Invitrogen

OGG1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

13 Published Figures

7 References

View all (16) OGG1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

OGG1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 19

OGG1 Antibody (PA1-31402) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of OGG1 in A431 cells. Cells were fixed with 10% formalin, permeabilized with 0.5% Triton-X100 and OGG1 Polyclonal Antibody (Product # PA1-31402) (Green) at a dilution of 1:200 was used as a primary antibody. Red : Tubulin. Blue: DAPI.

OGG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

OGG1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

OGG1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

OGG1 Antibody — OGG1 knockdown or inhibition sensitizes cancer cells to radiation (A) DLD-1 cells were transfected with three distinct siRNA against OGG1 or with a scramble RNA. OGG1 expression was monitored by RT-qPCR analysis and immunoblotting. (B) Cells were submitted to irradiation and their clonogenic efficiency was determined. Cloning efficiency of unexposed cells was set at 100%. Results of triplicate experiments are shown. Error bars represent standard error. ***p < 0.001; **p < 0.01.; *p < 0.05; Student's t-test. (C) Results from clonogenic efficiency data in Figure 7B are represented as line graphs, with all un-irradiated cells set at 100%. Results of triplicate experiments are shown. Error bars represent standard error. ***p < 0.001; **p < 0.01.; *p < 0.05; Student's t-test comparing each siRNA OGG1 against the scramble RNA control. 1: OGG1 siRNA 1; 2 : OGG1 siRNA 2; 3: OGG1 siRNA 3. (D) 8-oxoG cleavage assay was performed using 50 nM OGG1 and BSA in the presence of 10 µM. Chembridge 5552704 and 5245457 compounds or vehicle (DMSO). The two compounds inhibit the reaction. (E) DLD-1 colorectal cancer cells were irradiated (0 and 2 Gy) in the presence of 10 µM of Chembridge 5552704 and 5245457 compounds or vehicle (DMSO). Thymidine incorporation was measured for 3 days and is reported relative to the DMSO 0 h value. {KD}

Product Details

PA1-31402

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunohistochemistry (Frozen) (IHC (F))

1:25-1:100

Immunocytochemistry (ICC/IF)

1:100-1:500

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

1:10-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide derived from human Ogg1 (within amino acids 1-100) (UniProt# O15527)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2156790

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

8-hydroxyguanine, a form of oxidative DNA damage induced by free radicals, causes G:C to T:A transversion. In E. Coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine. One of these enzymes, MutM, was found to have a functional yeast (yOgg1) and human (hOgg1) homologue. hOgg1 proteins efficiently released the 8-hydroxyguanine opposite the pyrimidine from DNA and cleaved the AP site in a manner similar to bacterial and yeast enzymes. Genetic backgrounds in control of the repair of damaged DNA are involved in the susceptibility to cancer development. The hOgg1 gene has been mapped to region 3p26. 2, a region showing loss of heterozygosity (LOH) in a variety of cancers. In particular, 3p25-p26 is a common LOH region in lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Small-molecule-mediated OGG1 inhibition attenuates pulmonary inflammation and lung fibrosis in a murine lung fibrosis model.

Nature communications

Tanner L,Single AB,Bhongir RKV,Heusel M,Mohanty T,Karlsson CAQ,Pan L,Clausson CM,Bergwik J,Wang K,Andersson CK,Oommen RM,Erjefält JS,Malmström J,Wallner O,Boldogh I,Helleday T,Kalderén C,Egesten A

PA1-31402 was used in Immunohistochemistry and Western Blotting to suggest that TH5487 is a potentially clinically relevant treatment for IPF but further study in human trials is required.

Mon Feb 06 00:00:00 EST 2023

Small-molecule-mediated OGG1 inhibition attenuates pulmonary inflammation and lung fibrosis in a murine lung fibrosis model.

Nature communications

Tanner L,Single AB,Bhongir RKV,Heusel M,Mohanty T,Karlsson CAQ,Pan L,Clausson CM,Bergwik J,Wang K,Andersson CK,Oommen RM,Erjefält JS,Malmström J,Wallner O,Boldogh I,Helleday T,Kalderén C,Egesten A

PA1-31402 was used in Immunohistochemistry and Western Blotting to suggest that TH5487 is a potentially clinically relevant treatment for IPF but further study in human trials is required.

Mon Feb 06 00:00:00 EST 2023

Small-molecule-mediated OGG1 inhibition attenuates pulmonary inflammation and lung fibrosis in a murine lung fibrosis model.

Nature communications

Tanner L,Single AB,Bhongir RKV,Heusel M,Mohanty T,Karlsson CAQ,Pan L,Clausson CM,Bergwik J,Wang K,Andersson CK,Oommen RM,Erjefält JS,Malmström J,Wallner O,Boldogh I,Helleday T,Kalderén C,Egesten A

PA1-31402 was used in Immunohistochemistry and Western Blotting to suggest that TH5487 is a potentially clinically relevant treatment for IPF but further study in human trials is required.

Mon Feb 06 00:00:00 EST 2023

The DNA repair function of CUX1 contributes to radioresistance.

