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OTUB1 Antibody (PA5-120082) in WB
Western blot analysis of OTUB1 in extracts of various cell lines. Samples were incubated with OTUB1 Polyclonal antibody (Product # PA5-120082) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-120082
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human OTUB1 (NP_060140.2).
Conjugate
Form
Liquid
Concentration
0.09 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913654
Product Specific Information
Positive test controls include: 293T, Mouse intestine, Mouse liver. The target is usually found in the following locations: Cytoplasm.
Immunogen sequence: ERLELSVLYK EYAEDDNIYQ QKIKDLHKKY SYIRKTRPDG NCFYRAFGFS HLEALLDDSK ELQRFKAVSA KSKEDLVSQG FTEFTIEDFH NTFMDLIEQV E
Target Information
The product of this gene is a member of the OTU superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme OTUB1; FLJ20113; FLJ40710; hOTU1; MGC111158; MGC4584; OTU domain, ubiquitin aldehyde binding 1; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTU-domain Ubal-binding 1; otubain 1; Otubain-1; Ubiquitin thioesterase OTUB1; ubiquitin-specific protease otubain 1; Ubiquitin-specific-processing protease OTUB1
Gene Aliases: AI850305; HSPC263; OTB1; OTU1; OTUB1
UniProt ID: (Human) Q96FW1, (Mouse) Q7TQI3
Entrez Gene ID: (Human) 55611, (Mouse) 107260
adaptive immune response
DNA repair
cellular response to DNA damage stimulus
protein K48-linked deubiquitination
cellular response to interleukin-1
negative regulation of histone H2A K63-linked ubiquitination
negative regulation of double-strand break repair
immune system process
proteolysis
protein deubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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