Invitrogen

Nrf2 Polyclonal Antibody

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5 Published Figures

8 References

View all (42) Nrf2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Nrf2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

Nrf2 Antibody (PA5-88084) in ICC/IF

Immunofluorescence analysis of Nrf2 in HeLa cells. Samples were incubated with Nrf2 Polyclonal antibody (Product # PA5-88084) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Nrf2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Nrf2 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Nrf2 Polyclonal Antibody (Product # PA5-88084), shows the upregulation of Nrf2 expression upon treatment with MG-132 and TBHQ in U-2 OS, MDA-MB-231, Hep G2 and HEK-293 cell lines. {TM}

Product Details

PA5-88084

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 366-605 of human NRF2 (NP_0061552)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.92 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804638

Product Specific Information

Positive Samples: A-549, HepG2, HeLa, Rat liver; Cellular Location: Cytoplasm, Nucleus, cytosol

Immunogen sequence: YGDTLLGLSD SEVEELDSAP GSVKQNGPKT PVHSSGDMVQ PLSPSQGQST HVHDAQCENT PEKELPVSPG HRKTPFTKDK HSSRLEAHLT RDELRAKALH IPFPVEKIIN LPVVDFNEMM SKEQFNEAQL ALIRDIRRRG KNKVAAQNCR KRKLENIVEL EQDLDHLKDE KEKLLKEKGE NDKSLHLLKK QLSTLYLEVF SMLRDEDGKP YSPSEYSLQQ TRDGNVFLVP KSKKPDVKKN

Target Information

Nfr1 (also designated TCF11), Nrf2 and Nfr3 are bZIP transcription factors that heterodimer with Maf proteins to bind MARE (Maf recognition elements) sequences. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The cardioprotective effects of secoisolariciresinol diglucoside (flaxseed lignan) against cafeteria diet-induced cardiac fibrosis and vascular injury in rats: an insight into apelin/AMPK/FOXO3a signaling pathways.

Frontiers in pharmacology

Abdelwahab AH,Negm AM,Mahmoud ES,Salama RM,Schaalan MF,El-Sheikh AAK,Ramadan BK

Published figure using Nrf2 polyclonal antibody (Product # PA5-88084) in Western Blot

Fri Jul 28 00:00:00 EDT 2023

Gut microbiota-derived tryptophan metabolites alleviate liver injury via AhR/Nrf2 activation in pyrrolizidine alkaloids-induced sinusoidal obstruction syndrome.

Cell & bioscience

Shang H,Huang C,Xiao Z,Yang P,Zhang S,Hou X,Zhang L

PA5-88084 was used in Western Blotting to investigate the role of gut microbiota in hepatic sinusoidal obstruction syndrome (HSOS), highlighting the impact of microbial tryptophan metabolism and the AhR/Nrf2 signaling pathway in MCT-induced liver injury.

Sat Jul 08 00:00:00 EDT 2023

Flavonoid-Conjugated Gadolinium Complexes as Anti-Inflammatory Theranostic Agents.

Antioxidants (Basel, Switzerland)

Yang BW,Yang S,Kim S,Baek AR,Sung B,Kim YH,Lee JT,Lee SY,Kim HK,Choi G,Park JA,Nam SW,Lee GH,Chang Y

Published figure using Nrf2 polyclonal antibody (Product # PA5-88084) in Western Blot

Thu Dec 15 00:00:00 EST 2022

Galangin attenuates cadmium-evoked nephrotoxicity: Targeting nucleotide-binding domain-like receptor pyrin domain containing 3 inflammasome, nuclear factor erythroid 2-related factor 2, and nuclear factor kappa B signaling.

Journal of biochemical and molecular toxicology

Salama SA,Abd-Allah GM,Gad HS,Kabel AM

PA5-88084 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to highlight the ability of galangin to attenuate cadmium-evoked nephrotoxicity and support its therapeutic implementation although clinical investigations are warranted.

Fri Jul 01 00:00:00 EDT 2022

Journal of biochemical and molecular toxicology

Salama SA,Abd-Allah GM,Gad HS,Kabel AM

Fri Jul 01 00:00:00 EDT 2022

Short-term exposure to JUUL electronic cigarettes can worsen ischemic stroke outcome.

