Bioss

OTX1 + OTX2 Polyclonal Antibody

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

BS-11958R

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-

ELISA (ELISA)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human OTX1 + OTX2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Transcription factors, OTX1 and OTX2, are two murine homologs of the Drosophila orthodenticle (OTD), show a limited amino acid sequence divergence. OTX1 and OTX2 play an important role during early and later events required for proper brain development in that they are involved in the processes of induction, specification and regionalization of the brain. OTX1 is involved in corticogenesis, sensory organ development and pituitary functions, while OTX2 is necessary earlier in development, for the correct anterior neural plate specification and organization of the primitive streak. OTX2 is also required in the early specification of the neuroectoderm, which is destined to become the fore-midbrain, and both OTX1 and OTX2 co-operate in patterning the developing brain through a dosage-dependent mechanism. A molecular mechanism depending on a precise threshold of OTX proteins is necessary for the correct positioning of the isthmic region and for anterior brain patterning. The genes which encode OTX1 and OTX2 map to human chromosomes 2p13 and 14q21-q22, respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: homeobox Otx2; Homeobox protein OTX1; Homeobox protein OTX2; Orthodenticle homolog 1; Orthodenticle homolog 2

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Gene Aliases: A730044F23Rik; CPHD6; E130306E05Rik; jv; MCOPS5; Otx-1; OTX1; OTX1X; OTX2

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UniProt ID: (Human) P32242, (Human) P32243, (Mouse) P80205, (Rat) Q63410

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Entrez Gene ID: (Human) 5013, (Human) 5015, (Mouse) 18423, (Rat) 25646, (Rat) 305858, (Mouse) 18424

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding eukaryotic initiation factor 4E binding DNA binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding protein heterodimerization activity homeodomain transcription factor
Process(es)
transcription from RNA polymerase II promoter anterior/posterior pattern specification metencephalon development midbrain development inner ear morphogenesis positive regulation of transcription from RNA polymerase II promoter diencephalon morphogenesis protein complex assembly axon guidance regulation of smoothened signaling pathway forebrain development positive regulation of embryonic development regulation of fibroblast growth factor receptor signaling pathway positive regulation of transcription, DNA-templated dopaminergic neuron differentiation primitive streak formation positive regulation of gastrulation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter multicellular organism development cell fate specification central nervous system development endoderm development organ morphogenesis dorsal/ventral pattern formation cell differentiation neuron differentiation somite rostral/caudal axis specification eye photoreceptor cell fate commitment cell fate commitment neuron fate commitment neuron fate determination anatomical structure development
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