Oncotarget

Ramdzan ZM,Ginjala V,Pinder JB,Chung D,Donovan CM,Kaur S,Leduy L,Dellaire G,Ganesan S,Nepveu A

Published figure using OGG1 polyclonal antibody (Product # PA1-31402) in Western Blot

Tue Mar 21 00:00:00 EDT 2017

Special AT-rich Sequence-binding Protein 1 (SATB1) Functions as an Accessory Factor in Base Excision Repair.

The Journal of biological chemistry

Kaur S,Coulombe Y,Ramdzan ZM,Leduy L,Masson JY,Nepveu A

Published figure using OGG1 polyclonal antibody (Product # PA1-31402) in Western Blot

Fri Oct 21 00:00:00 EDT 2016

Special AT-rich Sequence-binding Protein 1 (SATB1) Functions as an Accessory Factor in Base Excision Repair.

The Journal of biological chemistry

Kaur S,Coulombe Y,Ramdzan ZM,Leduy L,Masson JY,Nepveu A

Published figure using OGG1 polyclonal antibody (Product # PA1-31402) in Western Blot

Fri Oct 21 00:00:00 EDT 2016

CUX2 protein functions as an accessory factor in the repair of oxidative DNA damage.

The Journal of biological chemistry

Pal R,Ramdzan ZM,Kaur S,Duquette PM,Marcotte R,Leduy L,Davoudi S,Lamarche-Vane N,Iulianella A,Nepveu A

PA1-31402 was used in western blot to analyze an accessory factor protein in oxidative DNA damage repair called CUX2

Fri Sep 11 00:00:00 EDT 2015

CUX2 protein functions as an accessory factor in the repair of oxidative DNA damage.

The Journal of biological chemistry

Pal R,Ramdzan ZM,Kaur S,Duquette PM,Marcotte R,Leduy L,Davoudi S,Lamarche-Vane N,Iulianella A,Nepveu A

PA1-31402 was used in western blot to analyze an accessory factor protein in oxidative DNA damage repair called CUX2

Fri Sep 11 00:00:00 EDT 2015

RAS transformation requires CUX1-dependent repair of oxidative DNA damage.

PLoS biology

Ramdzan ZM,Vadnais C,Pal R,Vandal G,Cadieux C,Leduy L,Davoudi S,Hulea L,Yao L,Karnezis AN,Paquet M,Dankort D,Nepveu A

Published figure using OGG1 polyclonal antibody (Product # PA1-31402) in Western Blot

Sat Mar 01 00:00:00 EST 2014

RAS transformation requires CUX1-dependent repair of oxidative DNA damage.

PLoS biology

Ramdzan ZM,Vadnais C,Pal R,Vandal G,Cadieux C,Leduy L,Davoudi S,Hulea L,Yao L,Karnezis AN,Paquet M,Dankort D,Nepveu A

Published figure using OGG1 polyclonal antibody (Product # PA1-31402) in Western Blot

Sat Mar 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: 8-hydroxyguanine DNA glycosylase; 8-oxoguanine DNA-glycosylase 1; 8-oxoguanine-DNA-glycosylase; AP lyase; DNA-apurinic or apyrimidinic site lyase; N-glycosylase/DNA lyase; OGG1 type 1f

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Gene Aliases: HMMH; HOGG1; MMH; MUTM; OGG1; OGH1

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UniProt ID: (Human) O15527, (Mouse) O08760, (Rat) O70249

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Entrez Gene ID: (Human) 4968, (Mouse) 18294, (Rat) 81528

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Function(s)

damaged DNA binding endonuclease activity protein binding microtubule binding oxidized purine nucleobase lesion DNA N-glycosylase activity oxidized purine DNA binding 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity purine-specific mismatch base pair DNA N-glycosylase activity oxidized base lesion DNA N-glycosylase activity DNA binding catalytic activity DNA-(apurinic or apyrimidinic site) lyase activity uracil DNA N-glycosylase activity hydrolase activity hydrolase activity, acting on glycosyl bonds lyase activity single-strand selective uracil DNA N-glycosylase activity DNA N-glycosylase activity DNA-3-methylbase glycosylase activity nicotinamide riboside hydrolase activity nicotinic acid riboside hydrolase activity deoxyribonucleoside 5'-monophosphate N-glycosidase activity DNA metabolism protein

Process(es)

acute inflammatory response base-excision repair regulation of transcription, DNA-templated cellular response to DNA damage stimulus response to oxidative stress aging response to radiation response to light stimulus response to estradiol regulation of protein import into nucleus, translocation nucleotide-excision repair, DNA incision response to drug negative regulation of apoptotic process depurination depyrimidination response to ethanol response to folic acid cellular response to cadmium ion negative regulation of double-strand break repair via single-strand annealing DNA repair nucleotide-excision repair metabolic process nucleic acid phosphodiester bond hydrolysis DNA catabolic process, endonucleolytic

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OGG1 Polyclonal Antibody

OGG1 Antibody (PA1-31402) in ICC/IF

Product Details

PA1-31402

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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