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Invitrogen
O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (HGAC85)
FIGURE: 1 / 12
O-linked N-acetylglucosamine (O-GlcNAc) Antibody (MA1-076) in ICC/IF
Immunofluorescence analysis of OGT was performed using 70% confluent log phase Panc-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with OGT (HGAC85) Mouse Monoclonal Antibody (Product # MA1-076) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at... View More
Product Details
MA1-076
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical,
Fruit fly,
Hamster,
Human,
Mouse,
Nematode,
Rabbit,
Rat,
Sheep
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Clone
HGAC85
Immunogen
Heat killed, pepsin treated group A streptococci.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites, PBS
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_326365
Product Specific Information
MA1-076 recognizes beta-1,3 linked O-linked N-acetylglucosamine (O-GlcNAc) residues of streptococcal group A carbohydrate as well as O-GlcNAc glycosylated proteins.
MA1-076 has been successfully used in Western blot, immunofluorescence, immunoprecipitation and ELISA procedures. By Western blot, this antibody detects several proteins representing immunoprecipitated O-GlcNAc glycoproteins. Immunofluorescence staining of O-GlcNAc in cells results in labeling of the nuclear envelope and pores, nucleolus, and cytoplasm. This staining pattern is consistent with other methods of detecting O-GlcNAc moieties.
The MA1-076 immunogen is heat killed, pepsin treated group A streptococci.
DO NOT USE WITH DILUENTS CONTAINING GLYCOSYLATED PROTEINS.
Target Information
O-linked N-acetylglucosamine (O-GlcNAc) is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine or threonine residues. Unlike other protein glycosylations, O-GlcNAc modifications occur within the nucleus and cytoplasm. They are found on many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins. O-GlcNAc glycosylations are thought to obscure phosphorylation sites, counteracting phosphorylation-dependent signaling pathways and protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GlcNAc
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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