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ODF2 Antibody (MA5-42829) in WB
Western blot analysis of ODF2 in extracts of various cell lines. Samples were incubated with ODF2 Monoclonal antibody (Product # MA5-42829) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-42829
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3Z1H8
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 730-829 of human Cenexin1 / ODF2 (Q5BJF6).
Conjugate
Form
Liquid
Concentration
0.99 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911970
Product Specific Information
Positive test controls include: HeLa, HepG2, PC-3.
Immunogen sequence: KEHALSKERA AQNKILDLET QLSRTKTELS QLRRSRDDAD RRYQSRLQDL KDRLEQSEST NRSMQNYVQF LKSSYANVFG DGPYSTFLTS SPIRSRSPPA
Target Information
Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cancer/testis antigen 134; Cenexin; cenexin 1; Outer dense fiber of sperm tails protein 2; outer dense fiber of sperm tails, 84-kD; Outer dense fiber protein 2; RP11-187B3.1; sperm tail structural protein; testis tissue sperm-binding protein Li 51e
Gene Aliases: CT134; ODF2; ODF2/1; ODF2/2; ODF84
UniProt ID: (Human) Q5BJF6
Entrez Gene ID: (Human) 4957
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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