Proteintech

OTX2 Polyclonal Antibody

OTX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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OTX2 Antibody (13497-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human gliomas using 13497-1-AP (OTX2 antibody) at dilution of 1:100 (under 10x lens).

OTX2 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

OTX2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13497-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 7 publications 7 publications

Flow Cytometry (Flow)

1:100

Immunoprecipitation (IP)

0.5-4.0 µg

ChIP assay (ChIP)

Assay-dependent

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

OTX2 Fusion Protein Ag4323 (1-297 aa encoded by BC032579)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.61 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MMSYLKQPP YAVNGLSLTT SGMDLLHPSV GYPGPWASCP AATPRKQRRE RTTFTRAQLD VLEALFAKTR YPDIFMREEV ALKINLPESR VQVWFKNRRA KCRQQQQQQQ NGGQNKVRPA KKKTSPAREV SSESGTSGQF TPPSSTSVPT IASSSAPVSI WSPASISPLS DPLSTSSSCM QRSYPMTYTQ ASGYSQGYAG STSYFGGMDC GSYLTPMHHQ LPGPGATLSP MGTNAVTSHL NQSPASLSTQ GYGASSLGFN STTDCLDYKD QTASWKLNFN ADCLDYKDQT SSWKFQVL (1-297 aa encoded by BC032579)

Target Information

OTX2 is a 289 amino acid containing protein encoding a member of the bicoid sub-family of homeodomain-containing transcription factors. It has two known alternative splice variants with distinct isoforms though presence of other isoforms cannot be excluded. The protein is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sensory organs. The protein may bind to BCD target sequence (BTS): 5-'TCTAATCCC-3'. Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

AAV-mediated gene augmentation therapy of CRB1 patient-derived retinal organoids restores the histological and transcriptional retinal phenotype.

Stem cell reports

Boon N,Lu X,Andriessen CA,Moustakas I,Buck TM,Freund C,Arendzen CH,Böhringer S,Mei H,Wijnholds J

Tue May 09 00:00:00 EDT 2023

Dissecting super-enhancer driven transcriptional dependencies reveals novel therapeutic strategies and targets for group 3 subtype medulloblastoma.

Journal of experimental & clinical cancer research : CR

Li M,Han Y,Wang C,Kang W,Jiang W,Zhang L,Tang Y

Sat Oct 22 00:00:00 EDT 2022

Dissecting super-enhancer driven transcriptional dependencies reveals novel therapeutic strategies and targets for group 3 subtype medulloblastoma.

Journal of experimental & clinical cancer research : CR

Li M,Han Y,Wang C,Kang W,Jiang W,Zhang L,Tang Y

Sat Oct 22 00:00:00 EDT 2022

Single-cell transcriptomic landscapes of the otic neuronal lineage at multiple early embryonic ages.

Cell reports

Sun Y,Wang L,Zhu T,Wu B,Wang G,Luo Z,Li C,Wei W,Liu Z

Tue Mar 22 00:00:00 EDT 2022

Unified rhombic lip origins of group 3 and group 4 medulloblastoma.

Nature

Smith KS,Bihannic L,Gudenas BL,Haldipur P,Tao R,Gao Q,Li Y,Aldinger KA,Iskusnykh IY,Chizhikov VV,Scoggins M,Zhang S,Edwards A,Deng M,Glass IA,Overman LM,Millman J,Sjoboen AH,Hadley J,Golser J,Mankad K,Sheppard H,Onar-Thomas A,Gajjar A,Robinson GW,Hovestadt V,Orr BA,Patay Z,Millen KJ,Northcott PA

Thu Sep 01 00:00:00 EDT 2022

Elimination of senescent cells inhibits epithelial-mesenchymal transition of retinal pigment epithelial cells.

Experimental eye research

Gao F,Wang L,Wu B,Ou Q,Tian H,Xu J,Jin C,Zhang J,Wang J,Lu L,Xu GT

Sat Oct 01 00:00:00 EDT 2022

Exogenous Otx2 protects midbrain dopaminergic neurons from MPP⁺ by interacting with ATP5a1 and promoting ATP synthesis.

Neurotoxicology

Xue J,Li Y,Qi Y,Zhang Z,Tang X

Fri Jul 01 00:00:00 EDT 2022

Binge-Like Ethanol Drinking Increases Otx2, Wnt1, and Mdk Gene Expression in the Ventral Tegmental Area of Adult Mice.

Neuroscience insights

Coles C,Lasek AW

Sun May 09 00:00:00 EDT 2021

Binge-Like Ethanol Drinking Increases Otx2, Wnt1, and Mdk Gene Expression in the Ventral Tegmental Area of Adult Mice.

Neuroscience insights

Coles C,Lasek AW

Sun May 09 00:00:00 EDT 2021

OTX2 regulates CFTR expression during endoderm differentiation and occupies 3' cis-regulatory elements.

Developmental dynamics : an official publication of the American Association of Anatomists

Kerschner JL,Paranjapye A,NandyMazumdar M,Yin S,Leir SH,Harris A

Sat May 01 00:00:00 EDT 2021

OTX2 regulates CFTR expression during endoderm differentiation and occupies 3' cis-regulatory elements.

