 9.2.27 antibody has been tested by immunocytochemistry on methanol-fixed A375 cells at less than or equal to 20 µg/mL and by flow cytometric analysis on A375 cells at less than or equal to 0.125 µg/test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10e5 to 10e8 cells/test. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4 (melanoma-associated); Chondroitin sulfate proteoglycan NG2; HMW MAA; MEL CSPG; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan
Gene Aliases: CSPG4; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2
UniProt ID: (Human) Q6UVK1
Entrez Gene ID: (Human) 1464
activation of MAPK activity
angiogenesis
transmembrane receptor protein tyrosine kinase signaling pathway
cell proliferation
glial cell migration
cell migration
glycosaminoglycan metabolic process
chondroitin sulfate biosynthetic process
chondroitin sulfate catabolic process
dermatan sulfate biosynthetic process
intracellular signal transduction
tissue remodeling
positive regulation of peptidyl-tyrosine phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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