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Neurogenin 2 Antibody (PA5-80851) in ICC/IF
Immunofluorescence staining of Neurogenin 2 in HESS9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Neurogenin 2 Polyclonal Antibody (Product # PA5-80851, 1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
Product Details
PA5-80851
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the center region of the Human NEUROG2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788120
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human NEUROG2.
Target Information
Neurogenin-2 is a member of the neurogenin subfamily of basic helix-loop-helix (bHLH) transcription factor genes that play an important role in neurogenesis from migratory neural crest cells. In the course of mouse neurogenesis, Ngn2 and Ngn1 are expressed in distinct progenitor populations residing in the central and peripheral nervous systems. Mutation analyses demonstrate that Ngn1 is required for the determination of neuronal precursors for proximal cranial sensory ganglia and that Ngn2 is necessary for the determination of precursors for epibranchial placode-derived sensory neurons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHa8; Class A basic helix-loop-helix protein 8; Neurogenin-2; neurogenin2; NGN-2; Protein atonal homolog 4
Gene Aliases: ATOH4; BHLHA8; Math4A; NEUROG2; ngn-2; NGN2
UniProt ID: (Human) Q9H2A3
Entrez Gene ID: (Human) 63973
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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