Developmental dynamics : an official publication of the American Association of Anatomists

Kerschner JL,Paranjapye A,NandyMazumdar M,Yin S,Leir SH,Harris A

Sat May 01 00:00:00 EDT 2021

Cis-regulatory dissection of cone development reveals a broad role for Otx2 and Oc transcription factors.

Development (Cambridge, England)

Lonfat N,Wang S,Lee C,Garcia M,Choi J,Park PJ,Cepko C

Sat May 01 00:00:00 EDT 2021

Sox6 expression distinguishes dorsally and ventrally biased dopamine neurons in the substantia nigra with distinctive properties and embryonic origins.

Cell reports

Pereira Luppi M,Azcorra M,Caronia-Brown G,Poulin JF,Gaertner Z,Gatica S,Moreno-Ramos OA,Nouri N,Dubois M,Ma YC,Ramakrishnan C,Fenno L,Kim YS,Deisseroth K,Cicchetti F,Dombeck DA,Awatramani R

Tue Nov 09 00:00:00 EST 2021

Nuclear NAD⁺-biosynthetic enzyme NMNAT1 facilitates development and early survival of retinal neurons.

eLife

Sokolov D,Sechrest ER,Wang Y,Nevin C,Du J,Kolandaivelu S

Wed Dec 08 00:00:00 EST 2021

A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation.

eLife

Wang J,Rattner A,Nathans J

Tue Nov 16 00:00:00 EST 2021

Effective Inhibition of MYC-Amplified Group 3 Medulloblastoma Through Targeting EIF4A1.

Cancer management and research

Zhao Y,Li T,Tian S,Meng W,Sui Y,Yang J,Wang B,Liang Z,Zhao H,Han Y,Tang Y,Zhang L,Ma J

Mon Apr 18 00:00:00 EDT 2022

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Nature

Qian H,Kang X,Hu J,Zhang D,Liang Z,Meng F,Zhang X,Xue Y,Maimon R,Dowdy SF,Devaraj NK,Zhou Z,Mobley WC,Cleveland DW,Fu XD

Mon Jun 01 00:00:00 EDT 2020

Molecular network of miR-1343 regulates the pluripotency of porcine pluripotent stem cells via repressing OTX2 expression.

RNA biology

Xie Y,Cao H,Zhang Z,Zhang S,Wang H

Tue Jan 01 00:00:00 EST 2019

Molecular network of miR-1343 regulates the pluripotency of porcine pluripotent stem cells via repressing OTX2 expression.

RNA biology

Xie Y,Cao H,Zhang Z,Zhang S,Wang H

Tue Jan 01 00:00:00 EST 2019

ZIC3 Controls the Transition from Naive to Primed Pluripotency.

Cell reports

Yang SH,Andrabi M,Biss R,Murtuza Baker S,Iqbal M,Sharrocks AD

Tue Jun 11 00:00:00 EDT 2019

DMBX1 promotes tumor proliferation and regulates cell cycle progression via repressing OTX2-mediated transcription of p21 in lung adenocarcinoma cell.

Cancer letters

Luo J,Liu K,Yao Y,Sun Q,Zheng X,Zhu B,Zhang Q,Xu L,Shen Y,Ren B

Mon Jul 01 00:00:00 EDT 2019

Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex.

Neuropharmacology

Li Y,Wang L,Zhang X,Huang M,Li S,Wang X,Chen L,Jiang B,Yang Y

Mon Jan 01 00:00:00 EST 2018

Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex.

Neuropharmacology

Li Y,Wang L,Zhang X,Huang M,Li S,Wang X,Chen L,Jiang B,Yang Y

Mon Jan 01 00:00:00 EST 2018

Human Clinical-Grade Parthenogenetic ESC-Derived Dopaminergic Neurons Recover Locomotive Defects of Nonhuman Primate Models of Parkinson's Disease.

Stem cell reports

Wang YK,Zhu WW,Wu MH,Wu YH,Liu ZX,Liang LM,Sheng C,Hao J,Wang L,Li W,Zhou Q,Hu BY

Tue Jul 10 00:00:00 EDT 2018

Human Clinical-Grade Parthenogenetic ESC-Derived Dopaminergic Neurons Recover Locomotive Defects of Nonhuman Primate Models of Parkinson's Disease.

Stem cell reports

Wang YK,Zhu WW,Wu MH,Wu YH,Liu ZX,Liang LM,Sheng C,Hao J,Wang L,Li W,Zhou Q,Hu BY

Tue Jul 10 00:00:00 EDT 2018

Early life stress confers lifelong stress susceptibility in mice via ventral tegmental area OTX2.

Science (New York, N.Y.)

Peña CJ,Kronman HG,Walker DM,Cates HM,Bagot RC,Purushothaman I,Issler O,Loh YE,Leong T,Kiraly DD,Goodman E,Neve RL,Shen L,Nestler EJ

Fri Jun 16 00:00:00 EDT 2017

Early life stress confers lifelong stress susceptibility in mice via ventral tegmental area OTX2.

