Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Alomone Labs, LTD
Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Polyclonal Antibody
FIGURE: 1 / 4
Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody (ANC-002-200UL) in ICC/IF
Expression of nAChR alpha 2 in rat PC12 pheochromocytoma cells - Cell surface detection of nAChR alpha 2 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of live cells with Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:50), followed by goat Anti-rabbit-AlexaFluor-594 (red). B. Live image of the cells. C. Merge of the two images.
Product Details
ANC-002-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)DLEQMERTVDLKD, corresponding to amino acid residues 189-201 of rat nAChR alpha 2, Extracellular, N-terminus
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
After binding acetylcholine, the ACHR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: acetylcholine receptor; acetylcholine receptor, nicotinic, alpha 2 (neuronal); cholinergic receptor; cholinergic receptor, nicotinic alpha 2; cholinergic receptor, nicotinic, alpha 2 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal); Neuronal acetylcholine receptor subunit alpha-2; nicotinic acetylcholine receptor
Gene Aliases: ACHR; Acra-2; Acra2; BC011490; CHRNA2
UniProt ID: (Human) Q15822, (Rat) P12389, (Mouse) Q91X60
Entrez Gene ID: (Human) 1135, (Rat) 170945, (Mouse) 110902
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support