Invitrogen

Occludin Polyclonal Antibody

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

13 Published Figures

14 References

View all (58) Occludin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Occludin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

Occludin Antibody (42-2400) in ICC/IF

Immunofluorescent analysis of occludin Antibody was done on 90% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Occludin Antibody (Product # 42-2400) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 45 minutes at room... View More

Occludin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Occludin Antibody in Immunohistochemistry (IHC)

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Product Details

42-2400

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human

Published species

Bacteria, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Raised against the C-term of human Occludin.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533516

Target Information

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fucose ameliorates the proinflammatory property of Fusobacterium nucleatum in colitis via altering its metabolism.

Frontiers in cellular and infection microbiology

Duan C,Hou L,Deng X,Wu J,Qian W,Han C,Hou X

42-2400 was used in Western Blot to explore whether fucose could ameliorate the proinflammatory property of Fn in colitis and the underlying mechanisms.

Fri May 19 00:00:00 EDT 2023

Fucose ameliorates the proinflammatory property of Fusobacterium nucleatum in colitis via altering its metabolism.

Frontiers in cellular and infection microbiology

Duan C,Hou L,Deng X,Wu J,Qian W,Han C,Hou X

42-2400 was used in Western Blot to explore whether fucose could ameliorate the proinflammatory property of Fn in colitis and the underlying mechanisms.

Fri May 19 00:00:00 EDT 2023

Lactic Acid Bacteria Strains Used as Starters for Kimchi Fermentation Protect the Disruption of Tight Junctions in the Caco-2 Cell Monolayer Model.

Journal of microbiology and biotechnology

Kang JY,Lee M,Song JH,Choi EJ,Kim DU,Lim SK,Kim N,Chang JY

42-2400 was used in Immunocytochemistry to investigate the effect of lactic acid bacteria (LAB) strains used as starters for kimchi fermentation, namely Lactococcus lactis WiKim0124, Companilactobacillus allii WiKim39, Leuconostoc mesenteroides WiKim0121 Leuconostoc mesenteroides WiKim33, and Leuconostoc mesenteroides WiKim32, on the intestinal epithelial tight junctions (TJs).

Wed Dec 28 00:00:00 EST 2022

Evaluation of blood-testis barrier integrity in terms of adhesion molecules in nonobstructive azoospermia.

Andrologia

Aydin S,Billur D,Kizil S,Ozkavukcu S,Topal Celikkan F,Aydos K,Erdemli E

Published figure using Occludin polyclonal antibody (Product # 42-2400) in Immunohistochemistry

Sat Aug 01 00:00:00 EDT 2020

Campylobacter jejuni enters gut epithelial cells and impairs intestinal barrier function through cleavage of occludin by serine protease HtrA.

Gut pathogens

Harrer A,Bücker R,Boehm M,Zarzecka U,Tegtmeyer N,Sticht H,Schulzke JD,Backert S

42-2400 was used in Immunocytochemistry-immunoflourescence to investigate the interaction of high temperature requirement protein A with the host protein occludin, a tight junction strand component.

Thu Oct 01 00:00:00 EDT 2020

Campylobacter jejuni enters gut epithelial cells and impairs intestinal barrier function through cleavage of occludin by serine protease HtrA.

Gut pathogens

Harrer A,Bücker R,Boehm M,Zarzecka U,Tegtmeyer N,Sticht H,Schulzke JD,Backert S

42-2400 was used in Immunocytochemistry-immunoflourescence to investigate the interaction of high temperature requirement protein A with the host protein occludin, a tight junction strand component.

Thu Oct 01 00:00:00 EDT 2020

Helicobacter pylori Employs a Unique Basolateral Type IV Secretion Mechanism for CagA Delivery.

Cell host & microbe

Tegtmeyer N,Wessler S,Necchi V,Rohde M,Harrer A,Rau TT,Asche CI,Boehm M,Loessner H,Figueiredo C,Naumann M,Palmisano R,Solcia E,Ricci V,Backert S

42-2400 was used in Western Blotting to demonstrate that type IV secretion system pilus formation during infection of polarised cells occurs predominantly at basolateral membranes, and not at apical sites.

Wed Oct 11 00:00:00 EDT 2017

Heterogeneous vascular permeability and alternative diffusion barrier in sensory circumventricular organs of adult mouse brain.

