Invitrogen

OCT4 Polyclonal Antibody

OCT4 Antibody in Immunocytochemistry (ICC/IF)

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OCT4 Antibody (PA5-27438) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of OCT4 was performed in NT2D1 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: OCT4 Polyclonal Antibody (Product # PA5-27438) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

OCT4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

OCT4 Antibody in Immunoprecipitation (IP)

OCT4 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-27438

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:100,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 9 publications 9 publications

Flow Cytometry (Flow)

1:50-1:200

View 1 publication 1 publication

Immunoprecipitation (IP)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 360 of Human OCT3/4

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544914

Product Specific Information

Recommended positive controls: human ESC, mouse ESC, NT2D1.

Predicted reactivity: Mouse (83%), Rat (83%), Cat (94%), Pig (93%), Rabbit (90%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (91%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Correction of DMD in human iPSC-derived cardiomyocytes by base-editing-induced exon skipping.

Molecular therapy. Methods & clinical development

Wang P,Li H,Zhu M,Han RY,Guo S,Han R

PA5-27438 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to explore the feasibility of base-editing-mediated exon skipping as a therapeutic strategy for DMD using cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs).

Thu Mar 09 00:00:00 EST 2023

Correction of DMD in human iPSC-derived cardiomyocytes by base-editing-induced exon skipping.

Molecular therapy. Methods & clinical development

Wang P,Li H,Zhu M,Han RY,Guo S,Han R

Thu Mar 09 00:00:00 EST 2023

Differentiation-dependent changes in lamin B1 dynamics and lamin B receptor localization.

Molecular biology of the cell

Wesley CC,Levy DL

PA5-27438 was used in Immunohistochemistry and Western Blotting to suggest that differentiation-dependent tethering of lamin B1 either directly by LBR or indirectly via LBR-chromatin associations impacts gene expression.

Wed Feb 01 00:00:00 EST 2023

Differentiation-dependent changes in lamin B1 dynamics and lamin B receptor localization.

Molecular biology of the cell

Wesley CC,Levy DL

Wed Feb 01 00:00:00 EST 2023

Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.

Neuron

LaForce GR,Farr JS,Liu J,Akesson C,Gumus E,Pinkard O,Miranda HC,Johnson K,Sweet TJ,Ji P,Lin A,Coller J,Philippidou P,Wagner EJ,Schaffer AE

PA5-27438 was used in Immunocytochemistry-immunoflourescence to identify a previously uncharacterized role for CLP1 in mRNA 3' end formation and reveal an mRNA misprocessing signature in neurodegeneration that may suggest a common mechanism of disease.

Wed Apr 20 00:00:00 EDT 2022

Patient-specific, re-engineered cardiomyocyte model confirms the circumstance-dependent arrhythmia risk associated with the African-specific common SCN5A polymorphism p.S1103Y: Implications for the increased sudden deaths observed in black individuals during the COVID-19 pandemic.

Heart rhythm

Hamrick SK,John Kim CS,Tester DJ,Giudicessi JR,Ackerman MJ

PA5-27438 was used in Immunocytochemistry to ascertain the effects of acidosis and hydroxychloroquine (HCQ) on the action potential duration (APD) in a patient-specific induced pluripotent stem cell-derived cardiomyocyte (iPSC-CM) model of p.S1103Y-SCN5A.

Sun May 01 00:00:00 EDT 2022

Determining epigenetic memory in kidney proximal tubule cell derived induced pluripotent stem cells using a quadruple transgenic reprogrammable mouse.

Scientific reports

Khelifi G,Chow T,Whiteley J,Fort V,Humphreys BD,Hussein SMI,Rogers IM

Published figure using OCT4 polyclonal antibody (Product # PA5-27438) in Immunocytochemistry

Fri Nov 25 00:00:00 EST 2022

Determining epigenetic memory in kidney proximal tubule cell derived induced pluripotent stem cells using a quadruple transgenic reprogrammable mouse.

Scientific reports

Khelifi G,Chow T,Whiteley J,Fort V,Humphreys BD,Hussein SMI,Rogers IM

Published figure using OCT4 polyclonal antibody (Product # PA5-27438) in Immunocytochemistry

Fri Nov 25 00:00:00 EST 2022

Establishment of the iPSC line CUIMCi005-A from a patient with Stargardt disease for retinal organoid culture.

Stem cell research

Su PY,Lee W,Zernant J,Tsang SH,Nagasaki T,Corneo B,Allikmets R

PA5-27438 was used in Immunocytochemistry-immunofluorescence to establish an induced pluripotent stem cell line for retinal organoid research from a patient with mild disease features who is compound heterozygous for the frequent c.5882G>A (p.Gly1961Glu) missense variant and a c.4947delC (p.Glu1650Argfs*12) frameshift variant.

Thu Dec 01 00:00:00 EST 2022

Characterization of N-terminal RYR2 variants outside CPVT1 hotspot regions using patient iPSCs reveal pathogenesis and therapeutic potential.

Stem cell reports

Stutzman MJ,Kim CSJ,Tester DJ,Hamrick SK,Dotzler SM,Giudicessi JR,Miotto MC,Gc JB,Frank J,Marks AR,Ackerman MJ

PA5-27438 was used in Immunocytochemistry-immunoflourescence to show that these variants are located at the N-terminal domain and the central domain interface and could destabilize the RYR2 channel promoting Ca2+ leak-triggered arrhythmias.

Tue Sep 13 00:00:00 EDT 2022

In situ differentiation of human-induced pluripotent stem cells into functional cardiomyocytes on a coaxial PCL-gelatin nanofibrous scaffold.

