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Invitrogen
Nod2 Monoclonal Antibody (26mNOD2), eBioscience™
Product Details
14-5858-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
26mNOD2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1107022
Product Specific Information
Description: The monoclonal antibody 26mNOD2 reacts with mouse NOD2. NOD2, also referred to as caspase recruitment domain-containing protein 15 (CARD 15), is a cytosolic protein of approximately 115kDa. NOD2 contains a central NOD (nucleotide oligomerization domain) that is flanked by a C-terminal leucine-rich repeat (LRR) domain and an N-terminal CARD domain. The LRR confers recognition of microbial components such as muramyl dipeptide and the CARD domain confers the ability to interact with RIP2 via homotypic interaction with the RIP2 CARD. This interaction leads to downstream activation of NF-kappaB.
The antibody 26mNOD2 has been shown to recognize protein from transgenic and transfected cells. Refer to references for expression in endogenous cells.
Applications Reported: This 26mNOD2 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This 26mNOD2 antibody has been tested by immunoblotting of lysates of 293T cells transfected with mouse NOD2. This antibody has been validated on transfected cell lines and cells from NOD2-transgenic mice.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
NOD2 is a member of the apoptosis regulating protein family that includes caspase recruitment-domains, as well as Apaf-1 and NOD1. It contains two N-terminal CARDs, a nucleotide binding domain (NBD), and multiple C-terminal leucine-rich repeats (LRRs). NOD2 is expressed in monocytes (whereas NOD1 is expressed in multiple tissues). NOD2 plays a role in regulating NF-kappaB, acts as an intracellular receptor for bacterial lipopolysaccharides, and contributes to inflammatory bowel disease (IBD) and Crohn's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: caspase recruitment domain family, member 15; caspase recruitment domain-containing protein 15; NOD 2; NOD-2; NOD2 protein; nucleotide-binding oligomerization domain-containing protein 2
Gene Aliases: ACUG; BLAU; Card15; CD; F830032C23Rik; IBD1; Nlrc2
Entrez Gene ID: (Mouse) 257632
innate immune response in mucosa
activation of immune response
cytokine production involved in immune response
cytokine secretion involved in immune response
immune system process
immunoglobulin production involved in immunoglobulin mediated immune response
positive regulation of dendritic cell antigen processing and presentation
negative regulation of T cell mediated immunity
positive regulation of dendritic cell cytokine production
positive regulation of type 2 immune response
negative regulation of inflammatory response to antigenic stimulus
positive regulation of humoral immune response mediated by circulating immunoglobulin
defense response
inflammatory response
positive regulation of antibacterial peptide biosynthetic process
positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria
signal transduction
positive regulation of cell proliferation
detection of biotic stimulus
detection of bacterium
maintenance of gastrointestinal epithelium
positive regulation of protein ubiquitination
negative regulation of NF-kappaB transcription factor activity
response to peptidoglycan
response to muramyl dipeptide
response to lipopolysaccharide
detection of muramyl dipeptide
negative regulation of interferon-gamma production
negative regulation of interleukin-12 production
negative regulation of interleukin-18 production
negative regulation of interleukin-2 production
negative regulation of tumor necrosis factor production
positive regulation of interleukin-1 beta production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-17 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
positive regulation of stress-activated MAPK cascade
negative regulation of toll-like receptor 2 signaling pathway
activation of MAPK activity involved in innate immune response
intracellular signal transduction
defense response to bacterium
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to exogenous dsRNA
positive regulation of MAPK cascade
positive regulation of phosphatidylinositol 3-kinase activity
negative regulation of growth of symbiont in host
innate immune response
positive regulation of innate immune response
positive regulation of Notch signaling pathway
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
positive regulation of epithelial cell proliferation
positive regulation of interleukin-1 beta secretion
regulation of inflammatory response
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of phagocytosis
defense response to Gram-positive bacterium
positive regulation of B cell activation
positive regulation of NF-kappaB transcription factor activity
protein oligomerization
positive regulation of oxidoreductase activity
positive regulation of nitric-oxide synthase biosynthetic process
positive regulation of prostaglandin-endoperoxide synthase activity
positive regulation of ERK1 and ERK2 cascade
nucleotide-binding oligomerization domain containing 2 signaling pathway
cellular response to peptidoglycan
cellular response to muramyl dipeptide
macrophage inflammatory protein-1 alpha production
regulation of neutrophil chemotaxis
positive regulation of cytokine production involved in inflammatory response
negative regulation of macrophage apoptotic process
positive regulation of prostaglandin-E synthase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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