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Invitrogen
OGDH Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 19
OGDH Antibody (PA5-28195) in ICC/IF
Immunofluorescence analysis of OGDH was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with OGDH Polyclonal Antibody (Product # PA5-28195) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More
Product Details
PA5-28195
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 705 and 1000 of Human OGDH
Conjugate
Form
Liquid
Concentration
0.3 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545671
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, Huh-7, Mouse brain.
Predicted reactivity: Mouse (93%), Rat (93%), Xenopus laevis (84%), Chicken (87%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitocondrial matrix and uses thiamine pyrophosphate as a cofactor. A congential deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2-oxoglutarate dehydrogenase complex component E1; 2-oxoglutarate dehydrogenase, mitochondrial; Alpha-ketoglutarate dehydrogenase; Alpha-KGDH-E1; E1o; OGDC-E1; oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide); oxoglutarate decarboxylase; oxoglutarate dehydrogenase (lipoamide); oxoglutarate dehydrogenase (succinyl-transferring); testicular tissue protein Li 131; Thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase
Gene Aliases: 2210403E04Rik; 2210412K19Rik; AA409584; AKGDH; d1401; E1k; Kiaa4192; mKIAA4192; OGDC; OGDH
UniProt ID: (Human) Q02218, (Mouse) Q60597
Entrez Gene ID: (Human) 4967, (Mouse) 18293
oxoglutarate dehydrogenase (succinyl-transferring) activity
thiamine pyrophosphate binding
heat shock protein binding
oxoglutarate dehydrogenase (NAD+) activity
metal ion binding
chaperone binding
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
dehydrogenase
generation of precursor metabolites and energy
glycolytic process
tricarboxylic acid cycle
2-oxoglutarate metabolic process
succinyl-CoA metabolic process
lysine catabolic process
NADH metabolic process
cerebellar cortex development
striatum development
hippocampus development
thalamus development
pyramidal neuron development
tangential migration from the subventricular zone to the olfactory bulb
glyoxylate metabolic process
olfactory bulb mitral cell layer development
metabolic process
oxidation-reduction process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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