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OTX2 Antibody (CF809511) in IHC (P)
Immunohistochemical staining of paraffin-embedded human embryonic brain cortex tissue within the normal limits using anti-OTX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809511)(1:250)
Product Details
CF809511
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1A1
Immunogen
Full length human recombinant protein of human OTX2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
OTX2 is a 289 amino acid containing protein encoding a member of the bicoid sub-family of homeodomain-containing transcription factors. It has two known alternative splice variants with distinct isoforms though presence of other isoforms cannot be excluded. The protein is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sensory organs. The protein may bind to BCD target sequence (BTS): 5-'TCTAATCCC-3'. Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: homeobox Otx2; Homeobox protein OTX2; Orthodenticle homolog 2; OTX
Gene Aliases: CPHD6; E130306E05Rik; MCOPS5; OTX2
UniProt ID: (Human) P32243
Entrez Gene ID: (Human) 5015, (Rat) 305858, (Mouse) 18424
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
eukaryotic initiation factor 4E binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
protein heterodimerization activity
homeodomain transcription factor
transcription from RNA polymerase II promoter
protein complex assembly
axon guidance
regulation of smoothened signaling pathway
forebrain development
midbrain development
positive regulation of embryonic development
regulation of fibroblast growth factor receptor signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
dopaminergic neuron differentiation
primitive streak formation
positive regulation of gastrulation
regulation of transcription, DNA-templated
multicellular organism development
cell fate specification
regulation of transcription from RNA polymerase II promoter
central nervous system development
endoderm development
organ morphogenesis
anterior/posterior pattern specification
dorsal/ventral pattern formation
metencephalon development
cell differentiation
neuron differentiation
somite rostral/caudal axis specification
inner ear morphogenesis
eye photoreceptor cell fate commitment
cell fate commitment
neuron fate commitment
neuron fate determination
diencephalon morphogenesis
anatomical structure development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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