Science (New York, N.Y.)

Peña CJ,Kronman HG,Walker DM,Cates HM,Bagot RC,Purushothaman I,Issler O,Loh YE,Leong T,Kiraly DD,Goodman E,Neve RL,Shen L,Nestler EJ

Fri Jun 16 00:00:00 EDT 2017

Early life stress confers lifelong stress susceptibility in mice via ventral tegmental area OTX2.

Science (New York, N.Y.)

Peña CJ,Kronman HG,Walker DM,Cates HM,Bagot RC,Purushothaman I,Issler O,Loh YE,Leong T,Kiraly DD,Goodman E,Neve RL,Shen L,Nestler EJ

Fri Jun 16 00:00:00 EDT 2017

Early life stress confers lifelong stress susceptibility in mice via ventral tegmental area OTX2.

Science (New York, N.Y.)

Peña CJ,Kronman HG,Walker DM,Cates HM,Bagot RC,Purushothaman I,Issler O,Loh YE,Leong T,Kiraly DD,Goodman E,Neve RL,Shen L,Nestler EJ

Fri Jun 16 00:00:00 EDT 2017

Atypical Teratoid/Rhabdoid Tumors Are Comprised of Three Epigenetic Subgroups with Distinct Enhancer Landscapes.

Cancer cell

Johann PD,Erkek S,Zapatka M,Kerl K,Buchhalter I,Hovestadt V,Jones DTW,Sturm D,Hermann C,Segura Wang M,Korshunov A,Rhyzova M,Gröbner S,Brabetz S,Chavez L,Bens S,Gröschel S,Kratochwil F,Wittmann A,Sieber L,Geörg C,Wolf S,Beck K,Oyen F,Capper D,van Sluis P,Volckmann R,Koster J,Versteeg R,von Deimling A,Milde T,Witt O,Kulozik AE,Ebinger M,Shalaby T,Grotzer M,Sumerauer D,Zamecnik J,Mora J,Jabado N,Taylor MD,Huang A,Aronica E,Bertoni A,Radlwimmer B,Pietsch T,Schüller U,Schneppenheim R,Northcott PA,Korbel JO,Siebert R,Frühwald MC,Lichter P,Eils R,Gajjar A,Hasselblatt M,Pfister SM,Kool M

Mon Mar 14 00:00:00 EDT 2016

Atypical Teratoid/Rhabdoid Tumors Are Comprised of Three Epigenetic Subgroups with Distinct Enhancer Landscapes.

Cancer cell

Mon Mar 14 00:00:00 EDT 2016

Otx2 and Oct4 drive early enhancer activation during embryonic stem cell transition from naive pluripotency.

Cell reports

Yang SH,Kalkan T,Morissroe C,Marks H,Stunnenberg H,Smith A,Sharrocks AD

Thu Jun 26 00:00:00 EDT 2014

Otx2 and Oct4 drive early enhancer activation during embryonic stem cell transition from naive pluripotency.

Cell reports

Yang SH,Kalkan T,Morissroe C,Marks H,Stunnenberg H,Smith A,Sharrocks AD

Thu Jun 26 00:00:00 EDT 2014

Otx2 and Oct4 drive early enhancer activation during embryonic stem cell transition from naive pluripotency.

Cell reports

Yang SH,Kalkan T,Morissroe C,Marks H,Stunnenberg H,Smith A,Sharrocks AD

Thu Jun 26 00:00:00 EDT 2014

Otx2 and Oct4 drive early enhancer activation during embryonic stem cell transition from naive pluripotency.

Cell reports

Yang SH,Kalkan T,Morissroe C,Marks H,Stunnenberg H,Smith A,Sharrocks AD

Thu Jun 26 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: homeobox Otx2; Homeobox protein OTX2; Orthodenticle homolog 2; OTX

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Gene Aliases: CPHD6; E130306E05Rik; MCOPS5; OTX2

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UniProt ID: (Human) P32243

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Entrez Gene ID: (Human) 5015, (Rat) 305858, (Mouse) 18424

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding eukaryotic initiation factor 4E binding DNA binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding protein heterodimerization activity homeodomain transcription factor

Process(es)

transcription from RNA polymerase II promoter protein complex assembly axon guidance regulation of smoothened signaling pathway forebrain development midbrain development positive regulation of embryonic development regulation of fibroblast growth factor receptor signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter dopaminergic neuron differentiation primitive streak formation positive regulation of gastrulation regulation of transcription, DNA-templated multicellular organism development cell fate specification regulation of transcription from RNA polymerase II promoter central nervous system development endoderm development organ morphogenesis anterior/posterior pattern specification dorsal/ventral pattern formation metencephalon development cell differentiation neuron differentiation somite rostral/caudal axis specification inner ear morphogenesis eye photoreceptor cell fate commitment cell fate commitment neuron fate commitment neuron fate determination diencephalon morphogenesis anatomical structure development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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OTX2 Polyclonal Antibody

OTX2 Antibody (13497-1-AP) in IHC (P)

Product Details

13497-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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