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Invitrogen
Neural/Glial Antigen 2 (NG2) Recombinant Rabbit Monoclonal Antibody (JM10-13)
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Neural/Glial Antigen 2 (NG2) Antibody (MA5-32579) in IHC (P)
Immunohistochemistry analysis of NG2 in paraffin-embedded human malignant melanoma tissue. Samples were incubated in NG2 monoclonal antibody (Product # MA5-32579) using a dilution of 1:400. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then ... View More
Product Details
MA5-32579
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JM10-13
Immunogen
Synthetic peptide within Human NG2 aa 2271-2314
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809856
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4 (melanoma-associated); Chondroitin sulfate proteoglycan NG2; HMW MAA; MEL CSPG; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan
Gene Aliases: CSPG4; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2
UniProt ID: (Human) Q6UVK1
Entrez Gene ID: (Human) 1464
activation of MAPK activity
angiogenesis
transmembrane receptor protein tyrosine kinase signaling pathway
cell proliferation
glial cell migration
cell migration
glycosaminoglycan metabolic process
chondroitin sulfate biosynthetic process
chondroitin sulfate catabolic process
dermatan sulfate biosynthetic process
intracellular signal transduction
tissue remodeling
positive regulation of peptidyl-tyrosine phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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