Fluids and barriers of the CNS

Sifat AE,Archie SR,Nozohouri S,Villalba H,Zhang Y,Sharma S,Ghanwatkar Y,Vaidya B,Mara D,Cucullo L,Abbruscato TJ

PA5-88084 was used in Western Blotting to suggest that JUUL exposure could negatively impact the cerebrovascular system, although to a lesser extent than TS exposure.

Fri Sep 09 00:00:00 EDT 2022

Short-term exposure to JUUL electronic cigarettes can worsen ischemic stroke outcome.

Fluids and barriers of the CNS

Sifat AE,Archie SR,Nozohouri S,Villalba H,Zhang Y,Sharma S,Ghanwatkar Y,Vaidya B,Mara D,Cucullo L,Abbruscato TJ

PA5-88084 was used in Western Blotting to suggest that JUUL exposure could negatively impact the cerebrovascular system, although to a lesser extent than TS exposure.

Fri Sep 09 00:00:00 EDT 2022

circ_0072464 Shuttled by Bone Mesenchymal Stem Cell-Secreted Extracellular Vesicles Inhibits Nucleus Pulposus Cell Ferroptosis to Relieve Intervertebral Disc Degeneration.

Oxidative medicine and cellular longevity

Yu X,Xu H,Liu Q,Wang Y,Wang S,Lu R,Jiang Y,Kang H,Hu W

PA5-88084 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that BMSC-EV-loaded circ_0072464 inhibited NPC ferroptosis to relieve IDD via upregulation of miR-431-mediated NRF2, therefore providing a potential therapeutic target against IDD.

Tue Jul 12 00:00:00 EDT 2022

Impact of chronic smoking on traumatic brain microvascular injury: An in vitro study.

Journal of cellular and molecular medicine

Sivandzade F,Alqahtani F,Cucullo L

PA5-88084 was used in Western Blot to show that chronic TS exposure exacerbates traumatic endothelial injury and abrogates the protective antioxidative cell responses.

Sun Aug 01 00:00:00 EDT 2021

Lysosomal nitric oxide determines transition from autophagy to ferroptosis after exposure to plasma-activated Ringer's lactate.

Redox biology

Jiang L,Zheng H,Lyu Q,Hayashi S,Sato K,Sekido Y,Nakamura K,Tanaka H,Ishikawa K,Kajiyama H,Mizuno M,Hori M,Toyokuni S

PA5-88084 was used in Immunocytochemistry to show that PAL induces selective ferroptosis of malignant mesothelioma (MM) cells, where non-targeted metabolome screening identified upregulated citrulline-nitric oxide (.NO) cycle as a PAL target.

Thu Jul 01 00:00:00 EDT 2021

Lysosomal nitric oxide determines transition from autophagy to ferroptosis after exposure to plasma-activated Ringer's lactate.

Redox biology

Jiang L,Zheng H,Lyu Q,Hayashi S,Sato K,Sekido Y,Nakamura K,Tanaka H,Ishikawa K,Kajiyama H,Mizuno M,Hori M,Toyokuni S

Thu Jul 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: E4TF1-60; E4TF1A; NF-E2-related factor 2; NFE2-related factor 2; NFT2; nrf2; NRF2A; nuclear factor erythroid 2-like 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid 2-like 2; Nuclear factor, erythroid derived 2, like 2

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Gene Aliases: AI194320; HEBP1; NFE2L2; NRF2

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UniProt ID: (Human) Q16236, (Mouse) Q60795, (Rat) O54968

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Entrez Gene ID: (Human) 4780, (Mouse) 18024, (Rat) 83619

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Function(s)

transcription regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcription cofactor binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein domain specific binding transcription regulatory region DNA binding sequence-specific DNA binding protein heterodimerization activity basic leucine zipper transcription factor

Process(es)

Performance Guarantee

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Nrf2 Polyclonal Antibody

Nrf2 Antibody (PA5-88084) in ICC/IF

Product Details

PA5-88084

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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