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Invitrogen
NeuroD1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
NeuroD1 Antibody (PA5-47381) in ICC/IF
Immunofluorescence analysis of NeuroD1 was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NeuroD1 Goat Polyclonal Antibody (Product # PA5-47381) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11078) at a dilu... View More
Product Details
PA5-47381
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human NeuroD1 Ser154-Asp356
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2607915
Product Specific Information
Approximately 5% cross-reactivity with recombinant human NeuroD2 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
NeuroD1 is a transcriptional activator that acts as a differentiation factor during neurogenesis. It has been demonstrated to bind to the insulin gene E-box. Efficient DNA binding requires dimerization with another basic helix-loop-helix (bHLH) protein. Defects in NEUROD1 are a cause of maturity onset diabetes of the young type VI (MODY6). MODY6 is a form of non-insulin-dependent diabetes mellitus characterized by an autosomal dominant mode of inheritance, onset during young adulthood and a primary defect in insulin secretion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: basic helix-loop-helix transcription factor; beta-cell E-box transactivator 2; Beta-cell E-box transcriptional activator 2; Beta2; BHF1; bHLHa3; Class A basic helix-loop-helix protein 3; neuro d1; NeuroD; NeuroD1; Neurogenic differentiation factor 1; neurogenic helix-loop-helix protein NEUROD
Gene Aliases: BETA2; BHF-1; BHLHA3; MODY6; NEUROD; NEUROD1
UniProt ID: (Human) Q13562, (Mouse) Q60867
Entrez Gene ID: (Human) 4760, (Mouse) 18012
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
RNA polymerase II transcription coactivator activity
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
transcription factor binding
sequence-specific DNA binding
protein heterodimerization activity
E-box binding
DNA binding
double-stranded DNA binding
protein dimerization activity
basic helix-loop-helix transcription factor
pancreatic A cell fate commitment
pancreatic PP cell fate commitment
transcription from RNA polymerase II promoter
nucleocytoplasmic transport
nitric oxide mediated signal transduction
response to glucose
anterior/posterior pattern specification
dentate gyrus development
cerebellum development
neurogenesis
signal transduction involved in regulation of gene expression
insulin secretion
endocrine pancreas development
amacrine cell differentiation
enteroendocrine cell differentiation
response to drug
glucose homeostasis
positive regulation of apoptotic process
positive regulation of cell differentiation
positive regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JAK-STAT cascade
embryonic organ morphogenesis
neuron development
inner ear development
regulation of insulin secretion
positive regulation of sequence-specific DNA binding transcription factor activity
regulation of intestinal epithelial structure maintenance
regulation of cell cycle arrest
cellular response to glucose stimulus
negative regulation of type B pancreatic cell apoptotic process
positive regulation of transcription regulatory region DNA binding
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
nervous system development
cell differentiation
hindbrain development
camera-type eye development
cell fate commitment
regulation of neuron differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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