Invitrogen

OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

5 References

View all (71) OCT4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

OCT4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

OCT4 Antibody (701756) in ICC/IF

For immunofluorescence analysis, NTERA-2 cells were fixed and permeabilized for detection of endogenous OCT4 using Anti- OCT4 Recombinant Rabbit Monoclonal Antibody (Product # 701756, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of OCT4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S369... View More

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OCT4 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Expression of OCT4 was observed specifically in NTERA-2, NCCIT and F9, cell lines of embryonic origin, but not in the somatic tumor cell lines HEK-293, HeLa and A431, using Anti-OCT4 Recombinant Rabbit Monoclonal Antibody (Product # 701756) in Western blot. OCT4 is involved in the maintenance of embryonic stem cell pluripotency, and its expression is lost at the blastocyst stage. {RE}

Product Details

701756

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 3 publications 3 publications

ChIP assay (ChIP)

2.5 µg x 10^6 cells

in situ PLA (PLA)

- -

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

3H8L6

Immunogen

Protein corresponding to Human POU5F1 (aa 1-360)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2633031

Product Specific Information

This antibody is predicted to react with Monkey, Goat, Cat and Horse

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effects of DMSO on the Pluripotency of Cultured Mouse Embryonic Stem Cells (mESCs).

Stem cells international

Sousa MI,Correia B,Branco AF,Rodrigues AS,Ramalho-Santos J

Published figure using OCT4 recombinant monoclonal antibody (Product # 701756) in Western Blot

Sat Oct 31 00:00:00 EDT 2020

Effects of DMSO on the Pluripotency of Cultured Mouse Embryonic Stem Cells (mESCs).

Stem cells international

Sousa MI,Correia B,Branco AF,Rodrigues AS,Ramalho-Santos J

Published figure using OCT4 recombinant monoclonal antibody (Product # 701756) in Western Blot

Sat Oct 31 00:00:00 EDT 2020

Differentiation of Human Embryonic Stem Cells into Neuron, Cholinergic, and Glial Cells.

Stem cells international

Hosseini K,Lekholm E,Ahemaiti A,Fredriksson R

701756 was used in Immunocytochemistry to develop protocols for differentiation of hESCs into adherent monolayer hNSCs over a short period of time, with the possibility of both expansion and freezing.

Thu Dec 10 00:00:00 EST 2020

Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals.

Stem cell research

Xu X,Pradhan M,Xu M,Cheng YS,Beers J,Linask KL,Lin Y,Zheng W,Zou J

701756 was used in Immunocytochemistry-immunoflourescence to confirm the authenticity of established iPSC lines.

Tue Dec 01 00:00:00 EST 2020

Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals.

Stem cell research

Xu X,Pradhan M,Xu M,Cheng YS,Beers J,Linask KL,Lin Y,Zheng W,Zou J

701756 was used in Immunocytochemistry-immunoflourescence to confirm the authenticity of established iPSC lines.

Tue Dec 01 00:00:00 EST 2020

Dysfunction of iPSC-derived endothelial cells in human Hutchinson-Gilford progeria syndrome.

Cell cycle (Georgetown, Tex.)

Matrone G,Thandavarayan RA,Walther BK,Meng S,Mojiri A,Cooke JP

701756 was used in Western Blotting to investigate the dysfunction and differences in morphology of iPSC-derived endothelial cells extracted from HGPS patients compared to unaffected relatives.

Tue Oct 01 00:00:00 EDT 2019

Generation of two induced pluripotent stem cell lines (NHLBIi001-A and NHLBIi001-B) from a healthy Caucasian female volunteer with normal cardiac function.

Stem cell research

Patterson K,Beers J,Linask KL,Lin Y,Hassanzadeh S,Sack MN,Zou J

701756 was used in Immunocytochemistry-immunoflourescence to develop two wild-type human-derived induced pluripotent cell lines from a PBMC sample of a patient with normal cardiac function.

Sun Dec 01 00:00:00 EST 2019

Bioinformatics

Protein Aliases: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2

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Gene Aliases: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1

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UniProt ID: (Human) Q01860, (Mouse) P20263

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Entrez Gene ID: (Human) 5460, (Mouse) 18999

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Function(s)

transcription regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding ubiquitin protein ligase binding miRNA binding sequence-specific DNA binding poly(A) RNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription coactivator activity chromatin binding transcription corepressor activity transcription factor binding chromatin DNA binding transcription regulatory region DNA binding protein heterodimerization activity POU domain binding HMG box domain binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter mesodermal cell fate commitment endodermal cell fate commitment ectodermal cell fate commitment endodermal cell fate specification trophectodermal cell differentiation blastocyst growth BMP signaling pathway involved in heart induction transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development response to organic substance regulation of gene expression stem cell population maintenance germ-line stem cell population maintenance response to retinoic acid somatic stem cell population maintenance positive regulation of catenin import into nucleus mRNA transcription from RNA polymerase II promoter cell fate commitment negative regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of calcium ion-dependent exocytosis stem cell differentiation positive regulation of protein kinase B signaling negative regulation of protein kinase B signaling positive regulation of SMAD protein import into nucleus cell fate commitment involved in formation of primary germ layer cardiac cell fate determination negative regulation of gene silencing by miRNA regulation of heart induction by regulation of canonical Wnt signaling pathway regulation of methylation-dependent chromatin silencing blastocyst development response to wounding anatomical structure morphogenesis regulation of asymmetric cell division

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OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6)

OCT4 Antibody (701756) in ICC/IF

Product Details

701756

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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