Cell and tissue research

Morita S,Furube E,Mannari T,Okuda H,Tatsumi K,Wanaka A,Miyata S

42-2400 was used in immunohistochemistry (free floating) to assess the vascular permeability of blood-derived molecules and the expression of tight-junction proteins in sensory circumventricular organs.

Mon Feb 01 00:00:00 EST 2016

Matrix metalloproteinase 13 modulates intestinal epithelial barrier integrity in inflammatory diseases by activating TNF.

EMBO molecular medicine

Vandenbroucke RE,Dejonckheere E,Van Hauwermeiren F,Lodens S,De Rycke R,Van Wonterghem E,Staes A,Gevaert K,López-Otin C,Libert C

42-2400 was used in immunohistochemistry - paraffin section to determine the role of matrix metalloproteinase 3 in sepsis and inflammatory bowel disease.

Mon Jul 01 00:00:00 EDT 2013

Cell-cell contact-mediated hepatitis C virus (HCV) transfer, productive infection, and replication and their requirement for HCV receptors.

Journal of virology

Liu Z,He JJ

42-2400 was used in immunocytochemistry and western blot to study the mechanisms underlying cell-cell contact-mediated HCV transfer and infection.

Thu Aug 01 00:00:00 EDT 2013

Bifidobacteria stabilize claudins at tight junctions and prevent intestinal barrier dysfunction in mouse necrotizing enterocolitis.

The American journal of pathology

Bergmann KR,Liu SX,Tian R,Kushnir A,Turner JR,Li HL,Chou PM,Weber CR,De Plaen IG

42-2400 was used in immunohistochemistry - frozen section, immunohistochemistry (paraffin), and western blot to study the effects of intestinal barrier proteins in necrotizing enterocolitis and test if Bifidobacterium infantis modulates tight junction proteins.

Wed May 01 00:00:00 EDT 2013

Bifidobacteria stabilize claudins at tight junctions and prevent intestinal barrier dysfunction in mouse necrotizing enterocolitis.

The American journal of pathology

Bergmann KR,Liu SX,Tian R,Kushnir A,Turner JR,Li HL,Chou PM,Weber CR,De Plaen IG

Wed May 01 00:00:00 EDT 2013

Bifidobacteria stabilize claudins at tight junctions and prevent intestinal barrier dysfunction in mouse necrotizing enterocolitis.

The American journal of pathology

Bergmann KR,Liu SX,Tian R,Kushnir A,Turner JR,Li HL,Chou PM,Weber CR,De Plaen IG

Wed May 01 00:00:00 EDT 2013

Matrigel improves functional properties of primary human salivary gland cells.

Tissue engineering. Part A

Maria OM,Zeitouni A,Gologan O,Tran SD

42-2400 was used in immunohistochemistry to characterize a graft source from human salivary glands.

Sun May 01 00:00:00 EDT 2011

Matrigel improves functional properties of primary human salivary gland cells.

Tissue engineering. Part A

Maria OM,Zeitouni A,Gologan O,Tran SD

42-2400 was used in immunohistochemistry to characterize a graft source from human salivary glands.

Sun May 01 00:00:00 EDT 2011

Distribution of tight junction proteins in adult human salivary glands.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Maria OM,Kim JW,Gerstenhaber JA,Baum BJ,Tran SD

Published figure using Occludin polyclonal antibody (Product # 42-2400) in Immunohistochemistry

Mon Dec 01 00:00:00 EST 2008

Bioinformatics

Protein Aliases: Occludin; phosphatase 1, regulatory subunit 115; tight junction protein occludin; tight junction structural protein

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Gene Aliases: BLCPMG; OCLN; PPP1R115

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UniProt ID: (Dog) Q28269, (Human) Q16625

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Entrez Gene ID: (Dog) 403844, (Human) 100506658

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Function(s)

protein binding thiopurine S-methyltransferase activity protein domain specific binding general transcription factor

Process(es)

positive regulation of lamellipodium assembly positive regulation of microtubule polymerization bicellular tight junction assembly positive regulation of wound healing protein localization to cell leading edge protein complex assembly methylation cell-cell junction organization S-adenosylhomocysteine metabolic process S-adenosylmethionine metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Occludin Polyclonal Antibody

Occludin Antibody (42-2400) in ICC/IF

Product Details

42-2400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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