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OTX2 Antibody (PA5-115699) in IHC (P)
Immunohistochemistry analysis of OTX2 in rat brain tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115699) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.
Product Details
PA5-115699
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human OTX2(Accession P32243), corresponding to amino acid residues D200-F250.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900334
Product Specific Information
Antibody detects endogenous levels of total Otx2.
Target Information
OTX2 is a 289 amino acid containing protein encoding a member of the bicoid sub-family of homeodomain-containing transcription factors. It has two known alternative splice variants with distinct isoforms though presence of other isoforms cannot be excluded. The protein is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sensory organs. The protein may bind to BCD target sequence (BTS): 5-'TCTAATCCC-3'. Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: homeobox Otx2; Homeobox protein OTX2; Orthodenticle homolog 2; OTX
Gene Aliases: CPHD6; E130306E05Rik; MCOPS5; OTX2
UniProt ID: (Human) P32243
Entrez Gene ID: (Human) 5015, (Rat) 305858, (Mouse) 18424
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
eukaryotic initiation factor 4E binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
protein heterodimerization activity
homeodomain transcription factor
transcription from RNA polymerase II promoter
protein complex assembly
axon guidance
regulation of smoothened signaling pathway
forebrain development
midbrain development
positive regulation of embryonic development
regulation of fibroblast growth factor receptor signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
dopaminergic neuron differentiation
primitive streak formation
positive regulation of gastrulation
regulation of transcription, DNA-templated
multicellular organism development
cell fate specification
regulation of transcription from RNA polymerase II promoter
central nervous system development
endoderm development
organ morphogenesis
anterior/posterior pattern specification
dorsal/ventral pattern formation
metencephalon development
cell differentiation
neuron differentiation
somite rostral/caudal axis specification
inner ear morphogenesis
eye photoreceptor cell fate commitment
cell fate commitment
neuron fate commitment
neuron fate determination
diencephalon morphogenesis
anatomical structure development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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