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OLFM4 Antibody (PA5-116619) in WB
Western blot analysis of OLFM4 in extracts of various cell lines. Samples were incubated with OLFM4 Polyclonal antibody (Product # PA5-116619) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
PA5-116619
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 211-510 of human OLFM4 (NP_0064093)
Conjugate
Form
Liquid
Concentration
6.18 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2901250
Product Specific Information
Positive Samples: PC-3, Mouse spleen, Mouse stomach, Rat stomach
Immunogen sequence: AIRREIVALK TKLKECEASK DQNTPVVHPP PTPGSCGHGG VVNISKPSVV QLNWRGFSYL YGAWGRDYSP QHPNKGLYWV APLNTDGRLL EYYRLYNTLD DLLLYINARE LRITYGQGSG TAVYNNNMYV NMYNTGNIAR VNLTTNTIAV TQTLPNAAYN NRFSYANVAW QDIDFAVDEN GLWVIYSTEA STGNMVISKL NDTTLQVLNT WYTKQYKPSA SNAFMVCGVL YATRTMNTRT EEIFYYYDTN TGKEGKLDIV MHKMQEKVQS INYNPFDQKL YVYNDGYLLN YDLSVLQKPQ
Target Information
OLFM4 is a 510 amino acid extracellular matrix glycoprotein belonging to the olfactomedin-related protein family and found to be selectively expressed in inflammed colonic epithelium. It has one signal peptide, six N-linked glycosylation motif and forms disulfide-bonded multimers. OLFM4 a transcription factor may have a matrix-related function in differentiation. Constitutively expressed OLFM4 in cancer cells binds to the potent apoptosis inducer GRIM-19 and has a novel antiapoptotic action and hence might serve as a diagnostic marker for human cancers. Reports suggest that OLFM4 promotes proliferation of PANC-1 cells by favoring transition from the S to G2/M phase. Potential interaction of OLFM4 with endogenous cell surface lectins and cadherins may mediate cell adhesion.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antiapoptotic protein GW112; G-CSF-stimulated clone 1 protein; hGC-1; hOLfD; Olfactomedin-4; OLM4; PU.1 difference product 4
Gene Aliases: bA209J19.1; GC1; Gm296; Gm913; GW112; hGC-1; hOLfD; OlfD; OLFM4; OLM4; pPD4; UNQ362; UNQ362/PRO698
UniProt ID: (Human) Q6UX06
Entrez Gene ID: (Human) 10562, (Mouse) 380924, (Rat) 290409
cell adhesion
regulation of necrotic cell death
regulation of apoptotic process
regulation of phagocytosis
protein homooligomerization
positive regulation of substrate adhesion-dependent cell spreading
regulation of neutrophil extravasation
negative regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of immune response
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