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OLIG2 Antibody (MA5-35533) in WB
Western blot analysis of OLIG2 in extracts of various cell lines. Samples were incubated with OLIG2 Monoclonal antibody (Product # MA5-35533) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Product Details
MA5-35533
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC1032
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Olig2 (Q13516)
Conjugate
Form
Liquid
Concentration
0.43 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849433
Product Specific Information
Immunogen sequence: AATAHPAAAA HAAHHPAVHH PILPPAAAAA AAAAAAAAVS SASLPGSGLP SVGSIRPPHG LLKSPSAAAA APLGGGGGGS GASGGFQHWG GMPCPCSMCQ V
Target Information
This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. Tissue specificity: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Basic helix loop helix protein class B1 (bHLHB1); basic helix-loop-helix protein 19 (bHLHe19); olig-2; Oligo2; Oligo2 antibody; Oligodendrocyte specific bHLH transcription factor 2; Oligodendrocyte transcription factor 2; Protein kinase C-binding protein 2 (PRKCBP2)
Gene Aliases: AI604895; Bhlhb1; bHLHe19; Olg-2; Olig2; Oligo2; RK17
UniProt ID: (Mouse) Q9EQW6
Entrez Gene ID: (Mouse) 50913, (Rat) 304103
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
protein homodimerization activity
protein dimerization activity
HMG box domain binding
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
nervous system development
spinal cord motor neuron differentiation
spinal cord oligodendrocyte cell differentiation
spinal cord oligodendrocyte cell fate specification
thalamus development
myelination
negative regulation of neuron differentiation
neuron fate commitment
oligodendrocyte differentiation
positive regulation of oligodendrocyte differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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