Materials science & engineering. C, Materials for biological applications

Sridharan D,Palaniappan A,Blackstone BN,Dougherty JA,Kumar N,Seshagiri PB,Sayed N,Powell HM,Khan M

PA5-27438 was used in Immunocytochemistry to demonstrate that coaxial polycaprolactone-gelatin nanofibrous scaffolds can be used as a 3D culture platform for efficient differentiation of human-induced pluripotent stem cells to functional cardiomyocytes.

Fri Jan 01 00:00:00 EST 2021

Bone marrow-derived mesenchymal stem cells combined with gonadotropin therapy restore postnatal oogenesis of chemo-ablated ovaries in rats via enhancing very small embryonic-like stem cells.

Stem cell research & therapy

Ebrahim N,Al Saihati HA,Shaman A,Dessouky AA,Farid AS,Hussien NI,Mostafa O,Seleem Y,Sabry D,Saad AS,Emam HT,Hassouna A,Badr OAM,Saffaf BA,Forsyth NR,Salim RF

Published figure using OCT4 polyclonal antibody (Product # PA5-27438) in Immunohistochemistry

Mon Sep 27 00:00:00 EDT 2021

Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment.

Nature protocols

Tekel SJ,Brookhouser N,Standage-Beier K,Wang X,Brafman DA

PA5-27438 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to facilitate the highly efficient single-base editing of human cells at precise genomic loci.

Thu Jul 01 00:00:00 EDT 2021

The master transcription factor SOX2, mutated in anophthalmia/microphthalmia, is post-transcriptionally regulated by the conserved RNA-binding protein RBM24 in vertebrate eye development.

Human molecular genetics

Dash S,Brastrom LK,Patel SD,Scott CA,Slusarski DC,Lachke SA

PA5-27438 was used in Immunocytochemistry to investigate whether the conserved RNA-binding motif protein, RBM24, positively controls Sox2 mRNA stability and is necessary for optimal SOX2 mRNA and protein levels in development, perturbation of which causes ocular defects, including microphthalmia and anophthalmia.

Fri Mar 13 00:00:00 EDT 2020

Meagre Argyrosomus regius (Asso, 1801) Stem Spermatogonia: Histological Characterization, Immunostaining, In Vitro Proliferation, and Cryopreservation.

Animals : an open access journal from MDPI

Zupa R,Martino NA,Marzano G,Dell'Aquila ME,Corriero A

Published figure using OCT4 polyclonal antibody (Product # PA5-27438) in Immunohistochemistry

Thu May 14 00:00:00 EDT 2020

Meagre Argyrosomus regius (Asso, 1801) Stem Spermatogonia: Histological Characterization, Immunostaining, In Vitro Proliferation, and Cryopreservation.

Animals : an open access journal from MDPI

Zupa R,Martino NA,Marzano G,Dell'Aquila ME,Corriero A

Published figure using OCT4 polyclonal antibody (Product # PA5-27438) in Immunohistochemistry

Thu May 14 00:00:00 EDT 2020

Upregulation of stem cell markers ALDH1A1 and OCT4 as potential biomarkers for the early detection of cervical carcinoma.

Oncology letters

Tulake W,Yuemaier R,Sheng L,Ru M,Lidifu D,Abudula A

Published figure using OCT4 polyclonal antibody (Product # PA5-27438) in Immunohistochemistry (Paraffin)

Thu Nov 01 00:00:00 EDT 2018

The TDH-GCN5L1-Fbxo15-KBP axis limits mitochondrial biogenesis in mouse embryonic stem cells.

Nature cell biology

Donato V,Bonora M,Simoneschi D,Sartini D,Kudo Y,Saraf A,Florens L,Washburn MP,Stadtfeld M,Pinton P,Pagano M

Published figure using OCT4 polyclonal antibody (Product # PA5-27438) in Western Blot

Sat Apr 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2

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Gene Aliases: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1

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UniProt ID: (Human) Q01860, (Mouse) P20263

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Entrez Gene ID: (Human) 5460, (Mouse) 18999

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Function(s)

transcription regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding ubiquitin protein ligase binding miRNA binding sequence-specific DNA binding poly(A) RNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription coactivator activity chromatin binding transcription corepressor activity transcription factor binding chromatin DNA binding transcription regulatory region DNA binding protein heterodimerization activity POU domain binding HMG box domain binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter mesodermal cell fate commitment endodermal cell fate commitment ectodermal cell fate commitment endodermal cell fate specification trophectodermal cell differentiation blastocyst growth BMP signaling pathway involved in heart induction transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development response to organic substance regulation of gene expression stem cell population maintenance germ-line stem cell population maintenance response to retinoic acid somatic stem cell population maintenance positive regulation of catenin import into nucleus mRNA transcription from RNA polymerase II promoter cell fate commitment negative regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of calcium ion-dependent exocytosis stem cell differentiation positive regulation of protein kinase B signaling negative regulation of protein kinase B signaling positive regulation of SMAD protein import into nucleus cell fate commitment involved in formation of primary germ layer cardiac cell fate determination negative regulation of gene silencing by miRNA regulation of heart induction by regulation of canonical Wnt signaling pathway regulation of methylation-dependent chromatin silencing blastocyst development response to wounding anatomical structure morphogenesis regulation of asymmetric cell division

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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OCT4 Polyclonal Antibody

OCT4 Antibody (PA5-27438) in ICC/IF

Product Details

PA5-27438

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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