Invitrogen

OCT3/4 Monoclonal Antibody (EM92), eFluor™ 660, eBioscience™

OCT3/4 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 7

OCT3/4 Antibody (50-5841-82) in Flow

Intracellular staining of F9 cells with 0.5 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (blue histogram) or Anti-Human/Mouse OCT3/4 eFluor® 660 (purple histogram) using the Foxp3 Staining Buffer Set (Product # 00-5523-00) and protocol. Total cells were used for analysis.

OCT3/4 Antibody in Immunocytochemistry (ICC/IF)

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Product Details

50-5841-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

EM92

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11218882

Product Specific Information

Description: The EM92 monoclonal antibody reacts with mouse and human Oct3/4, encoded by the Pou5F1 gene. Oct3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem (ES) and induced pluripotent stem (iPS) cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass (ICM), reduction of Oct3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor (LIF).

Applications Reported: This EM92 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This EM92 antibody has been tested by intracellular staining and flow cytometric analysis of the F9 cell line using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CBP-mediated Wnt3a/β-catenin signaling promotes cervical oncogenesis initiated by Piwil2.

Neoplasia (New York, N.Y.)

Feng D,Yan K,Liang H,Liang J,Wang W,Yu H,Zhou Y,Zhao W,Dong Z,Ling B

50-5841 was used in Flow cytometry/Cell sorting to determine that canonical Wnt signaling was aberrantly activated in HaCaT cells transfected with lentivirus expressing Piwil2 and in cervical lesion specimens of low-grade squamous intraepithelial lesion, high-grade squamous intraepithelial lesion, and invasive carcinoma.

Fri Jan 01 00:00:00 EST 2021

CBP-mediated Wnt3a/β-catenin signaling promotes cervical oncogenesis initiated by Piwil2.

Neoplasia (New York, N.Y.)

Feng D,Yan K,Liang H,Liang J,Wang W,Yu H,Zhou Y,Zhao W,Dong Z,Ling B

Fri Jan 01 00:00:00 EST 2021

Comparative analysis of CI- and CIV-containing respiratory supercomplexes at single-cell resolution.

Cell reports methods

Bertan F,Wischhof L,Scifo E,Guranda M,Jackson J,Marsal-Cots A,Piazzesi A,Stork M,Peitz M,Prehn JHM,Ehninger D,Nicotera P,Bano D

Published figure using OCT3/4 monoclonal antibody (Product # 50-5841-82) in Immunocytochemistry

Mon May 24 00:00:00 EDT 2021

Human induced pluripotent stem cells ameliorate hyperoxia-induced lung injury in a mouse model.

American journal of translational research

Mitchell A,Wanczyk H,Jensen T,Finck C

50-5841 was used in Flow cytometry/Cell sorting to determine if iPSCs and diPSCs could repair hyperoxia-induced lung damage in a mouse model.

Mon Sep 28 00:00:00 EDT 2020

Pressure-Driven Mitochondrial Transfer Pipeline Generates Mammalian Cells of Desired Genetic Combinations and Fates.

Cell reports

Patananan AN,Sercel AJ,Wu TH,Ahsan FM,Torres A,Kennedy SAL,Vandiver A,Collier AJ,Mehrabi A,Van Lew J,Zakin L,Rodriguez N,Sixto M,Tadros W,Lazar A,Sieling PA,Nguyen TL,Dawson ER,Braas D,Golovato J,Cisneros L,Vaske C,Plath K,Rabizadeh S,Niazi KR,Chiou PY,Teitell MA

Published figure using OCT3/4 monoclonal antibody (Product # 50-5841-82) in Immunocytochemistry

Tue Dec 29 00:00:00 EST 2020

TAF5L and TAF6L Maintain Self-Renewal of Embryonic Stem Cells via the MYC Regulatory Network.

Molecular cell

Seruggia D,Oti M,Tripathi P,Canver MC,LeBlanc L,Di Giammartino DC,Bullen MJ,Nefzger CM,Sun YBY,Farouni R,Polo JM,Pinello L,Apostolou E,Kim J,Orkin SH,Das PP

Published figure using OCT3/4 monoclonal antibody (Product # 50-5841-82) in Flow Cytometry

Thu Jun 20 00:00:00 EDT 2019

FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation.

Cell reports

Lee K,Cho H,Rickert RW,Li QV,Pulecio J,Leslie CS,Huangfu D

50-5841-82 was used in Flow cytometry/Cell sorting to investigate the pioneer factor requirement for FOXA2 using human pluripotent stem cells.

Tue Jul 09 00:00:00 EDT 2019

FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation.

Cell reports

Lee K,Cho H,Rickert RW,Li QV,Pulecio J,Leslie CS,Huangfu D

50-5841-82 was used in Flow cytometry/Cell sorting to investigate the pioneer factor requirement for FOXA2 using human pluripotent stem cells.

Tue Jul 09 00:00:00 EDT 2019

Glutamine independence is a selectable feature of pluripotent stem cells.

Nature metabolism

Vardhana SA,Arnold PK,Rosen BP,Chen Y,Carey BW,Huangfu D,Carmona Fontaine C,Thompson CB,Finley LWS

50-5841-82 was used in Flow Cytometry to show that reduced dependence on exogenous glutamine is a generalizable feature of pluripotent stem cells.

Mon Jul 01 00:00:00 EDT 2019

Analyses of a Mutant Foxp3 Allele Reveal BATF as a Critical Transcription Factor in the Differentiation and Accumulation of Tissue Regulatory T Cells.

Immunity

Hayatsu N,Miyao T,Tachibana M,Murakami R,Kimura A,Kato T,Kawakami E,Endo TA,Setoguchi R,Watarai H,Nishikawa T,Yasuda T,Yoshida H,Hori S

50-5841 was used in Flow cytometry/Cell sorting to identify BATF as a regulator of tissue regulatory T cells and suggest that sequence-specific perturbations of Foxp3-DNA interactions can influence specific facets of Treg physiology and the immunopathologies.

Tue Aug 15 00:00:00 EDT 2017

Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells.

Nucleic acids research

Byrne SM,Ortiz L,Mali P,Aach J,Church GM

50584182 was used in flow cytometry to describe methods to efficiently 'knock-in' multi-kilobase genes in human induced pluripotent stem cells

Wed Feb 18 00:00:00 EST 2015

Developmental-like bone regeneration by human embryonic stem cell-derived mesenchymal cells.

Tissue engineering. Part A

Kuhn LT,Liu Y,Boyd NL,Dennis JE,Jiang X,Xin X,Charles LF,Wang L,Aguila HL,Rowe DW,Lichtler AC,Goldberg AJ

50-5841 was used in Flow cytometry/Cell sorting to evaluate the in vivo osteogenesis potential of mesenchymal-like cells derived from human embryonic stem cells.

Wed Jan 01 00:00:00 EST 2014

Highly upregulated Lhx2 in the Foxn1-/- nude mouse phenotype reflects a dysregulated and expanded epidermal stem cell niche.

PloS one

Bohr S,Patel SJ,Vasko R,Shen K,Huang G,Yarmush ML,Berthiaume F

Published figure using OCT3/4 monoclonal antibody (Product # 50-5841-82) in Flow Cytometry

Mon Dec 23 00:00:00 EST 2013

Bioinformatics

Protein Aliases: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2

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Gene Aliases: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1

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UniProt ID: (Human) Q01860, (Mouse) P20263

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Entrez Gene ID: (Human) 5460, (Mouse) 18999

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Function(s)

transcription regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding ubiquitin protein ligase binding miRNA binding sequence-specific DNA binding poly(A) RNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription coactivator activity chromatin binding transcription corepressor activity transcription factor binding chromatin DNA binding transcription regulatory region DNA binding protein heterodimerization activity POU domain binding HMG box domain binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter mesodermal cell fate commitment endodermal cell fate commitment ectodermal cell fate commitment endodermal cell fate specification trophectodermal cell differentiation blastocyst growth BMP signaling pathway involved in heart induction transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development response to organic substance regulation of gene expression stem cell population maintenance germ-line stem cell population maintenance response to retinoic acid somatic stem cell population maintenance positive regulation of catenin import into nucleus mRNA transcription from RNA polymerase II promoter cell fate commitment negative regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of calcium ion-dependent exocytosis stem cell differentiation positive regulation of protein kinase B signaling negative regulation of protein kinase B signaling positive regulation of SMAD protein import into nucleus cell fate commitment involved in formation of primary germ layer cardiac cell fate determination negative regulation of gene silencing by miRNA regulation of heart induction by regulation of canonical Wnt signaling pathway regulation of methylation-dependent chromatin silencing blastocyst development response to wounding anatomical structure morphogenesis regulation of asymmetric cell division

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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OCT3/4 Monoclonal Antibody (EM92), eFluor™ 660, eBioscience™

OCT3/4 Antibody (50-5841-82) in Flow

Product Details

50-5841-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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